Optimal Production and Characterization of Fibrinolytic Enzyme from Fomitella fraxinea Mycelia.

Fomitella fraxinea 균사체로부터 Fibrin분해효소의 최적생산 및 효소적 특성

  • 이종석 (부산대학교 자연과학대학 미생물학과) ;
  • 백형석 (부산대학교 자연과학대학 미생물학과) ;
  • 박상신 (동국대학교 자연과학대학 생명공학과)
  • Published : 2002.12.01


investigated to maximize the production of fibrinolytic enzyme from Fomitella fraxinea mycelia. Among the tested media, Coriolus versicolor medium (CVM) showed the highest production for the enzyme. 2% galactose, 0.6% yeast extract and 0.1% $NaNO_3$, 0.1% $K_2HPO_4$, and 0.05% $MgSO_4$.$7H_2O$ as carbon, nitrogen, phosphorus, and inorganic salt sources resulted in the maximum level of the enzyme activity, respectively. The enzyme production from F. fraxinea was reached to highest level after the cultivation for 10 days at $25^{\circ}C$ and pH 9. The enzyme activity of culture supernatant was most active at $40^{\circ}C$ and pH 10. The activity of the enzyme was inhibited by phenylmethylsulfonylfluoride and aprotinin, suggesting that it is a serine protease.


