Effects of Xylooligosaccharides on the Growth of Intestinal Microflora.

자일로올리고당이 장내 세균에 미치는 영향

  • 류보경 (대한제당㈜ 중앙연구소) ;
  • 이지완 (대한제당㈜ 중앙연구소) ;
  • 이창승 (대한제당㈜ 중앙연구소) ;
  • 현승일 (대한제당㈜ 중앙연구소) ;
  • 박윤제 (대한제당㈜ 중앙연구소) ;
  • 안준배 (대한제당㈜ 중앙연구소) ;
  • 양창근 (대한제당㈜ 중앙연구소) ;
  • 윤세왕 (대한제당㈜ 중앙연구소)
  • Published : 2002.12.01

Abstract

To investigate the effects of xylooligosaccharides on the in vitro growth of intestinal bacteria, various species were cultivated individually on the m-PYF medium containing a carbon source (0.5% w/v) such as xylooligosaccharides, isomaltooligosaccharides, fructooligosaccharides and sucrose, respectively. The health-promoting microorganisms such as Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum, Lactobacillus casei and Lactobacillus acidophilus grew more effectively by xylooligosaccharides than by other carbon source, though xylooligosaccharides inhibited the growth of Clostridium perfringens, Bacteroides fragilis, Escherichia coli, Staphylococcus aureus and Salmonella typhumurium. At the mixed culture xylooligosaccharides exerted a preferential stimulatory effects on numbers of the health-promoting microorganisms, while xylooligosaccharides inhibited populations of potential pathogens at relatively low level. Xylooligosaccharides also maintained the acidity of culture with Streptococcus mutans, caries-inducing bacteria, over pH 5.0. These results suggest that xylooligosaccharides selectively promote the growth of the health-promoting microorganisms in human intestine and prevent caries by inhibiting acid production from Streptococcu mutans.

Keywords

Xylooligosaccharides;intestinal microflora;health-promoting microorganisms;Streptococcus mutans

