Optimal Production and Characterization of Fibrinolytic Enzyme from Fomitella fraxinea Mycelia.

Fomitella fraxinea 균사체로부터 Fibrin분해효소의 최적생산 및 효소적 특성

  • 이종석 (부산대학교 자연과학대학 미생물학과) ;
  • 백형석 (부산대학교 자연과학대학 미생물학과) ;
  • 박상신 (동국대학교 자연과학대학 생명공학과)
  • Published : 2002.12.01

Abstract

investigated to maximize the production of fibrinolytic enzyme from Fomitella fraxinea mycelia. Among the tested media, Coriolus versicolor medium (CVM) showed the highest production for the enzyme. 2% galactose, 0.6% yeast extract and 0.1% $NaNO_3$, 0.1% $K_2HPO_4$, and 0.05% $MgSO_4$.$7H_2O$ as carbon, nitrogen, phosphorus, and inorganic salt sources resulted in the maximum level of the enzyme activity, respectively. The enzyme production from F. fraxinea was reached to highest level after the cultivation for 10 days at $25^{\circ}C$ and pH 9. The enzyme activity of culture supernatant was most active at $40^{\circ}C$ and pH 10. The activity of the enzyme was inhibited by phenylmethylsulfonylfluoride and aprotinin, suggesting that it is a serine protease.

Keywords

Fomitella fraxinea;fibrinolytic enzyme;optimal production

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