Isolation and Properties of Cytotoxic Polyene Antibiotics Produced by Myxococcus stipitatus JW117.

Myxococcus stipitatus JW117이 생산하는 Polyene계 세포독성 물질의 분리 및 특성

  • 안종웅 (한국화학연구원 생명의약부) ;
  • 최상운 (한국화학연구원 생명의약부) ;
  • 권호정 (세종대학교 공과대학 생명공학과)
  • Published : 2002.06.01

Abstract

Drug resistance is one of the most significant impediments to successful chemotherapy of cancer. Multidrug-resistance (MDR) is characterized by decreased cellular sensitivity to anticancer agents due to the overexpression of P-glycoprotein. By employing adriamycin-resistance CL02 cancer cells, we undertook the screening for agents which were effective to multidrug-resistant cancer cells. As a result, a myxobacterial strain JW117 was selected for study since the solvent extract of cell mass of the strain was found to exhibit significant activity against the CL02 cancer cells. Cytotoxicity-guided chromatographic fractionation led to the isolation of phenalamides $A_2$ and $A_3$. The producing organism was identified as Myxococcus stipitatus by taxonomic comparison with type strains of Myxococcus sp. as well as its morphological and physiological characteristics. Phenalamides$ A_1$,$ A_2$ and $A_3$ were as active against drug-resistant cancer cells CL02 and CP70 as against the corresponding sensitive cells with $IC_{50}$ values ranging from 0.23~0.57 $\mu\textrm{g}$/ml.

Keywords

Myxococcus stipitatus;phenalamides;mdr-cancer cell;cytotoxicity

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