Characterization of Extracellular \alpha-galactosidase Produced by Streptomyces sp. YB-4.

균체외 \alpha-galactosidase를 생산하는 Streptomyces sp. YB-4의 분리 및 효소 특성

  • 김소영 (우송대학교 식품생명과학부) ;
  • 조기행 (씨티씨바이오 중앙연구소) ;
  • 김창진 (한국생명공학연구원) ;
  • 박동진 (한국생명공학연구원) ;
  • 윤기홍 (우송대학교 식품생명과학부)
  • Published : 2002.12.01

Abstract

A strain YB-4 producing the extracellular $\alpha$-galactosidase was isolated from soil, and has been identified as Streptomyces sp. on the basis of its cultural, morphological and physiological properties. The partially purified $\alpha$-galactosidase was most active on paranitrophenyl-$\alpha$-D-galactopyranoside at pH 6.0 and 6$0^{\circ}C$. The enzyme retained 90% of its maximum activity between pH 4.0 and pH 10.0 after pre-incubation for 1 h. The enzyme was able to hydrolyze oligomeric substrates such as melibiose, raffinose and stachyose to liberate galactose residue, indicating that the $\alpha$-galactosidase of Steptomyces sp. YB-4 hydrolyzed $\alpha$-1,6 linkage.

Keywords

Streptomyces;identification;\alpha-galactosidase;property;reaction product

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