Isolation and Identification of Thermostable \beta-glycosidase-producing Microorganism from Hot Spring of Volcanic Area at Atagawa in Japan.

일본의 Atagawa 온천지대에서 분리한 내열성 \beta-glycosidase 생성균주의 분리 및 동정

  • 남은숙 (한국방송통신대학교) ;
  • 최종우 (한국방송통신대학교) ;
  • 차성관 (한국식품개발연구원) ;
  • 안종건 (한국방송통신대학교)
  • Published : 2002.06.01

Abstract

This study was performed to obtain the thermostable $\beta$-glycosidase producing bacteria from hot spring of volcanic area at Atagawa in Japan. KNOUC 202 was selected because it showed thermostable $\beta$-glycosidase activity in sodium phosphate buffer(pH 6.8) at $70^{\circ}C$ for 4h, and it was identified. The strain was aerobic, asporogenic bacilli, immobile, gram negative, catalase positive, oxidase positive, and pigment-producing. Optimum growth was at $70~72^{\circ}C$, pH 7.0~7.2, and it could grow in the presence of 3% NaCl. The main fatty acids in cell were iso-15:0 and iso-l7:0. 16S rRNA sequence of KNOUC 202 showed 99.9% similarity with that of Thermus thermophilus ATCC 27634(HB8). Based on morphological, physiological, biochemical characteristics, cellular fatty acids profile and 16S rRNA sequence analysis, KNOUC 202 was identified as Thermus thermophilus.

Keywords

Thermostable \beta-glycosidase;Thermus thermophilus;hot spring

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