Isolation of a Specific Antigen Protein on Cell Membrane of Cochlodinium polykrikoides, Red Bloom

적조생물 Cochlodinium polykrikoides의 세포표면 특이항원 단백질의 분리

  • 김광현 (동의대학교 미생물학과) ;
  • 한창희 (동의대학교 생물학과) ;
  • 이재훈 (동의대학교 미생물학과) ;
  • 김병우 (동의대학교 미생물학과) ;
  • 이복규 (동의대학교 생물학과)
  • Published : 2002.12.01

Abstract

To establish a rapidly immunochemical identification on a dinoflagellate, Cochlodinium polykrikoides, a specific antigenic protein as a maker on the cell membrane was isolated. The cell membranes of C. polykrikoides and Gymnodinium sangineum were harvested by centrifugation after osmotic shock. The membrane proteins of both cells were solubilized in 50 mM Na-carbonate contained 1 mM DTT, and separated the proteins on SDS-PACE. Immune-blot on the solubilized membrane proteins of the both cells was performed with antiserum against the solubilized membrane proteins of C. polykrikoides. A 120 kDa membrane protein of C. polykrikoides had remarkablely different antigenicity from that of G. sangineum.

Keywords

Cochlodinium polykrikoides;identification;cell membrane;specific antigenic protein

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