Damage of Perennial Ryegrass, Lolium perenne by Chestnut Brown Chafer, Adoretus tenuimaculatus (Coleoptera: Scarabaeidae) and Biological Control with Korean Isolate of Entomopathogenic Nematodes

주둥무늬차색풍뎅이(Adoretus tenuimacuiatus)에 의한 퍼레니얼라이그라스(Lolium perenne)피해와 한국산 곤충병원성 선충을 이용한 생물적 방제

  • 이동운 (경상대학교 농업생명과학연구원) ;
  • 추호렬 (경상대학교 농업생명과학연구원, 경상대학교 응용생명과학) ;
  • 신옥진 (진주컨트리클럽) ;
  • 윤재수 (상주대학교 잠사곤충자원학과) ;
  • 김영섭 (상주대학교 잠사곤충자원학과)
  • Published : 2002.09.01


The chestnut brown chafer, Adoretus tenuimaculatus Waterhouse, is serious insect pests in golf courses. Adults feed on the leaves of latifoliate trees but larvae feed on roots of turfgrases such as bentgrass, Agrostis spp. Damage of A. tenuimaculatus larvae was observed at the Jinju golf club which showed damage symptom on perennial ryegrass, Lolium perenne in tees and fairways in July, 2000. Damaged turf by A. tenuimaculatus larvae became yellowish and wilted. Symptom of laval damage of A. tenuimaculatus was similar to summer depression in warm season turfgrasses but not recovered by irrigation when Korean isolates of entomopathogenic nematodes were evaluated for the control of A. tenuimaculatus larvae in laboratory and field as a possible biological control agent. The nematodes used were Heterorhabditis bacteriophora Jeju strain, Hererorhabditis sp. Gyeongsan strain, Steinernema carpocapsae Pocheon strain, S.glaseri Dongrea strain, and S.longicaudum Nonsan strain. In the laboratory test H.bacreriophora Jeju strain and Heterorhabditis sp. Gyeongsan strain were highly effective for 3rd instars with 95% mortality. In the field test reduction rates of A.tenuimaculatus larvae were higher by ranging from 28 to 57% by H. bacteriophora Jeju strain, Heterorhabditis sp. Gyeongsan strain, and S.carpocapsae Pocheon strain compared to 7% by natural cause.


Heterorhabdiris;Steinernema;Adoretus tenuimaculatus;Ryegrass;Entomopathogenic nematode;Biological control;Heterorhabditis;Steinernema


