Identification of the Cell-envelope Proteinase of Lactic Acid Bacteria Isolated from Kimchi.

김치 유래 젖산균의 Cell-envelope Proteinase 존재 확인

  • 이유진 (경기대학교 식품생물공학과) ;
  • 최재연 (경기대학교 식품생물공학과) ;
  • 이형주 (서울대학교 식품공학과) ;
  • 장해춘 (조선대학교 식품영양학과) ;
  • 김정환 (경상대학교 식품공학과) ;
  • 정대균 (경희대학교 유전공학과 및 유전공학연구소) ;
  • 김영석 (이화여자대학교 식품영양학과) ;
  • 김소미 (제주대학교 아열대 원예산업 연구센터) ;
  • 이종훈 (경기대학교 식품생물공학과)
  • Published : 2002.06.01

Abstract

The partial 165 rDNA sequences of 6 lactic acid bacterial strains isolated from Kimchi were determined. Two strains were Leuconostoc mesenteroides and the rest were incorrectly classified and turned out to be Lactobacillus. As the case of dairy lactic acid bacteria, the strains isolated from Kimchi also had cell-envelope proteinase (CEP) activity. As the result of partial CEP gene amplification with CEP-specific primers, the expected 1.2-kb amplificate was obtained not from Leu. mesenteroides but from Lactobacillus strains. The deduced amino acid sequence of PCR product amplified from the genomic DNA of Lactobacillus pentosus KFR1821 showed 95% and 92% homology with those of PrtPs from Lactococcus lactis subsp. cremoris and Lactobacillus paracasei subsp. paracasei, respectively. The PCR amplificate was used as a probe and the result of Southern hybridization illuminated the location of CEP gene in chromosomal DNA of Lb. pentosus KFR1821.

Keywords

Cell-envelope proteinase;Kimchi;Leuconostoc;Lactobacillus

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