Optimal Culture Conditions for Production of Environment-Friendly Biosurfactant by Pseudomonas sp. EL-G527

Pseudomonas sp. EL-G527에 의한 환경친화성 생물계면활성제의 생산최적조건

  • 차미선 (부산대학교 미생물학과) ;
  • 임은경 (부산대학교 미생물학과) ;
  • 이근희 (부산대학교 미생물학과) ;
  • 조순자 (부산대학교 미생물학과) ;
  • 손홍주 (밀양대학교 생물공학과) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 2002.03.01


A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH$_4$NO$_3$, 0.3% KH$_2$PO$_4$, 0.3% $K_2$HPO$_4$, 0.02% MgSO$_4$ㆍ7$H_2O$, 0.0025% CaCk$_2$ㆍ6$H_2O$, 0.0015% FeSO$_4$ㆍ7$H_2O$ in 1$\ell$ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 $m\ell$ of the same medium in 250 $m\ell$ flask. They were cultivated at 3$0^{\circ}C$ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.



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