Isolation and Identification of Anticariotic Compound from Sophora flavescens Ait.

고삼으로부터 항우식활성 물질의 분리

  • 이현옥 (원광보건대학 치위생과) ;
  • 한동민 (원광대학교 자연과학기술학부) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2002.12.01


The purpose of this study is to investigate anticariotic activity of the ethyl acetate soluble extract of Sophora flavescens Ait. for the prevention of dental caries and glucosyltransferase activity caused by Streptococcus mutans. The fraction 5-4-3 showed strong growth inhibition activity against Streptococcus mutans (MIC, 3.13 $\mu\textrm{g}$/ml). The glucosyltransferase activity of the active fraction 5-4-3 inhibited the formation of glucan and showed 77% of the antiproliferative effect at 100 $\mu\textrm{g}$/ml (P<0.05). Two flavanones, (2S)-2'-methoxy kurarinone (1) and (+)-kurarinone (2), were isolated from the active fraction 5-4-3 of the ethyl acetate soluble extract of S. flavescens Ait. Their structures were elucidated using spectroscopic methods.


Streptococcus mutans;glucosyltransferase;Sophora flavescens Ait.;(2S)-2'-methoxykurarinone;(+)-kurarinone


  1. Infect. Immun v.25 Fraction properties of glucans produced by Streptococcus mutans Inoue,M.;T.Koga.
  2. Yakhak Hoeji. v.45 Isolation of antimicrobial compound from the ethyl acetate extract of Sophora flavescens. Lee,H.O.;N.K.Park;S.I.Jeong;Y.C.Kim;S.H.Back.
  3. Caries. Res. v.30 Inhibitory effects of funoran on the adherence and colonization of mutans streptococci Saeki,Y.;T.Kato;Y.Naito;I.Takazoe;K.Okuda
  4. Infect. Immunol v.61 Antigenicity and immunogenicity of a synthetic peptide derived from a glucanbinding domain of mutans streptococcal glucosyltransferase. Smith,D.J.;M.Taubman.
  5. Infect. Immun. v.37 Purification and properties of glucosyltransferase responsible for water-insoluble glucan synthesis from Streptococcus mutans Fukui,K.;T.Moriyama;Y.Miyake;K.Mizutani;O.Tanaka
  6. FEBS Lett. v.30 Evidence for the presence of two distinct sites of sucrose hydrolysis and glucosyItransferase activities on 1,3-alpha-D-Glucan synthase of Streptococcus mutans Yamashita,Y.;N.Hanada;M.Itoh-Andoh;T.Takehara
  7. Korean J. Food & Nutr v.12 Antibacterial and antifungal effect by Artemisia lavandulaefolia essential oil. Lee,H.O.;K.Y.Han;D.M.Han.
  8. J. Nat. Prod. v.61 A new prenylated flavonoid from the root of Sophora flavescens. Woo,E.R.;J.H.Kwak.;H.J.Kim;H.K.Park.
  9. J. Dent. Res. v.63 Virulence factors of Streptococcus mutans and dental caries prevention Hamada,S.;T.Koga;T.Ooshima.
  10. J. Dent. Res v.55 Variations in surface polymers of Streptococcus mutans. J. Dent. Res Ellwood,D.C.;J.K.Baird;J.R.Hunter;V.M.C.Longyear
  11. J. Korea Acad. of Dent. Health v.20 Antibacterial effect of some flavonoids against cariogenic bacteria Chung,D.I.;J.S.Ro;K.W.Chung
  12. Clinical Traditional Herbalogy Shin Min Kyo
  13. Korean J. Food & Nutr. v.23 Effects of solvents and extracting condition on the antimicrobial activity of Salviae miltiorrhizae Radix (Salvia miltiorrhiza) extract Mok.J.S.;U.Y.Park;Y.M.Kim;D.S.Chang
  14. Korea J. Food Sci. Technol v.30 Isolation of on anticariogenic compound from Magnoliae Bark. Lee,Y.S.;H.J.Park.J.S.You;H.H.Park;I.B.Kwon;H.Y.Lee.
  15. Arch. Oral. Biol. v.53 Decrease in cariogenicity of a mutans of Streptococcus mutans Stoppelaar,D.E.;J.D.Konig;K.G.Plasschaert
  16. Bull. Tokyo Dent. Coll. v.35 Effect of seaweed extracts on Streptococcus sobrinus adsorption to saliva-coated hydroxyapatite. Saeki,Y.
  17. J. Nat. Prod. v.63 Cytotoxic lavanduly from Sophora flavanones Kang.T.H.;S.J.Jeong;W.K.Ko;N.Y.Kim;B.H.Lee;T.M.Inagaki;R.Higuchi;Y.C.Kim
  18. Oral Microbiol. Immunol. v.11 Purification and properties of extracellular glucosyltransferase from Streptococcous bovis Uezono,Y.;H.Tsumori;A.Shimamura; H.Mukasa
  19. Infect. Immunol v.63 Streptococcus mutans mutant that synthesizes elevated levels of intracellular polysaccharide is hypercariogenic in vivo. Spatafora,G;K.Rocher;D.A.Barnard
  20. Planta Med. v.44 Dental caries prevention by traditional Chinese medicines. Part II. Potent antibacterial action of Magnoliae cortex extracts against Streptococcus mutans Namba,T.M.;Tsunezuka;H.Hattori
  21. Ph. D. thesis, Kangweon National Univ Studies on Glucosyltransferase Inhibitors from Cacao bean husk. Kwon,I.B.
  22. J. Kor. Aca. Dental Health. v.15 The effect of NaCI and bamboo salt on the growth of various oral bacteria. Shon,W.S;Y.C.Yoo;C.Y.Kim.