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Protective Effect of Dietary Buchu (Chinese chives) Against Oxidative Damage from Aging and Ultraviolet Irradiation in ICR Mice Skin

  • Lee, Min-Ja ;
  • Ryu, Bog-Mi ;
  • Kim, Mi-Hyang ;
  • Lee, Yu-Soon ;
  • Moon, Gap-Soon
  • Published : 2002.09.01

Abstract

Protective effect of skin by antioxidative dietary buchu (Chinese chives, Allium tuberosum Router), was evaluated in ICR mice fed diets containing 2% or 5% buchu for 12 months. Lipid peroxidation and protein oxidation in skin, with or without ultraviolet B (UVB) irradiation, activities of antioxidative enzymes, total glutathione concentrations, and non-soluble collagen contents were measured. Dietary buchu decreased significantly in TBARS and protein carbonyl levels in skin compared to the control group, and were lower in those fed 5% than 2% buchu diet group. ICR mice exhibited an age-dependent decrease in antioxidative enzyme activities and total glutathione concentrations on the control diet, but in the groups fed buchu diet the enzyme activities and glu-tathione concentrations remained at youthful levels for most of the study. SOD, glutathione peroxidase, and catalase activities as well as total glutathione concentrations increased with time in the skins of the mice fed buchu diets. Lipid peroxidation and protein oxidation provoked by UVB irradiation on ICR mice skin homogenates were also significantly inhibited by dietary buchu. The buchu diets also decreased the formation of non-soluble collagen in mice skin, compared to the control group. These results suggest that antioxidative components and sulfur-compounds in buchu may confer protective effect against oxidative stress resulting from aging and exposure to ultraviolet irradiation.

Keywords

buchu;antiaging;lipid peroxidation;protein oxidation;antioxidative enzyme;UVB irradiation;collagen

