Effects of Feeding Heat Treated Protein and Mineral Complex on In Vitro Fermentation Characteristics, Milk Production and Composition of Holstein Dairy Cows

열처리 단백질-광물질 복합제제 첨가가 In Vitro 발효성상과 착유우의 유량 및 유성분에 미치는 영향

  • Choi, N.J. (School of Agricultural Biotechnology, Seoul National University) ;
  • Bae, G.S. (Department of Animal Science and Technology, Chung-Ang University) ;
  • Nam, K.P. (Department of Animal Science and Technology, Chung-Ang University) ;
  • Chang, M.B. (Department of Animal Science and Technology, Chung-Ang University) ;
  • Um, J.S. (EUNJIN International Co., Ltd.) ;
  • Ko, J.Y. (Nonghyup Feed INC.) ;
  • Ha, J.K. (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2002.10.31


This study, consisting of two experiments, was conducted to determine the effects of feeding heat treated protein and mineral complex (HPM) on milk production and composition, and ruminal fermentation of Holstein dairy cows. In in vitro experiment, HPM levels were 0, 0.2, 1 and 2%, and Timothy hay, which was substrate, was milled as 1 mm size, and the effects of HPM on pH, ammonia and VFA were analyzed after incubation times of 0, 6, 12, 24 and 48 h, respectively. The pH and ammonia production were not significantly different between treatments during the incubation. In addition, generally, total VFA and individual VFA were not affected by HPM on 0, 6 and 24 h. While, total VFA and individual VFA were increased in 0.2% and 1% of HPM supplemented treatments, but decreased in 2% of HPM treatment compared with control on 12 h. On 48 h, total VFA and individual VFA were increased in HPM treatments compared to control (P<0.05). However, A/P ratio was not affected by HPM supplementation. Gas production was higher in HPM treatment compared to control on 24 h (P<0.05) and 48 h (P<0.05). In lactating experiment, fourteen lactating Holstein cows were used for 4 months in a cross over experimental design. There were two treatments; no added HPM as a control and 0.2% of HPM added as a test treatment. Daily milk yield (P<0.001), 4% FCM (P<0.001), milk protein (P<0.05) and SNF (solid not fat; P<0.05) were increased in HPM treatment compared to control. While, milk fat, MUN (milk urea nitrogen) and SCC (somatic cell count) were not significantly different between treatments.


Milk yield;Milk composition;Heat treated protein and mineral complex(HPM);Ruminal fermentation


