Degradation Characteristics of Methyl Ethyl Ketone and Methyl Isobuthyl Ketone by Pseudomonas putida KT-3.

Pseudomonas putida KT-3의 Methyl Ethyl Ketone 및 Methyl Isobuthyl Ketone 분해 특성

  • 김민주 (바이오세인트㈜ 환경생명공학연구소) ;
  • 이태호 (바이오세인트㈜ 환경생명공학연구소) ;
  • 이경미 (바이오세인트㈜ 환경생명공학연구소) ;
  • 류희욱 (바이오세인트㈜ 환경생명공학연구소, 숭실대학교 환경 화학공학과) ;
  • 조경숙 (이화여자대학교 환경학과)
  • Published : 2002.12.01

Abstract

Methyl ethyl ketone (MEK) and methyl isobutyl ketone (MIBK) have been widely used as solvents in various industries. Biodegradation of MEK and MIBK by Pseudomonas putida KT-3, which could utilize MEK or MIBK as a sole carbon source, was characterized, and the cosubstrate interaction in MEK/MIBK mixture was also studied. Within the range of initial MEK concentration (from 0.5 to 5.5 mM), an increased substrate concentration increased the specific degradation rate of MEK by P putida KT-3 (from 3.15 to 10.58 mmol/g DCW$\cdot$h), but the rate sightly increased at 11.0 mM of initial MEK concentation (11.28 mmol/g DCW$\cdot$h). The similar degradation rates of MIBK (4.69-4.92 mmol/g DCW$\cdot$h) were obtained at more than 3.0 mM of initial MIBK concentation. Kinetic analysis on the degradation of MEK/MIBK mixture by P. putida KT-3 showed that MEK or MIBK acted as a competitive inhibitor. Maximum degradation rate ($V_{max}$), saturation constant ($K_{m}$) and inhibition constant ($K_{1}$) were as follows: $V_{max,MEK}$=12.94 mmol/g DCW$\cdot$h; $K_{m,MEK}$=1.72 mmol/L; $K_{l,MEK}$=1.30 mmol/L; $V_{max,MIBK}$=5.00 mmol/g-DCW$\cdot$h; $K_{m,MIBK}$=0.42 mmol/L; $K_{l,MEK}$=0.77 mmol/L.

Keywords

Methyl ethyl ketone;methyl isobutyl ketone;Pseudomonas putida;biodegradation;mixture gas;inhibition

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