Isolation and Enzyme Production of a Xylanase-producing Strain, Bacillus sp. AMX-4.

Xylanase를 생산하는 Bacillus sp. AMX-4 균주의 분리와 효소 생산성

  • 윤기홍 (우송대학교 식품생명과학부) ;
  • 설숙자 (우송대학교 식품생명과학부) ;
  • 조효찬 (우송대학교 식품생명과학부) ;
  • 이미성 (씨티씨바이오 중앙연구소) ;
  • 최준호 (씨티씨바이오 중앙연구소) ;
  • 조기행 (씨티씨바이오 중앙연구소)
  • Published : 2002.06.01

Abstract

A bacterium producing the extracellular xylanase was isolated from soil and has been identified as a Bacillus sp. strain. The isolate, named Bacillus sp. AMX-4, was shown to be similar to B. subtilis strain on the basis of its chemical compositions. The xylanase of culture supernatant was most active at 50℃ and pH 6.0. The additional carbon sources including monosaccharides, disaccharides, wheat bran, and rice straw increased the enzyme productivity. Especially, the maximum xylanase productivity was reached 29.2 units/ml in LB medium supplemented with 1.5% (w/v) xylose, which was 16-folds more than that in LB medium. As the results of investigating the effects of xylose on cell growth and xylanase productivity of Bacillus sp. AMX-4, increase of xylanase production was owing to the induction of xylanase biosynthesis. It was also found that the enzyme production was in association with the growth of Bacillus sp. AMX-4.

Keywords

Bacillus;identification;xylose;xylanase production

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