Characterization of Bacillus stearothermophilue Cyclodextrin Glucanotransferase that Expressed by Saccharomyces cerevisiae

Saccharomyces cerevisiae에서 발현된 Bacillus stearothermophilus Cyclodextrin Glucanotransferase의 특성

  • 박현이 (동의대학교 미생물학과) ;
  • 전숭종 (일본산업기술종합연구소) ;
  • 권현주 (미쯔비시화학생명과학연구소) ;
  • 남수완 (동의대학교 생명공학과) ;
  • 김한우 (동의대학교 미생물학과) ;
  • 김광현 (동의대학교 미생물학과) ;
  • 김병우 (동의대학교 미생물학과)
  • Published : 2002.12.01

Abstract

The cyclodextrin glucanotransferase (CGTase) gene from Bacillus stearothermophilus NO2 was expressed in Saccharomyces cerevisiae 2805 under the adhl promoter. The CGTase was purified from S. cerevisiae 2805/pVT-CGTS. The purified enzyme exhibited a optima of activity around pH 7.0 and $65^{\circ}C$. Thermal stability of the enzyme was increased fairly as compared with the CGTase of B. stearothermophilus NO2. The conversion yield of cyclodextrin (CD) and the production ratio of $\alpha$-, $\beta$,-, ${\gamma}$-CD from starch were showed similarly aspect to the CGTase of B. stearothermophilus NO2.

Keywords

Bacillus stearothermophilus;cyclodextrin glucanotransferase;Saccharomyces cerevisiae;thermal stability

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