Optimal Conditions for the Production of Intracellular Cytosine Deaminase from Chromobacterium violaceum YK 391.

Chromobacterium violaceum YK 391의 세포내 Cytosine Deaminase의 생성 최적조건

  • Kim, Jung (Department of Dental Hygiene) ;
  • Kim, Hyun-Soo (Department of Microbiology, Keimyung University) ;
  • Yoo, Dae-Sik (Department of Microbiology, Keimyung University)
  • 김정 (수원여자대학 치위생과) ;
  • 김현수 (계명대학교 자연과학대학 미생물학과) ;
  • 유대식 (계명대학교 자연과학대학 미생물학과)
  • Published : 2002.12.01

Abstract

Cytosine deaminase (cytosine aminohydrolase, EC 3.5.4.1) stoichiometrically catalyzes the hydrolytic deamination of cytosine and 5-fluorocytosine to uracil and 5-fluorouracil, respectively. Optimal medium compositions for production of cytosine deaminase from Chromobacterium violaceum YK 391 were 0.75% soluble starch, 1.5% peptone, 0.1% meat extract, 0.1% yeast extract, 0.01% NaCl, 0.01% $MgCl_2{\cdot}7H_2O$ and 0.05% $K_2HPO_4$. The optimal pH of medium and incubation temperature were 7.0 and $30^{\circ}C$, respectively. C. violaceum reached stationary phase after 30 hr, and produced a maximum cytosine deaminase (120 units/ml) after 72 h in batch culture.

Keywords

Cytosine deaminase;Chromobacterium violaceum YK 391

References

  1. Z. Biol. v.79 Uber das verhalten von pyrimidinderivaten in den organismen. I. Einfluss von hefe auf pyrimidinderivate Hahn,A.;W.Lintzel
  2. Kor. J. Microbiol. v.30 Some properites of partially purified cytosine deaminase from Bacillus stearothermophilus Yu,T.S.;T.H.Kim
  3. Kor. J. Appl. Microbiol. Biotechnol. v.25 Isolation and identification of bacterium producing extracellular cytosine deaminase Yu,T.S.;T.H.Kim
  4. Biochem. v.10 Baker's yeast cytosine deaminase. Some enzymic properties and allosteric inhibition by nucleosides and nucleotides Ipata,P.L.;F.Marmochii;G.Magni;R.Felio;G.Polidoro https://doi.org/10.1021/bi00799a018
  5. Korean J. Biotechnol. Bioeng. v.12 Optimization of extracellular cytosine deaminase production from Chromobacterium. violaceum YK 391. Yu,T.S.;T.H.Kim
  6. Agr. Biol. Chem. v.39 Purification of cytosine deaminase from Serratia marcescens Sakai,T.;T.S.Yu;H.Tabe;S.Omata https://doi.org/10.1271/bbb1961.39.1623
  7. Agr. Biol. Chem. v.39 Purification of cytosine deaminase from Pseudomonas aureofaciens Sakai,T.;T.S.Yu;K.Taniguchi;S.Omata https://doi.org/10.1271/bbb1961.39.2015
  8. Z. Biol. v.83 Uber das verhalten von pyrimidin derivaten in den organismen. II. Einwirkung von Bacterium coli auf uracil und cytosin Hahn,a.;L.Schafer
  9. Biochem. Pharmac. v.15 The metabolism of 5-fluorocytosine-$2^{14}$C and of cytosine-$^{14}C$C in the rat and the disposition on 5-fluorocytosine-$2^{14}$C in man. Koechin,B.A.;F.Rubio;S.Palmer;T.Gabriel;R.Duschinsky https://doi.org/10.1016/0006-2952(66)90254-1
  10. Experimentia v.33 Cytosine deaminase: Structure modification studies Yergatian,S.;J.B.Lee;;M.J.Geisow;J.C.Ireson https://doi.org/10.1007/BF01934002
  11. Kwahaknonzip Keimyung University v.3 Nutritional and cultural characteristics of cytosine deaminase from Pseudomonas aureofaciens Yu,T.S.
  12. J. Amer. Chem. Soc. v.73 On the cytosine deaminase of yeast Kream,J.;E.Chargaff
  13. J. Microbiol. Biotechnol. v.9 Purification and properties of extracellular cytosine deaminase from Chromobacterium. YK 391. Yu,T.S.;T.H.Kim
  14. Biochim. Biophys. Acta. v.719 Purification and some properties of cytosine deaminase from Salmonella typhimurium West,T.M.;S.Shanly;G.A.O'Donodvan https://doi.org/10.1016/0304-4165(82)90096-4
  15. J. Microbiol. v.36 Chemical modification of cytosine deaminase from Aspergillus fumigatus Tu,T.S.;T.H.Kim
  16. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of protein utilizing for the principle of protein-dye binding Bradford,B.M. https://doi.org/10.1016/0003-2697(76)90527-3
  17. J. Ferment. Bioeng. v.72 Purification and some some properties of cytosine deaminase from Aspergillus fumigatus Yu,T.S.;J.K.Kim;T.Katsuragi;T.Sakai;K.Tonomura https://doi.org/10.1016/0922-338X(91)90161-9