Fomitella fraxinea;fibrinolytic enzyme;optimal production


  1. Biochem. Biophy. Res. Commun. v.197 Purification and characterization of strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan Fujita,M.;K.Nomura;K.Hong;Y.Ito;A.Asada;S.Nishimuro https://doi.org/10.1006/bbrc.1993.2624
  2. Fibrinolysis v.1 Fibrinolysis, general aspects, characteristic features and perspectives, Int'l Committee for fibrinolysiskabi Prize Lecture Mullertz,S. https://doi.org/10.1016/0268-9499(87)90025-7
  3. Med. Clin. North Am. v.65 Haemostasis, thrombosis and thromboembolic disorder Harlan,J.M.;L.A.Harker
  4. Arch. Biochem. Biophys. v.40 The fibrin plate method for estimating fibrinolytic activity Astrup,T.;S.Mullertz https://doi.org/10.1016/0003-9861(52)90121-5
  5. Appl. Environ. Microbiol. v.62 Purification and Characterization of a Fibrinolytic Enzyme produced from Bacillus sp. Strain CK11-4 Screened from Chungkookjang Kim,W.K.;K.H.Choi;Y.T.Kim;H.H.Park;J.Y.Choi;Y.S.Lee;H.I.Oh;I.B.Kwon;S.Y.Lee
  6. Kor. J. Appl. Microbiol. Biotechnol. v.26 Medium optimization for fibernolytic enzyme by Bacillus subtilis KCK-7 isolated from Korean traditional Chungkookjang Lee,S.K.;S.Heo;D.H.Bae;K.H.Choi
  7. J. Gen. Microbiol. v.139 Identification of two laccase genes in the cultivated mushroom Agaricus bisporus Perry,C.R.;M.Smith;C.H.Britnell;D.A.Wood;C.F.Thurston https://doi.org/10.1099/00221287-139-6-1209
  8. Kor. J. Microbiol. v.22 Isolation and Characterization of Psudomonas sp. producing alkaline protease Lee,E.G.;E.H.Park;H.H.Lee;H.H.Hyun
  9. Kor. J. Mycol. v.29 Antioxidative activity and structural analysis of the steroid compound from Fomitella fraxinea Park,S.S.;J.S.Lee;K.G.Bae;K.H.Yu;H.C.Han;T.J.Min
  10. J. Nat. Prod. v.61 Fomitellic acid, triterpenoid inhibitors of eukaryotic DNA polymerases from a Basidiomycete, Fomitella fraxinea Tanaka,N.;A.Kitamura;Y.Mizushina;F.Sugawara;K.Sakaguchi
  11. Biosci. Biotech. Biochem. v.57 Characterization of potent fibrinolytic enzymes in earthworm. Lumbricus rebellus Nakajima,N.;H.Mihara;H.Sumi
  12. J. Biotech. v.24 Production and properties of an extracellular metalloprotease from a psychophilic Pseudomonas fluorescens Schinner,F.;R.Maregensin https://doi.org/10.1016/0168-1656(92)90125-S
  13. Kor. J. Appl. Microbiol. Biotechnol. v.26 Purification and characterization of fibrinolytic enzyme excreted by Bacillus subtilis K-54 isolated from Chung Guk Jang Yoo,C.K.;W.S.S.Heo;C.S.Lee;S.M.Kang
  14. Kor. J. Mycol. v.18 The full list of recorded mushrooms in Korea Lee,T.S.
  15. Biosci. Biotecnol. Biochem. v.63 A Fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea. Kim,J.H.;Y.S.Kim https://doi.org/10.1271/bbb.63.2130
  16. Kor. J. Mycol. v.23 Immunostimulating polysaccharides from the fruiting Bodies of Fomitella fraxinea(I)-characterization of polysaccharides extracted with neutral sodium chloride solution Cho,S.M.;J.H.Lee;S.B.Han;H.M.Kim;S.H.Yu;I.D.Yoo
  17. J. Biochem. v.118 Characterization of a thermostable lysinespecific metallopeptidase from the fruiting bodies of a basidiomycete, Grifola frondosa. Nonaka,T.;H.Ishikawa;Y.Tsumuraya;Y.Hashimoto;N.Dohmae https://doi.org/10.1093/jb/118.5.1014
  18. Chem. Pharm. Bull. v.29 Studies on fungal polysaccarides.ⅩⅩⅦ. Structural examination of a water-soluble, antitumor polysaccharides of Ganoderma lucidum Toshio,M.;N.Motohro https://doi.org/10.1248/cpb.29.3611
  19. J. Microbiol. Biotechnol. v.9 Anticomplementary properties of polysaccharides isolated from fruit bodies of mushroom Pleurotus ostreatus Kweon,M.H.;H.Jang;W.H.Lim;H.I.Chang;C.W.Kim;H.C.Yang;H.J.Hwang;H.C.Sung
  20. Biochemistry v.34 Identification of plasminogen binding region in streptokinase that is necessary for the creation of functional streptokinase-plas-minogen activator complex Reed,G.L.;L. F. Parhami-Seren;P.Kussie https://doi.org/10.1021/bi00032a021
  21. J. Microbiol. Biotechnol. v.11 Purification and characterization of a fibrinolytic enzyme from Bacillus sp. KDO-13 isolated from soybean paste Lee,S.K.;D.H.Bae;T.J.Kwon;S.B.Lee;H.H.Lee;J.H.Park;S.Heo;M.G.Jhonson
  22. Angiologia v.43 The relationship between lipid peroxidation and platelets aggregation in atherosclerotic patients Gomez,C.C.;R.Simoncarballo;A.C.Coma;T.Sanchez de Leon;D.E.Montero;P.R.Rodriguez
  23. Gene v.28 Production and secretion of porcine urokinase in Saccharomyces cerevisiae: characterization of the secreted gene product Zaworski,P.G.;K.R.Marotti;V.Mackay;C.Yip;G.S.Gill
  24. Appl. Microbiol. Biotechnol. v.28 Serine proteinase from Bacillus brevis Kalebina,T.S.;N.R.Galina;I.O.Seyakh;O.M.Khodova;I.S.Kulaev
  25. Mycologia v.90 Purification and partial characterization of a fibrinolytic protease in Pleurotus ostreatus Choi,H.S.;H.H.Shin https://doi.org/10.2307/3761226
  26. Am. J. Cardiol. v.59 Recombinant tissue plasminogen activator Sherry,S. https://doi.org/10.1016/0002-9149(87)91139-8
  27. Appl. Environ. Microbiol. v.64 Characterization of laccases and peroxidases from wood-rotting fungi (family Coprinaceae) Heinzkill,M.;L.Bech;T.Halkier;P.Schneider;T.Anke
  28. Eur. J. Biochem. v.239 Domain structure, stability and interactions in streptokinase Madved,L.V.;D.A.Solovjov;K.C.Ingham https://doi.org/10.1111/j.1432-1033.1996.0333u.x
  29. J. Microbiol. Biotechnol. v.7 Immunostimulating activity of polysaccharides from mycelia of Phellinus linteus grown under different culture conditions Lee,J.H.;S.M.Cho;H.M.Kim;N.D.Hong;I.D.Yoo
  30. J. Microbiol. Biotechnol. v.9 Purification and characterization of a iron-containing superoxide dismutase from lentinus edodes Park,S.S.;S.M.Hwang
  31. Master thesis, Korea University Anticoagulant activity of Extracts from the Mycelia of Phelinus linteus Kim,J.S.
  32. Basic Medical Biochemistry Marks,D.;A.Marks;C.Smith