  1. AOAC. 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC.
  2. Bach, A. and Stern, M. D. 1999. Effects of different levels of methionine and ruminally undegradable protein on the amino acid profile of effluent from continuous culture fermenters. J. Anim. Sci. 77:3377-3384.
  3. Beuvink, J. M., Spoelstra, S. F. and Hogendorp, R. J. 1992. An automated method ofr measuring the time course of gas production of feedstuffs incubated with buffered rumen fluid. Neth. J. Agri. Sci. 40:401-407.
  4. Britton, R. A. and Klopfenstein, T. J. 1986. Zinc treated soybean meal : A method to increase bypass. Beef Cattle Report. pp. 45-47.
  5. Chaney, A. L. and Marbach, E. P. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130-132.
  6. Duncan, D. B. 1955. Multiple range and multiple F test. Biometrics 11:1-42.
  7. Erasmus, L. J., Botha, P. M. and Meissner, H. H. 1994. Effect of protein source on ruminal fermentation and passage of amino acids to the small intestine of lactating cows. J. Dairy Sci. 77:3655-3665.
  8. Erdman, R. A. and Vandersall, J. H. 1983. Effect of rumen protein degradability on milk yield of dairy cows in early lactation. J. Dairy Sci. 66:1873-1880.
  9. Erwin, E. S., Marco, S. J. and Emery, E. M. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44:1768.
  10. Gibson, S. A. and Macfarlane, G. T. 1988. Characterization of proteases formed by bacteroids fragilis. J. Gen. Microbiol. 134:2231-2240.
  11. Grummer, R. R., Slark, K., Bertics, S. J., Luck, M. L. and Barmore, J. A. 1996. Soybeans versus animal sources of rumen-undegradable protein and fat for early lactation dairy cows. J. Dairy Sci. 79:1809-1816.
  12. Hof, G., Vervoorn, M. D., Lenaers, P. J. and Taminga, S. 1997. Milk urea nitrogen as a tool to monitor the protein nutrition of dairy cows. J. Dairy Sci. 80:3333-3340.
  13. McDougall, H. 1948. Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochemical J. 43:99-109.
  14. Plegge, S. D., Berger, L. L. and Gahey, Jr. G. C. 1985. Effect of roasting temperature on the proportion of soybean meal nitrogen escaping degradation in the rumen. J. Anim. Sci. 61: 1211-1218.
  15. Robinson, P. H. and Kennelly, J. J. 1988. Influence of intake of rumen undegradable protein on milk production of late lactation Holstein cows. J. Dairy Sci. 71:2135-2142.
  16. Rooke, J. A., Brook, I. M. and Armstrong, D. G. 1983. The digestion of untreated and formal- dehyde-treated soybean and rapeseed meals by cattle fed a basal silage diet. J. Agric. Sci. 100:329-342.
  17. SAS. 1989. User's Guide : Statistics, Version 6 Edition, SAS Inst., Inc., Cary, NC.
  18. Spears, J. W., Clark, J. H. and Hatfield, E. E. 1985. Nitrogen utilization and ruminal fermen- tation in steers fed soybean meal treated with formaldehyde. J. Anim. Sci. 60:1072-1080.
  19. Spears, J. W., Hartfield, E. E. and Forves, R. M. 1979. Nickel for ruminants. II. Influence of dietary nickel on performance and metabolic parameters. J. Anim. Sci. 48:649-652.
  20. Shepers, A. J. and Meijer, R. G. M. 1998. Evaluation of the utilization of dietary nitrogen by dairy cows based on urea concentration in milk. J. Dairy Sci. 81:579-584.
  21. Van Soest, P. J., Robertson, J. D. and Lewis, B. A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.
  22. Williams, A., Amat-Marco, M. and Collins, M. D. 1996. Pylogenetic analysis of Butyrivibrio strains reveals three distinct groups of species within the Clostridium subphylm of gram-positive bacteria. Int. J. Syst. Bacteriol. 46:195-199.
  23. Wohlt, J. E., Chmiel, S. L., Zajac, P. K., Backer, L., Blethen, D. B. and Evans, J. L. 1991. Dry matter intake, milk yield and composition, and nitrogen use in Holstein cows fed soybean, fish, or corn gluten meals. J. Dairy Sci. 74:1609-1622.
  24. Woodward, S. L., Auldist, M. J., Laboyrie, P. J. and Jansen, E. B. L. 1999. Effect of Lotus corniculatus and condensed tannins on milk yield and milk composition of dairy cows. Proc. New Zealand Soc. Anim. Prod. 59:152-155.
  25. 김 원, 김현진, 이성훈, 장문백, 맹원제. 2001. Canola seed의 가공방법에 따른 반추위 영양소 분해율 빛 발효특성 평가에 관한 연구. 동물자원과학회지. 43(6):841-858.
  26. 정유석, 하종규, 이성실, 김창현. 1993. 금속이온이 반추위내 미생물의 발효 성상 및 단백질 분해효소의 활력에 미치는 영향. 한국낙농학회지. 15(4):227-239.
  27. 최유지, 최낙진, 박성호, 송재용, 엄재상, 고종열, 하종규. 2002. 패스틴$^{\circledR}$ 첨가가 단백질 분해율과 반추위 발효 및 영양소 소화율에 미치는 영향. 동물자원과학회지. 44(5):557-568.
  28. 황일환. 1999. 반추위 혼합 미생물이 섬유소 분해 및 발효특성에 미치는 요인. 서울대학교 대학원. 석사학위논문.