References

  1. J. Microbiol. Biotechnol. v.7 Bifidogenic effect of glucooligosaccharide prepared from glucose by extrusion process Ahn,J.B.;J.K.Hwang;C.T.Kim;K.H.Lee;J.H.Park
  2. J. Indust. Microbiol. v.6 Bifidobacteria and their role in human health Mitsuoka,T. https://doi.org/10.1007/BF01575871
  3. Proceedings of the International Symposium on Probiotic Researchens on Lactic Acid Bacteria Production of xylooligosaccharide from the comcob xylan using the Clostridium thermocellum xylanase from recombinant Bacillus subtilis Oh,H.G.;J.W.Lee;C.S.Lee;Y.J.Park;U.T.Lee;C.K.Yang;S.W.Yoon
  4. J. Pham. Phamacol. v.52 Extract of Juglandaceae regia inhibition growth, in-vitro adherence, acid production and aggregation of Streptococcus mutans Jagtap,A.G.;S.G.Karkera https://doi.org/10.1211/0022357001773751
  5. Korean J. food Sci. Technol. v.32 Effects of ultrafiltration on the production of xylooloigosaccharides Park,Y.J.;H.G.Oh;J.W.Lee;C.S.Lee;U.T.Lee;B.K.Rhew;C.K.Yang;S.W.Yoon
  6. Korean. J. Food Sci. Technol. v.33 Physicochemical properties of xylooligosaccharide as food material Park,Y.J.;J.W.Lee;C.S.Lee;B.K.Rhew;C.K.Yang
  7. 暘內菌の世界 光岡知足
  8. 酒類産業 v.19 Properties and using of functional sweet food Lee,S.P.
  9. New Food Industry v.42 機能性 食品(1):世界的な廣かがりの狀況 藤田哲
  10. Human Microbial Ecology Control of the large bowel microflora Drasar,B.S.;A.K.Roberts
  11. J. Food Hyg. Safety v.14 Bacterial reverse mutation assay of xylooligosaccharede Oh,H.G.;Y.J.Park;U.T.Lee;J.W.Lee;C.S.Lee;B.K.Rhew;C.K.Yang;S.W.Yoon;B.H.Kang
  12. Dietary Fiber in Health and Disease Health benefits of non-digestible oli-gosaccharides Roberfoid,M.B.
  13. Appl. Environ. Microbiology v.59 Utilization of xylooligosaccharides by selected ruminal bacteria Cotta,M.A.
  14. J. Food Hyg. Safety v.14 Actue oral toxicity of xylooligosaccharid in rats Park,Y.J.;H.G.Oh;U.T.Lee;J.W.Lee;C.S.Lee;B.K.Rhew;C.K.Yang;S.W.Yoon;B.H.Kang
  15. Proceedings of the Society for Experimental Biology and Medicine v.90 Microbiological studies on growth factor for L. bifidus var. pennsylvanicus Gyorgy,P.;R.F.Norris;C.S.Rose
  16. Bifidobact. Microflora. v.9 Effect if xylooligosaccharide on the growth of bifidobacteria Okazaki,M.;S.Fujikawa;N.Matsumoto https://doi.org/10.12938/bifidus1982.9.2_77
  17. Korean J. Food Sci. Technol. v.25 Screening of th foodstuffs influencing the growth of Bifidobacterium spp. and Clostridium perfringens. Park,J.H.;N.S.Han;J.Y.Yoo;H.K.Shin;Y.J.Koo
  18. J. Food Hyg. Safety v.15 Subacute toxicity of xylooligosaccharide in rats Park,Y.J.;U.T.Lee;J.W.Lee;C.S.Lee;B.K.Rhew;C.K.Yang;S.W.Yoon;B.H.Kang
  19. British Journal of Nutrition v.81 Functional food properties of non-digestible oli-gosaccharides: a consensus report from the ENDO project (DGXII AIRII-CT94-1095) Loo,J.van;J.Cummings;N.Delzenne;H.Englyst;A.Franck;M.Hopkins;N.Kok;G.Macfarlane;D.Newton;M.Quigley;M.Roberfroid;T.van Vliet;E.van den Heuvel https://doi.org/10.1017/S0007114599000252
  20. J. Dairy Sci. v.79 Effect of bifidogenic factors on growth characteristics of bifidobacteria in infant formulas Dubey,U.K.;V.V.Mistry https://doi.org/10.3168/jds.S0022-0302(96)76469-X
  21. Journal of Dairy Research v.65 Concentrated bovin colostral whey proteins from Streptococcus mutans/Strep. Sobrinus immunized cows inhibit the adherence of Strep. mutans and promote the aggregation of mutans streptococci Loimaranta,V.;A.Carlen;J.Olsson;J.Tennovuo;E.L.Syvaoja;H.Korhonen https://doi.org/10.1017/S0022029998003069
  22. Annales de Microbiologie v.129B Amino acid produced by bifidobacteria and some clostridia Matteuzzi,D.;F.Crociani,;O.Emardi
  23. Kor. J. Appl. Microbiol. Biotechnol. v.20 Growth effect of branched oligosaccharides on principle intestinal bacteria Park,J.H.;J.Y.Yoo;O.H.Shin;H.K.Shin;S.J.Lee;K.H.Park
  24. Human intestinal microflora in health and disease Normal indigenous intestinal flora Finegold,S.M.;V.L.Sutter;G.E.Mathisen
  25. Japanese Technology Reviews v.3 Xylooligosaccharides Kunimasa,K.;F.Shigeaki
  26. Gastroenterology v.108 Selectives stimulation of bifidobacteria in the human colon by oilgofructose and inulin Gibson,G.R.;E.R.Beatty;X.Wang;J.H.Cummings https://doi.org/10.1016/0016-5085(95)90192-2
  27. Xylans and xylanases Visser,J.;G.Beldman;M.A.Kusterws;A.G.J.Voragen
  28. Appl. Microbiol. Biotechnol. v.49 Oat β-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains Jaskari,J.;P.Kontula;A.Siitonen;H. Jousimies-Somer;T. Mattila-Sandholm;K.Poutanen https://doi.org/10.1007/s002530051155
  29. Korean. J. Food Sci. Technol. v.27 Effect of dietary xylooligosaccharide on indigestion and retarding effect of bile acid movement across a dialysis membrane Joo,G.J.;I.K.Rhee;S.O.Kim;S.J.Rhee
  30. Bifidobact. Microfl. v.9 Taxonomy and ecology of bifidobacteria Mitsuoka,T.
  31. Proceedings of KSBB Spring Scientific Meeting Xylan extraction from corncob Lee,J.W.;Y.J.Park;H.G.Oh;C.S.Lee;U.T.Lee;C.K.Yang;S.W.Yoon