Characterization of Extracellular \alpha-galactosidase Produced by Streptomyces sp. YB-4.

균체외 \alpha-galactosidase를 생산하는 Streptomyces sp. YB-4의 분리 및 효소 특성

  • 김소영 (우송대학교 식품생명과학부) ;
  • 조기행 (씨티씨바이오 중앙연구소) ;
  • 김창진 (한국생명공학연구원) ;
  • 박동진 (한국생명공학연구원) ;
  • 윤기홍 (우송대학교 식품생명과학부)
  • Published : 2002.12.01


A strain YB-4 producing the extracellular $\alpha$-galactosidase was isolated from soil, and has been identified as Streptomyces sp. on the basis of its cultural, morphological and physiological properties. The partially purified $\alpha$-galactosidase was most active on paranitrophenyl-$\alpha$-D-galactopyranoside at pH 6.0 and 6$0^{\circ}C$. The enzyme retained 90% of its maximum activity between pH 4.0 and pH 10.0 after pre-incubation for 1 h. The enzyme was able to hydrolyze oligomeric substrates such as melibiose, raffinose and stachyose to liberate galactose residue, indicating that the $\alpha$-galactosidase of Steptomyces sp. YB-4 hydrolyzed $\alpha$-1,6 linkage.


Streptomyces;identification;\alpha-galactosidase;property;reaction product


  1. Appl. Environ. Microbiol. v.59 Comparative study on the production of guarα-galactosidase by Saccharomyces cerevisiae SU50B and Hansenula polymorpha 8/2 in continuous cultures Giuseppin,M.L.;J.W.Almkerk;J.C.Heistek;C.T.Verrips
  2. FEMS Microbiol. Lett. v.192 α-Galactosidase a from Pseudomonas fluorescens subsp. cellulosa: cloning, high level expression and its role in galactomannan hydrolysis Halstead,J.R.;M.P.Fransen;R.Y.Eberhart;A.J.Park;H.J.Gilbert;G.P.Hazlewood
  3. J. Bacteriol. v.184 α-Galactosidase Aga27A, an enzymatic component of the Clostridium josui cellulosome Jindou,S.;K.Shuichi;F.Emi;F.Tsuchiyoshi;H.Hidenori;K.Tetsuya;S.Kazuo;O.Kunio
  4. Enzyme Microb. Technol. v.22 Characterization of galactosidases from Aspergillus niger: purification of a novel α-galactosidase activity Manzanares,P.;L. H. de Graff;J. Visser
  5. Prep. Biochem. v.20 Extracellualr α-galactosidase (EC from Aspergillus ficuum NRRL 3135: purification and characterization Zapter,I.G.;A.H.J.Ullah;R.J.Wodzinski
  6. Appl. Microbiol. Biotechnol. v.53 A comparison of enzyme-aided bleaching of softwood paper pulpusing combinations of xylanase, mannanase and α-galactosidase Clarke,J.H.;K.Davidson;J.E.Rixon;J.R.Halstead;M.P.Fransen;H.J.Gilbert;G.P.Hazlewood
  7. FEMS Microbiol. Lett. v.112 Purification and molecular properties of an α-galactosidase synthesized and secreted by Aspergillus nidulans Rios,S.;A.M.Pedregosa;I.F.Monostrol;F.Laborda
  8. Appl. Environ. Microbiol. v.65 Differential expression of three α-galactosidase genes and a single β-galactosidase gene from Aspergillus niger de Vries,R. P.;H. C.van den Broeck;E.Dekkers;P.Manzanares;L. H. de Graff;J. Visser
  9. Appl. Microbiol. Biotechnol. v.56 Purification and characterization of thermostable β-mannanase and α-galactosidase from Bacillus stearothermophilus Talbot,G.;J.Sygusch
  10. J. Gen. Microbiol. v.129 Numerical classification of Streptomyces and related genera Williams,S.T.;M.Goodfellow;G.Alderson;E.M.H.Wellington;P.H.A.Sneath;M.Sackin
  11. Appl. Microbiol. Biotechnol. v.65 Cloning of the gene encoding a novel thermostable α-galactosidase form Thermus brockianus ITI360 Fridjonsson,O.;H.Watzlawick;A.Gehweiler;T.Rohrhirsch;R.Mattes
  12. Acta Microbiol. Pol. v.50 Optimization of α-galactosidase production in Streptomyces erythrus Elshafei,A.M.;M.S.Foda;E.Abdel-Mobde;N.H.Ali
  13. Enzyme Microb. Technol. v.4 Immobilixed α-galactosidase in the sugar beet industry Linden,J.C.
  14. J. Biotechnol. v.8 Production of thermostable recombinant α-galactosidase suitable for raffinose elimination from sugar beet syrup Ganter,C.;A.Bock;P.Buckel;R.Mattes
  15. Biochem. J. v.219 Glycosidases induced in Aspergillus tamarii: Secreted α-D-galactosidase and beta-D-mannanase Civas,A.;R.Eberhard;P. Le Dizet;F.Patek
  16. Int. J. Syst. Bacteriol. v.16 Methods for the characterization of Streptomyces species Shirling,E.B.;D.Gottlieb
  17. Adv. Relat. Areas Mol. Biol. v.56 α-Galactosidase Dey,P.M.;E.D.Campillo
  18. Appl. Environ. Microbiol. v.67 Purification and characterization of the recombinant Thermus sp. strain T2 α-galactosidase expressed in Escherichia coli Ishiguro,M.;S.Kanedo;A.Kuno;Y.Koyama;S.Yoshida;G.G.Park;Y.Sakakibara;I.Kusakabe;H.Kobayashi
  19. Biosci. Biotechnol. Biochem. v.61 Purification, characterization and cDNA colning of novel α-galactosidase from Mortierella vinacea Shibuya,H.;H.Kobayashi;T.Sato;W.S.Kim;W.Yoshida;S.Kaneko;K.Kasamo;I.Kusakabe
  20. Appl. Environ. Microbiol. v.59 Conditions of formation, purification, and characterization of an α-galactosidase of Trichoderma reesei RUT C-30. Zeilinger,S.;D.Kristufek;I. Arisan-atack;R.Hodits;C.P.Kubicek
  21. Biotechnol. Appl. Biochem. v.17 Induction of α-galactosidase in Penicillium ochrochloron by guar(Cyamopsis tetragonoloba) gum Dey,P.M.;S.Patel;M.D.Brownleader
  22. Poult. Sci. v.74 Removal of the α-galactosides of sucrose from soybean meal using either ethanol extraction or exogenous alpha-galactosidase and broiler performance Irish,G.G.;G.W.Barbour;H.L.Classen;R.T.Tyler;M.R.Bedford
  23. Curr. Microbiol. v.38 α-Galactosidase of Bifidobacterium adolescentis DSM 20083 Leader,S.;W.Hartmeier;S.P.Mark
  24. Bergey's Manual of Systematic Bacteriology v.4 Suprageneric classification of Actinomyces Goodfellow,M.;T.Cross;H.A.Lechevalier
  25. Biotechnol. Appl. Biochem. v.28 α-Galactosidases of Penicillium simplicissimum: production, purification and characterization of the gene encoding AGLI Luonteri,E.;E.Alatalo;M.Siika-Aho;M.Penttila;M.Tenkanen
  26. Biochemistry(Moscow) v.56 Isolation and properties of α-galactosidase from Aspergillus awomori Neustroev,K.N.;A.S.Krylov;O.N.Abiroskina;L.M.Firsov;V.V.Nasonov;A.Y.Khorlin