Saccharomyces cerevisiae KNU5377 with Multiple Stress Tolerance and its Potential as a Worldwide On-site Industrial Strain for Alcohol Fermentation

  • Paik, Sang-Kyoo (Department of Microbiology, School of Life Science and Biotechnology, Kyungpook National University) ;
  • Ingnyol Jin (Department of Microbiology, School of Life Science and Biotechnology, Kyungpook National University) ;
  • Yun, Hae-Sun (Department of Microbiology, School of Life Science and Biotechnology, Kyungpook National University) ;
  • Park, Sae-Hun (Department of Microbiology, School of Life Science and Biotechnology, Kyungpook National University) ;
  • Shin, Seong-Chul (Department of Microbiology, School of Life Science and Biotechnology, Kyungpook National University) ;
  • Kim, Jae-Wan (R&D Center, Jinro Ltd.) ;
  • Shin, Ki-Sun (KCTC, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jung-Sook (KCTC, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Yong-Ha
  • Published : 2002.12.01

Abstract

Saccharomyces cerevisiae KNU5377 was examined to assay the recovering capacity against heat and other stressors. Along with a particular fermentation ability that is able to produce ethanol even at high temperature such as $40^{\circ}C$ with a comparable rate to the fermentation at $33^{\circ}C$, this strain also exhibited higher viability than a reference strain owing to its own thermotolerance that conferred the survival after the severe heat shock at $60^{\circ}C$ for 30 minutes. Furthermore, this strain showed outstanding tolerances against $H_2O_2$, ethanol and some chemical compounds. But, especially due to the thermotolerance, this strain has been suspected of other species of yeast. However, ITS (internally transcribed spacer) 1 and 2 sequencing data confirmed this strain was a typical strain of S. cerevisiae. The outstanding tolerances to various environmental stressors Indicate this S. cerevisiae KNU5377 is enough to use both as an on-site potential strain for world-wide alcohol fermentation industry and as a model strain for researches into the routes to acquire the tolerance to various stressors.

Keywords

Stress tolerance;Saccharomyces cerevisiae;ITS sequence

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