Immuno Activation of Lectin-Conjugated Praecoxin A on IL-6, IL-12 Expression

  • Joo, Seong-Soo (Division of Immunology, College of Pharmacy, Chung-Ang University) ;
  • Chang, Jae-Kwon (Division of Immunology, College of Pharmacy, Chung-Ang University) ;
  • Park, Jeong-Hwan (Division of Immunology, College of Pharmacy, Chung-Ang University) ;
  • Kang, Hee-Chul (Division of Immunology, College of Pharmacy, Chung-Ang University) ;
  • Lee, Do-Ik (Division of Immunology, College of Pharmacy, Chung-Ang University)
  • Published : 2002.12.01

Abstract

Lectin-conjugated praecoxin A is a compound, which is combined Wheat Germ Agglutinin (WGA) Lectin with praecoxin A and also known to have an anti-tumor activity. In our lab, in order to investigate its immune reaction other than the anti-tumor activity ever known, we examined cytokines such as IL-6 and IL-12 through their mRNA expressions, which are generally secreted by macrophage both in vivo and in vitro. To analyze, we used RT-PCR for total RNAs of macrophages. As a result, we obtained that both in vitro and in vivo, lectin-conjugated praecoxin A showed an interesting increase on IL-6 and IL-12 even though it may be little hard to say the conjugated form is absolutely more effective than that of lectin or praecoxin A alone for immune response activities. Those results suggest that the conjugated form may give an additional opportunity in a future therapeutic use over its immuno activation properties.

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