Isolation of a Specific Antigen Protein on Cell Membrane of Cochlodinium polykrikoides, Red Bloom

적조생물 Cochlodinium polykrikoides의 세포표면 특이항원 단백질의 분리

  • 김광현 (동의대학교 미생물학과) ;
  • 한창희 (동의대학교 생물학과) ;
  • 이재훈 (동의대학교 미생물학과) ;
  • 김병우 (동의대학교 미생물학과) ;
  • 이복규 (동의대학교 생물학과)
  • Published : 2002.12.01


To establish a rapidly immunochemical identification on a dinoflagellate, Cochlodinium polykrikoides, a specific antigenic protein as a maker on the cell membrane was isolated. The cell membranes of C. polykrikoides and Gymnodinium sangineum were harvested by centrifugation after osmotic shock. The membrane proteins of both cells were solubilized in 50 mM Na-carbonate contained 1 mM DTT, and separated the proteins on SDS-PACE. Immune-blot on the solubilized membrane proteins of the both cells was performed with antiserum against the solubilized membrane proteins of C. polykrikoides. A 120 kDa membrane protein of C. polykrikoides had remarkablely different antigenicity from that of G. sangineum.


Cochlodinium polykrikoides;identification;cell membrane;specific antigenic protein


  1. J. Fish. Sci. Technol. v.3 Indentification of Cochlodinium polykrikoides against Gymnodinium impudicum and Gymnodinium catenatum in field samples using FITC lectin probes Cho,E.S.;D.W.Kang;Y.C.Cho
  2. J. Eukaryotic Microbiol. v.41 Occurrence of the parasitic dinoflagellate Amoebophrya ceratii in Chesapeake populations of Gymnodinium sanguineum Coats,D.W.;K.R.Bockstahler
  3. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli,U.K.
  4. J. Exp. Marine Biol. Ecol. v.68 The original recurrent blooms of Gymnodinium sanguineum Hirasaka in a shallow coastal lagoon Voltolina,D.
  5. Natural phytoplankton community responses to a bloom of the toxic dinoflagellae Gymnodinium breve Davis off the North Carolina Coast v.61 West,T.L.;H.G.Marshall;P.A.Tester;Morgantown,W.Va(ed.)
  6. NATO ASI Ser. G Ecol. Sci. Bloom dynamic of the toxic dinoflagellate Gymnodinium catenatum, with Emphasis on Tasmanian and Spanish Coastal Waters Hallegraeff,G.M.;S.Fraga
  7. A global perspective in red tides Toxic algal bloom and red tides Anderson,D.M.;Okaichi,T.(ed.);D.M.Anderson(ed.);T.Nemato(ed.)
  8. Marine Biotech. v.3 Polymerase chain reaction in detection of Gymnodinium mikimotoi and Alexandrium minutum in field samples from Southwest India Godhe,A.S.;S.K.Otta;A.S.Rehnstam-Holm;I.Karunasager
  9. Marine Biol. v.116 Grazing to the mixotrophic dinoflagellate Gymnodinium sangunium on ciliate Chesapeake Bay Bockstahler,K.R.;D.W.Coats
  10. Bull. Nat. Fish. Res. Dev. Agency v.41 Red tide occurrence and succession of its causative organism in Jinhae Bay Park,J.S.;H.G.Kim;S.G.Lee
  11. Bull. Kor. Fish. Soc. v.29 Bioactive components from Red tides plakton, Cochlodinium polykrikoides Lee,J.S.
  12. J. Exp. Marine Biol. Ecol. v.186 The use of polyclonal antisera and blocking antibodies in the identification of marine dinoflagellates: species-specific antisera against Gymnodinium and Alexandrium Mendoza,H.;V.lopez-Rodas;S.Gonzalez-Gil;A.Aguilera
  13. Bull. Kor. Fish. Soc. v.14 On the Gymnodinium red tide in Jinhae Bay Cho,C.H.
  14. Oceanologia et Limnologia v.32 Evolution and causes of formation of Gymnodinium sanguineum Bloom in Yantai Sishili Bay Wu,Y.L.;C.X.Zhou;Y.S.Zhang;X.M.Pu;W.H,Li
  15. Bull. Kor. Fish. Soc. v.23 Coastal algal bloom caused by the cyst-forming dinoflagellates Kim,H.G.;J.S.Park;S.G.Lee
  16. Immunochemistry in practice(2nd ed.) Johnstone,A.;R.Thorpe
  17. Bull. Kor. Fish. Soc. v.29 The Occurrence of a Dinoflagellate Gymnodinium catenatum From Chinhae Bay, Korea Kim,H.G.;K.Matsuoka;S.G.Lee;K.H.An
  18. Methods in molecular biology v.3 Production of antibodies using proteins in gel Amero,S.A.;T.C.James;S.C.R.Elgin;Walker,J.M.(ed.)
  19. Bull. Kor. Fish. Soc. v.11 On the Gonyaulax red tide in Jinhae Bay Cho,C.H.
  20. Korea Phycologia v.40 Diurnal vertical migration of a harmful dinoflagellate, Cochlodinium polykrikoides (Dinophyceae) during a red tide in coastal waters of Namhae Island Park,J.G.;M.K.Jeong;J.A.Lee;K.J.Cho;O.S.Kwon
  21. Japan Soc. Water Environ. v.24 A study of the occurrence of Cochlodimium polykrikoides off the southern coast of Korea Kim.Y.S.;Y.S.Lee;J.S.Park;C.I,Baik
  22. Mar. Ecol. Prog. Ser. v.57 Immunochemical recognition of phytoplankton species Shapiro,L.P.;L.Campbell;E.M.Haugen
  23. Gram. Can. J. Microbiol. v.8 Studies of marine planktonic diatoms. I. Cyclotella nana (Husteldt) and Detommule conferracea (Cleve) Guillard,R.L.;J.H.Ryther
  24. Oceanologia et Limnologia v.32 Evolution and causes of formation of Gymnodinium sanguineum Bloom in Yantai Sishili Bay Wu,Y.L.;C.X.Zhou;Y.S.Zhang;X.M.Pu;W.H.Li
  25. Manual of clinical immunology(2nd ed.) Enzyme-linked immunosorbent assay Voller,A.;D.E.Bidwell;A.Baclett;Rose,N.R.(ed.);H.Friedman(ed.)
  26. Marine Ecology, Progress Series v.103 Detection of ichthyotoxic dinoflagellate Gyrodinium(cf.) aureolum and morphologically related Gymnodinium species using monoclonal antibodies: immunological tool Vrieling,E.G.;L.Peperzak;W.W.C.Gieskes;M.Veenhuis
  27. NATO ASI Ser. G Eco. Sci. v.1989 Bloom dynamics and physiology of Gymnodinium breve with emphasis Maxico Steidinger,K.A.;G.A.Vargo;P.A.Tester;C.R.Tomas
  28. Fish. Sci. v.63 Analysis of Gymnodinium catenatum dinophyceae using sequences of its regions and random amplified polymorphic DNA Adachi,M.;Y.Sako;Y.Ishida