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Characterization of Biodegradation of Highly Concentrated Phenol by Rhodococcus sp. EL-GT

Rhodococcus sp. EL-GT에 의한 고농도 페놀의 분해 특성

  • Park, Geun-Tae (Department of Microbiology, Pusan National University) ;
  • Cha, Mi-Sun (Department of Microbiology, Pusan National University) ;
  • Nam, Gui-Sook (Department of Microbiology, Pusan National University) ;
  • Cho, Sun-Ja (Department of Microbiology, Pusan National University) ;
  • Son, Hong-Joo (Department of Biotechnology, Miryang National University) ;
  • Lee, Geon (Department of Microbiology, Pusan National University) ;
  • Lee, Sang-Joon (Department of Microbiology, Pusan National University)
  • 박근태 (부산대학교 미생물학과) ;
  • 차미선 (부산대학교 미생물학과) ;
  • 남귀숙 (부산대학교 미생물학과) ;
  • 조순자 (부산대학교 미생물학과) ;
  • 손홍주 (밀양대학교 생물공학과) ;
  • 이건 (부산대학교 미생물학과) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 2002.09.01

Abstract

The isolated strain, Rhodococcus sp. EL-GT was able to degrade high phenol concentrations up to 10 mM within 24 hours in the medium consisting of 5.3 mM $KH_2PO_4$. 95 mM $Na_2HPO_4$, 18mM $NH_4NO_3$, 1 mM $MgSO_4{\cdot}7H_2O$,\;50{\mu}M CaCl_2$,\;0.5 {\mu}M FeCl_3$, initial pH 8.0, temperature $30^{\circ}C$ in rotary shaker at 200 rpm. This strain was good cell growth and phenol degradation in the alkaline pH range range, and the highest in the pH range of 7 to 9. The microorganism was able to grow at the various chlorinated phenols, benzene, toluene, and bunker-C oil. As Rhodococcus sp. EL-GT was good capable of attachment on the acryl media, it would be used as microorganism to consist of biofilm in wastewater treatment.

Keywords

Rhodococcus sp.;sludge;RBC;biofilm reactor

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