Isolation and Identification of Lactic Acid Bacteria Inhibiting Gastro-intestinal Pathogenic Bacteria of Domestic Animal.

가축 소화기 병원성 세균을 저해하는 유산균의 분리 및 동정

  • Lee, Jae-Yeon (Biotopia Co.) ;
  • Hwang, Kyo-Yeol (Biotopia Co.) ;
  • Kim, Hyun-Soo (Biotopia Co.) ;
  • Kim, Geun (Department of Genetic Engineering,The University of Suwon) ;
  • Sung, Soo-Il (Department of Biology,The University of Suwon)
  • 이재연 ((주)바이오토피아) ;
  • 황교열 ((주)바이오토피아) ;
  • 김현수 ((주)바이오토피아) ;
  • 김근 (수원대학교 유전공학과) ;
  • 성수일 (수원대학교 생물학과)
  • Published : 2002.06.01

Abstract

To isolate probiotic lactic acid bacteria having superior inhibitory activities against animal gastro-intestinal pathogenic bacteria such as Salmonella gallinarum, Staphylococcus aureus and Escherichia coli, 130 strains were initially isolated from the small intestines of Korean native chickens and 7 lactic acid bacteria were finally selected. By using API CHL kit and 16S rRNA sequencing method, the selected lactic acid bacteria were found to be belonged to genus Lactobacillus except BD14 identified as Pediococcus pentosaceus. Especially, Lactobacillus pentosus K34 showed the highest resistancy to both of HCl and bile salt, as well as the highest inhibitory activities against S. gallinarum, S. aureus and E. coli. All the selected strains were sensitive to various antibiotics such as neomycin, erythromycin, cephalosporin, amoxicillin/clavulanic acid, ampicillin, oxytetracycline, but resistant to ciprofloxacin. All the selected strains except BL strain were resistant to colistin and streptomycin, and BD14, BD16, K34 strains were resistant to gentamicin.

Keywords

Gastrointestinal pathogenic bacteria;inhibition;isolation and identification;lactic acid bacteria

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