References

  1. Chinese Medicine Dictionary. 1985. So-hawk-guan. Sanghae Scientific and Academic Publisher. p 838
  2. Pinto JT, Qiao C, Xing J, Rivlin RS, Protomastro ML, Weissler ML, Tao Y, Thaler H, Heston WDW. 1997. Effects of garlic thioallyl derivatives on growth, glutathione concentration, and polyamine formation of human prostate carcinoma cells in culture. The American Journal of Clinical Nutrition 66: 398-405
  3. Lee MJ, Ryu BM, Lee YS, Moon GS. 2002. Effect of long term buchu (Chinese chives) diet on antioxidative system of ICR mice. J Korean Soc Food Sci Nutr (in press)
  4. Mortensen A, Skibsted LH, Sampson J, Rice-Evans C, Everett SA. 1997. Comparative mechanisms and rates of free radical scavenging by carotenoid antioxidants. FEBS Letters 418: 91-97 https://doi.org/10.1016/S0014-5793(97)01355-0
  5. Yu BP. 1996. Aging and oxidative stress: Modulation by dietary restriction. Free Rad Biol Med 21: 651-668 https://doi.org/10.1016/0891-5849(96)00162-1
  6. Anatol K, Ulrike M, Sonke A, Amaar U, Charlotte L, Tomas MT, Ulrike B. 2001. Influence of vitamin E and C supplementation on lipoprotein oxidation in patients with Alzheimer's disease. Free Rad Biol Med 31: 345-354 https://doi.org/10.1016/S0891-5849(01)00595-0
  7. Rakesh PP, Brenda JB, Jack HC, Neil H, Marion K, Balaraman K, Dale AP, Stephen B, Victor DU. 2001. Antioxidant mechanisms of isoflavones in lipid systems: paradoxical effects of peroxyl radical scavenging. Free Rad Biol Med 31: 1570-1581 https://doi.org/10.1016/S0891-5849(01)00737-7
  8. Kameoka H, Miyake A. 1974. The constituents of the steam volatile oil from Allium tuberosum Rotter. Nippon Nogeikagaku Kaishi 48: 385 https://doi.org/10.1271/nogeikagaku1924.48.385
  9. Black SH. 1987. Potential involvement of free radical reactions in ultraviolet light-mediated cutaneous damage. Photochem Photobiol 46: 213-221 https://doi.org/10.1111/j.1751-1097.1987.tb04759.x
  10. Danno K, Horio T, Takigawa M, Imamura S. 1984. Role of oxygen intermediates in UV-induced epidermal cell injury. The Journal of Investigative Dermatology 83: 166-168 https://doi.org/10.1111/1523-1747.ep12263506
  11. Johnson EY, Lookingbill DP. 1984. Sunscreen use and sun exposure : Trends in a white population. Archives of Dermatology 120: 727-731 https://doi.org/10.1001/archderm.120.6.727
  12. Elwood JM, Gallager RP, Hill GB. 1985. Sunburn, suntan and the risk of cutaneous malignant melanoma. The Western Canada Melanoma Study. Brit J Cancer 51: 543-549 https://doi.org/10.1038/bjc.1985.77
  13. Epstein JH. 1983. Photocarcinogenesis, skin cancer and aging. Journal of the American Academy of Dermatology 9: 487-502 https://doi.org/10.1016/S0190-9622(83)70160-X
  14. Bolognia JL, Berwick M, Fine JA. 1990. Complete follow- up and evaluation of skin cancer screening in Connecticut. Journal of the American Academy of Dermatology 23: 1098- 1106 https://doi.org/10.1016/0190-9622(90)70340-N
  15. Oliver CN, Ahn B, Moerman EJ, Goldstein S, Stadtman ER. 1987. Age-related changes in oxidized proteins. J Biol Chem 262: 5488-5491
  16. Livine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. 1990. Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology 186: 464-478 https://doi.org/10.1016/0076-6879(90)86141-H
  17. Huachen W, Xueshu Z, Yan W, Mark L. 2002. Inhibition of ultraviolet light-induced oxidative events in the skin and internal organs of hairless mice by isoflavone genistein. Cancer Letters 185: 21-29 https://doi.org/10.1016/S0304-3835(02)00240-9
  18. Yoshimasa N, Akira M, Koichi K, Hajime O. 1996. Inhibitory effect of pheophorbide a, a chlorophyll-related compound, on skin tumor promotion in ICR mice. Cancer Letters 108: 247-255 https://doi.org/10.1016/S0304-3835(96)04422-9
  19. Mc Cord JM, Fridovich I. 1969. Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J Biol Chem 244: 6049-6055
  20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein determination with the Folin phenol reagent. J Biol Chem 193: 265-275
  21. Reddy GK, Enwemeka CS. 1996. A simplified method for the analysis of hydroxyproline in biological tissues. Clinical Biochemistry 29: 225-229 https://doi.org/10.1016/0009-9120(96)00003-6
  22. Davies KJ, Goldberg AL. 1987. Protein damaged by oxygen radicals are rapidly degraded in extracts of red blood cells. J Biol Chem 262: 8227-8234
  23. Agarwal S, Sohal RS. 1996. Relationship between susceptibility to protein oxidation, aging, and maximum life span potential of different species. Experimental Gerontology 31: 365-372 https://doi.org/10.1016/0531-5565(95)02039-X
  24. Harabin AL, Braisted JC, Flynn ET. 1990. Response of antioxidant enzymes to intermittent and continuous hyperbaric oxygen. Journal of Applied Physiology (Bethesda, Md.: 1985) 69: 328-335
  25. Perze R, Lopez M, Barja de Quiroga G. 1991. Aging and lung antioxidant enzyme, glutathione and lipid peroxidation in the rat. Free Rad Biol Med 10: 35-39 https://doi.org/10.1016/0891-5849(91)90019-Y
  26. Chandan KS. 1997. Nutritional biochemistry of cellular glutathione. J Nutr Biochem 8: 660- 672 https://doi.org/10.1016/S0955-2863(97)00113-7
  27. Farooqui MY, Day WW, Zamorano DM. 1987. Glutathione and lipid peroxidation in the aging rat. Comp. Comparative Biochemistry and Physiology 88: 177-180 https://doi.org/10.1016/0305-0491(87)90097-6
  28. Cho WG. 1992. The role of reactive oxygen species on UV- A induced aging of dermal collagen. MS Thesis. Chungbuk University
  29. Ranjit C, Mara J, Benzinger Jayne L, Ritter, Donald L, Bissette. 1990. Chronic ultraviolet B radiation-induced biocemical changes in the skin of hairless mice. Photochem Photobiol 51: 91-97 https://doi.org/10.1111/j.1751-1097.1990.tb01688.x
  30. Kim MH. 1998. The effect of ascorbic acid on the enzyme reaction in pyridinoline formation during soluble collagen maturation. J Korean Soc Food Sci Nutr 27: 305-312
  31. Robinson JK. 1990. Behavior modification obtained by sun protection education coupled with removal of a skin cancer. Archives of Dermatology 126: 477-481 https://doi.org/10.1001/archderm.126.4.477
  32. Mathews-Roth MM, Krinsky NI. 1985. Protein carbonyls carotenoid dose level and protection against UV-B induced skin tumors. Photochem Photobiol 42: 35-38 https://doi.org/10.1111/j.1751-1097.1985.tb03544.x

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