Isolation and characteristics of yellow-pigment producing mutants of Monascus anka.

Monascus anka로부터 황색소 생성 변이주의 분리 및 특성

  • 이호재 (동의공업대학 식품생명과학과) ;
  • 이형주 (서울대학교 식품공학과)
  • Published : 2002.06.01

Abstract

To produce yellow pigment selectively, mutants were induced from Monascus anka Nakazawa et Sato IFO 4478 (KCCM 11832 strain), and their characteristics were evaluated. Five kinds of auxotrophic mutants which required amino acids for growth and pigmentation, were isolated through a series of mutagenic treatments. Especially, asparagine auxotroph Y7 produced high ratio of yellow pigment. This mutant showed all the morphological characteristics of Monascuceae but the shape of colony and the diameter of conidia. Mutant Y7 was propagated by sexual reproduction more often than asexual reproduction, which could be effective in production of pigments. Yellow pigment produced extracellularly by the mutant Y7 was more soluble in polar solvents such as ethanol and water than in nonpolar solvents. Its productivity of yellow pigment was 2.2 times higher in the mutant Y7 than in parents. In addition, its yellow pigment showed characteristics of maximum absorption at 373 nm. Moreover, the hue of pigment produced by the mutant Y7 was bright yellow, and it was stable through the subculture over 10 generations.

Keywords

Monascus anka Nakazawa et Sato IFO 4478;mutant;yellow pigment;auxotroph

References

  1. J. Chem. Soc. The Chemistry of fungi Fielding, B. C.;J. S. E. Holker;D. F. Jones;A. D. G. Powell;K. W. Richimond;A. Robertson;W. B. Whalley https://doi.org/10.1039/jr9610004579
  2. J. Gen. Appl. Microbiol. v.2 Taxonomical studies on genus Rhizopus Inui, T.;Y. Takeda;H. Iizuka https://doi.org/10.2323/jgam.2.1
  3. Plant Physiol v.60 Pigmentation and antibacterial activity of fast neutron and X-ray induced strains of Monascus purpureus Went Wong, H. C.;Y. S. Bau https://doi.org/10.1104/pp.60.4.578
  4. J. Chin. Agr. Chem. Soc. v.8 Mycological study of Monascus anka Su, Y. C.;W. L. Chen;H. Y. Fang
  5. Nippon Nongeikagaku Kaishi v.60 Production of red pigment by Monascus sp. and its mutant Tsukioka, M.;T. Hiroi;T. Suzuki;T. Konno https://doi.org/10.1271/nogeikagaku1924.60.451
  6. J. Chin. Agr. Chem. Soc. v.14 Studies on the production of anka-pigment Su, Y. C.;M. Katayama
  7. Nippon Nongeikagaku Kaishi v.42 Asperyellone, a new yellow pigment produced by Aspergillus awamori and Aspergillus niger Arima, K.;J. H. Yu.;g. Tamura
  8. Appl. Environ. Microbiol v.43 Production of extracellular pigment by a mutant of Monascus kaoliang sp. nov. Lin, C. F.;H. Iizuka
  9. J. Chem. Soc. The biosynthesis of fungal metabolites Hadfield, J. R.;J. S. E. Holker;D. N. Stanway
  10. Agr. Biol. Chem. v.43 Hyperpigment productive mutant Monascus anka for solid culture Hiroi, T.;T. Shima;T. Suzuki;M. Tsukioka;O. Nagahiro https://doi.org/10.1271/bbb1961.43.1975
  11. Kor. J. Appl. Microbiol. and Biotechnol. v.8 Studies on the yellow pigment produced by Monascus sp. CS-2 Kim, H. S.;U. Chang;H. I. Yi;J. C. Bae;J. H. Yu
  12. Appl. Environ. Microbiol. v.47 Pigment production from immobilized Monascus sp. utilizing polymeric resin adsorption Evans, P. J.;H. Y. Wang
  13. Tetrahedron Lett. no.5 Fidlding, B. C.;E. J. Haws;J. S. E. Holker;A. D. G. Powell;A. Robertson;D. N. Stanway;W. B. Whalley
  14. J. Food. Sci. v.48 Production of red water soluble Monascus pigments Wong, H. C.;P. E. Koehler https://doi.org/10.1111/j.1365-2621.1983.tb09191.x
  15. J. Bacteriol v.122 Sexyal reproductive cycle of Monascus in submerged shaken culture Carel, M.;D. Shepherd
  16. J. Ferment. Technol. v.51 Isolation and cultural conditions of Monascus sp. for the production of pigment in a submerged culture Lin, C. F.
  17. US Pat. Water soluble Monascus pigment Yamaguchi, Y.
  18. Kor. J. Appl. Microbiol. Biotechnol. v.18 Isolation and identification of Streptomyces californicus KS-89 produced bluish purple pigment Ryu, B. H.;Y. E. Chi;B. G. Park;W. Y. Park;D. G. Kim
  19. Kor. J. Appl. Microbiol. Bioeng. v.11 Fermentative production of anka pigments (Monascus-Pigments) Su, Y. C.
  20. Agr. Biol. Chem. v.31 Asperyellone, a new yellow pigment of Aspergillus awamori and Aspergillus niger Yu, J. H.;G. Tamura;N. Takahashi;K. Arima https://doi.org/10.1271/bbb1961.31.831
  21. J. Chem. Soc. Studies in relation to biosynthesis Birch, A. J.;A. Cassera;P. Fitton;J. S. E. Holker;H. Smith;G. A. Thompson;W. B. Whalley https://doi.org/10.1039/jr9620003583
  22. Phytochemistry v.12 Isolation and structure of ankaflavin: a new pigment from Monascus anka Manchand, P. S.;W. B. Whalley;F. C. Chen https://doi.org/10.1016/0031-9422(73)80470-4
  23. Kor. J. Food. Sci. Technol. v.9 Studies on production and characteristics of edible red color pigment produced by mold (Monascus sp.) Kim, C. S.;S. H. Ree;I. Kim