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Identification of the Differentially Expressed Genes of Hanwoo During the Growth Stage by Subtractive cDNA Hybridization

Subtraction 기법을 이용한 한우 성장 단계 특이 발현 유전자 탐색

  • Jang, Y.S. (National Livestock Research Institute, RDA) ;
  • Kim, T.H. (National Livestock Research Institute, RDA) ;
  • Yoon, D.H. (National Livestock Research Institute, RDA) ;
  • Park, E.W. (National Livestock Research Institute, RDA) ;
  • Cheong, I.C. (National Livestock Research Institute, RDA) ;
  • Jo, J.K. (Department of animal science, Kyungpook National University)
  • 장요순 (농촌진흥청 축산기술연구소) ;
  • 김태헌 (농촌진흥청 축산기술연구소) ;
  • 윤두학 (농촌진흥청 축산기술연구소) ;
  • 박응우 (농촌진흥청 축산기술연구소) ;
  • 정일정 (농촌진흥청 축산기술연구소) ;
  • 조진기 (경북대학교 동물공학과)
  • Published : 2002.02.28

Abstract

To identify the differentially expressed genes at growth stage of Hanwoo, we constructed the subtractive cDNA library from loin mRNA of 12- and 24-month old Hanwoo by PCR-based subtraction. The fourteen genes were confirmed by sequencing and reverse northern blot analysis, and they were selected as candidate of putative genes differentially expressed at the growth stage of Hanwoo. Three subtracted cDNA fragments that expressed specific signal to cDNA probe for 6-month-old loin of Hanwoo were highly homologous to those of the genes encoding EPV 20, Ca2+ATPase, and TCTP, respectively. The nine cDNA clones showed intense signal to cDNA probe from 12-month-old loin of Hanwoo, and highly homologus to those of genes encoding VCP, HSP 70, aldolase A, MSSK1, GM-2 activator protein, ryanodine receptor, acidic ribosomal phosphoprotein p1, ADP/ATP translocase, and UCP 2, respectively. Two subtracted cDNA clones that expressed specific signal to cDNA probes for 12- and 24-month-old loin of Hanwoo were detected. One of them was highly homologus to the gene encoding ferrochelatase and the other was highly homologus to the gene encoding ADRP.

Keywords

Hanwoo;Growth stage;Differentially expressed gene;Subtraction

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