  1. Fischer, P. and E. Ftihrer. 1990. Effect of soil acidity on the ento-mophilic nematode Steinernema kraussei Steiner. Biology and Fertility of Soils 9: 174-177 https://doi.org/10.1007/BF00335803
  2. Kaya, H.K. and R. Gaugler. 1993. Entomopathogenic nematodes. Annu. Rev. Entomol. 38: 181-206 https://doi.org/10.1146/annurev.en.38.010193.001145
  3. $Koppenh\ddot{o}fer$, A.M. and H.K. Kaya. 1997. Additive and Syner-gistic interaction between entomopathogenic nematodes and Bacillus thurineiensis for scarb grub control. Biol. Contr. 8: 131-137 https://doi.org/10.1006/bcon.1996.0498
  4. Lee, D.W., H.Y. Choo, J.M. Chung, S.M. Lee, J. Huh and Y.T. Sung. 1998. Vegetation of golf courses and local difference of feeding host plant to Adoretus tenuimacutatus Waterhouse (Coleoptera: Scarabaeidae). Kor. Turfgrass Sci. 12: 1-16
  5. Lee, D.W., H.Y. Choo, T.W. Lee, J.W. Park and T.W. Kweon. 1999. Spatial and temporal distribution of chestnut brown Chaf-er, Adoretus tenuifnaculatus (Coleoptera: Scarabaeidae) in golf courses. Kor. Turfgrass Sci. 13: 113-124
  6. Smits, P.H., G.L. Wiegers and H.J. Vlug. 1994. Selection of the parasitic nematodes for biological control of the garden chafer, Phyllopertha horticota. Entomol. exp. app. 70: 77-82 https://doi.org/10.1007/BF02380633
  7. Womersley, C.Z. 1990. Dehydration survival and anhydrobiotic potential. pp. 117-137. In Entomopathogenic nematodes in bi0-logical control, eds. by R. Gaugler and H.K. Kaya. 365 pp CRC Press, Boca Raton
  8. $Koppenh\ddot{o}fer,$ A.M., H.Y. Choo, H.K. Kaya, D.W. Lee and W.D. Gelemter. 1999. Increase field and greenhouse efficacy against scarab grubs with a combination of an entomopathogenic nema-tode and Bacillus thuringiensis. Biol. Contr. 14: 37-44 https://doi.org/10.1006/bcon.1998.0663
  9. Townsend, M.L., D.C. Steinkraus and D.T. Johnson. 1994. Mort-ality response of green June beetle (Coleoptera: Scarabaeidae) to four species of entomopathogenic nematodes. J. Entomol. Sci. 29: 268-275 https://doi.org/10.18474/0749-8004-29.2.268
  10. Choo, H.Y., D.W. Lee, S.M. Lee, T.W. Kweon, Y.T. Sung and P.Y. Cho. 1998. White gmbs in turfgrasses of golf courses and their seasonal density. Kor. Turfgrass Sci. 12: 225-236
  11. Forschler, B.T. and W.A. Gardner, 1991. Concentration-mortality response of Phyllophasa hirticula (Coleoptera: Scarabaeidae) to three entomogenous nematodes. J. Econ. Entomol. 84: 841-843 https://doi.org/10.1093/jee/84.3.841
  12. 1987. Turfgrass insects ofthe United States and Canada. 391 pp. Comell University Press, Ithaca
  13. Lee, S.M., D.W. Lee, H.Y. Choo, D.W. Kim and J.B. Kim. 1997b. Pathogenicity of entomopathogenic nematodes to some agro forest insect pests. Korean J. Soil Zoology. 2: 76-82
  14. Choo, H.Y. and H.K. Kaya. 1991. Influence ofsoil texture and Pr-esence of roots on host finding by Heterorhabditis bacterioph-ora. J. Invertebr. Pathol. 58: 279-280 https://doi.org/10.1016/0022-2011(91)90074-Z
  15. Choo, H.Y., H.H. Kim, D.W. Lee, S.M. Lee, S.H. Park, Y.M. Choo and J.G. Kirn. 2001. Practical utilization of entomopatho-genic nematodes, Steinernema carpocapsae Pocheon strain and Heterorhabditis bacteriophora Hamyang strain for control of chestnut insect pests. Korean J. Appl. Entomol. 40: 69-79
  16. Kim, H.K. 1991. Turfgrass science. 545 pp. Sunjinmunhwasa, Seoul
  17. Alm, S.R., T. Yeh, J.L. Hanula and R. Georgis. 1992. Biological control of Japanese, ohental, and black turfgrass atenius beetle (Coleoptera: Scarabaeidae) larvae with entomopathogenic ne-matodes (Nematoda: Steinernematidae, Heterorhabditidae). J. Econ. Entomol. 85: 1660-1665 https://doi.org/10.1093/jee/85.5.1660
  18. Lee, D.W., H.Y. Choo, H.K. Kaya, S.M. Lee, D.R. Smitly, H.K. Shin and C.G. Park. 2002. Laboratory and field evaluation of Korean entomopathogenic nematode isolates against the Orien-tal beetle, Exomala orientalis. J. Econ. Entomol. (In press)
  19. Lossbroek, T.G. and J. Theunissen. 1985. The entomogenous ne-matode Neoaplectana bibionis as a biological control agent of Asrotis seeetum in lettuce. Entomolo. exp. appl. 39: 261-264 https://doi.org/10.1111/j.1570-7458.1985.tb00468.x
  20. Hatsukade, M., Y. Kono and M. Yoshida. 1978. Studies on may beetles injurious to the turfgrass IX. Damage and seasonal Prev-alence of the chestnut brown chafer, Adoretus tenuimacutatus Waterhouse. Turfgrass Research 7: 55-61
  21. Lee, D.W., H.Y. Choo, J.M. Chung, S.M. Lee, T.W. Lee and Y.D. Park. 1997a. Host plants and preference ofbrown chafer, Ador-etus tenuimacutatus Waterhouse (Coleoptera: Scarabaeidae). Korean J. Appl. Entomol. 36: 156-165
  22. Stock, S.P., H.Y. Choo and H.K. Kaya. 1997. First record of Stei-nernema elaseri Steiner, 1929 (Nematoda: Steinemematidae) in Asia, with notes on intraspecific variation. Nematologica 43: 377-381 https://doi.org/10.1163/005025997X00111
  23. Choo, H.Y., H.K. Kaya, J. Huh, D.W. Lee, H.H. Kim, S.M. Lee and Y.M. Choo. 2002. Entomopathogenic nematodes (Steinern-ema spp. and Heterorhabditis bacteriophora) and a fungus Beauvena brongniartii for biological control ofthe white grubs, Ectinohoplia rufipes and Exomala orientatis, in Korean golf courses. BioControl 47: 177-192 https://doi.org/10.1023/A:1014559729607
  24. $Koppenh\ddot{o}fer,$ A.M. and H.K. Kaya. 1998. Synergism of imidac10-prid and an entomopathogenic nematode: a novel approach to white grub (Coleoptera: Scarabaeidae) control in turfgrass. J. Econ. Entomol. 91: 618-623 https://doi.org/10.1093/jee/91.3.618
  25. Cho, I.H. 1996. Practice and application of SAS. 665 pp. Sungan-dang Pub. Co. Seoul
  26. Gaugler, R. and G.M. Boush. 1978. Effects of ultraviolet radiation and sunlight on the entomogenous nematode, Neoaptectana carpocapsae. J. Invertebr. Pathol. 32: 291-296 https://doi.org/10.1016/0022-2011(78)90191-X
  27. $Koppenh\ddot{o}fer,$ A.M., R.S. Cowles, E.A. Cowles, E.M. Fuzy and L. Baumgartner. 2002. Comparison of neonicotinoid insecticide as synergists for entomopathogenic nematodes. Biol. Contr. (In press)
  28. Converse, V. and P.S. Grewal. 1998. Virulence of entomopath-ogenic nematodes to the westem masked chafer Cyclocephalahirta (Coleoptera: Scarabaeidae). J. Econ. Entomol. 91: 428-432 https://doi.org/10.1093/jee/91.2.428
  29. Molyneux, A.S. and R.A. Bedding. 1984. Influence of soil texture and moisture on the infectivity of Heterorhabditis sp. Dl and Steinernema elaseri for larvae of the sheep blowfly, Lucilia cuprina. Nematologica 30: 358-365 https://doi.org/10.1163/187529284X00266
  30. Choo, H.Y., D.W. Lee, J.W. Park and J.W. Lee. 1999. Compari-son of four major scarab beetles, Ectinohoplia rufipes, Adoretus tenuimaculatus, Exomala orientalis and Popillia quadriguttata in golf courses. Kor. Turfgrass Sci. 13: 101-112