Effects of Cultural Conditions on the In Vitro Propagation of Plantlets derived from Apical Meristem in Ipomoea batatas L.

고구마 정단분열조직 유래 식물체의 기내 증식에 미치는 배양조건의 영향

  • Eun, Jong-Seon (Research Institute of Bioindustry, Chonbuk Univ.) ;
  • Park, Jong-Suk (Research Institute of Bioindustry, Chonbuk Univ.) ;
  • Kim, Young-Seon (Dept. of Ornametal Horticulture Industry, Namdo Provincial College of Jeonnam)
  • 은종선 (전북대학교 농과대학 생물산업연구소) ;
  • 박종숙 (전북대학교 농과대학 생물산업연구소) ;
  • 김영선 (남도대학 관상원예산업과)
  • Published : 2002.03.01


Sweet potato is a crop vegetatively propagated by vine cuttings, an ineffective method for maintaining pathogene-free stock plants. As an alternative method, single-node cultures of virus-free plantlets derived from apical meristem in sweet potato (cv. Yulmi) was examined. Effective pH range, sugar concentration and nodal order were investigated to establish an in vitro mass propagation system with high quality virus-free stock plantlets to farmhouse. Although the plantlets grew at wide range of pH, the most effective pH of the medium was 4.8 in single-node cultures. High sugar concentration of 60∼80 g/L resulted in increased growth response in shoot length, root length, number of node, leaf area and fresh and dry weight of shoot and root, whereas reducing sugar contents below 6% was showed reduced growth response. The first node including meristem tip was the best for the rapid growth of plantlets and the other nodes also showed a very similar growth response. Uniform plantlet can be obtained massively at the same time by culture of single node except for the first node including meristem tip. In conclusion, the most effective pH range and sugar concentration of medium for the growth of plantlets via single-node cultures was 4.8, 60∼80 g/L respectively. The first node was the best for the rapid propagation of plantlets in nodal order.


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