Analysis of Melanocortin Receptor 1 (MC1R) Genotype in Korean Brindle Cattle and Korean Cattle with Dark Muzzle

칡소와 비경흑색 한우의 Melanocortin Receptor 1 (MC1R) 유전자형 분석

  • Lee, S.S. (National Jeju Agricultural Experiment Station) ;
  • Yang, B.S. (National Jeju Agricultural Experiment Station) ;
  • Yang, Y.H. (Department of Animal Biotechnology, Cheju National University) ;
  • Gang, S.Y. (National Jeju Agricultural Experiment Station) ;
  • Ko, S.B. (National Jeju Agricultural Experiment Station) ;
  • Jeong, J.K. (National Jeju Agricultural Experiment Station) ;
  • Oh, W.Y. (National Livestock Research Institute) ;
  • Oh, S.J. (National Livestock Research Institute) ;
  • Kim, K.I. (Department of Animal Biotechnology, Cheju National University)
  • 이성수 (농촌진흥청 제주농업시험장) ;
  • 양보석 (농촌진흥청 제주농업시험장) ;
  • 양영훈 (제주대학교 동물자원과학과) ;
  • 강승률 (농촌진흥청 제주농업시험장) ;
  • 고성봉 (농촌진흥청 제주농업시험장) ;
  • 정진관 (농촌진흥청 제주농업시험장) ;
  • 오운용 (농촌진흥청 축산기술연구소) ;
  • 오성종 (농촌진흥청 축산기술연구소) ;
  • 김규일 (제주대학교 동물자원과학과)
  • Published : 2002.02.28


PCR-RFLP analysis was carried out to investigate the genotype of Melanocortin receptor 1 (MC1R) gene in Korean Brindle Cattle and Korean Cattle with dark muzzle, which are coat color and muzzle pigmentation variants of Korean Cattle, respectively. Allelic variants of MC1R in cattle were analyzed by digestion with BsrFⅠ, AciⅠ. Among six genotypes, $E^D/E^D,\;E^D/E^+,\;E^D/e,\;E^+/E^+,\;E^+$/e and e/e, detected in cattle, only two genotypes, $E^+/E^+\;and\;E^+$/e, were observed in Korean Brindle Cattle, probably reflecting the necessary of $E^+$ allele for the expression of black brindle coat color. As in Korean Cattle with light muzzle, the $E^+$/e and e/e genotypes were detected in Korean Cattle with dark muzzle. The $E^+$ and e alleles frequencies in two populations of Korean Cattle with dark muzzle and with light muzzle were 0.37, 0.63 and 0,11, 0.89, respectively. Although the frequency of $E^+$ allele in Korean Cattle with dark muzzle was higher than in Korean Cattle with light muzzle, the $E^+$ allele was not completely associated with dark muzzle pigmentation. The results of this experiment indicate that the difference of MC1R genotype and frequency may be useful for fixation of coat color in Korean Cattle as well as Korean Brindle Cattle.


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