Screening Biological Activities of Grape Seed and Skin Extracts of Campbell Early (Vitis labruscana B.)

  • Park, Sung-Jin (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Park, Boo-Kil (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Oh, Deog-Hwan (School of Biotechnology and Bioengineering, Kangwon National University)
  • Published : 2002.09.01


This study was conducted to determine biological activities, such as lipid peroxidation inhibition, cytotoxicity, sun blocker, inhibition of tyrosinase, and antioxidative effect, of ethanol extracts, and of solvent fractionated ethanol extracts obtained from grape seeds and skins. The strongest lipid oxidative inhibition of 66.9% and 67.6% was observed respectively, in the presence of 20 $\mu\textrm{g}$/$m\ell$ of both ethanol extract and water fraction of grape seeds. Overall, the ethanol extracts and their fractions of grape seeds exhibited stronger lipid oxidative inhibition than that of skin extracts. On the other hand, the ethanol extracts of grape skins showed stronger cytotoxicity than that of seeds on MCF-7, Hep3B, and A549 cancer cell lines. However, the water fraction of seed ethanol extracts showed the strongest cytotoxic effect of 76.52% and 67.01% on MCF-7 and Hep3B, respectively among their fractions. Ethanol seed extracts obtained at 3$0^{\circ}C$ had the strongest absorbance both at UVA region (350 nm) and UVB region (308 nm) and the chloroform fraction showed the strongest absorbance at W region and butanol fraction at UVA region among their tractions, respectively. In the meantime, the ethanol extracts obtained at 3$0^{\circ}C$ and butanol fraction showed the strongest tyrosinase inhibitory effect of 39.4% and 37.6%, respectively. This study shows that ethanol extracts and their fractions of grape seeds and skins could be potential good materials for functional food and cosmetic products.


  1. Manzocco L, Anese M, Nicoli MC. 1998. Antioxidant properties of tea extracts as affected by processing. Lebensm- Wiss U Technol 31: 694-698
  2. Wang JN, Chen YJ, Hano Y, Nomura T, Tan RX. 2000. Antioxidant activity of polyphenols from seeds of Vitis amurensis in vitro. Acta Pharmacol Sin 21: 633-636
  3. Cho SY, Han YB, Shin KH. 2001. Screening for antioxidant activity of edible plants. J Korean Soc Food Sci Nutr 30: 133-137
  4. Chung HS. 2001. Isolation of new bioactive phytochemicals from natural products. Food Ind Nutr 6: 53-59
  5. Peterson DM. 2001. Oat antioxidants. J Cereal Sci 33: 115- 129
  6. Halliwell B. 1996. Antioxidants in human health and disease. Annual Review of Nutrition 16: 33-50
  7. Wang JF, Schramm DD, Holt RR, Ensunsa JL, Fraga CG, Schmitz HH, Keen CL. 2000. A dose-response effect from chocolate consumption on plasma epicatechin and oxidative damage. J Nutr 130: 2115S-2119S
  8. Lee MC, Kim GP, Kim SH, Choung NH, Yim MH. 1997. Antimicrobial activity of extract from gall-nut and red-grape husk. Korean J Food and Nutr 10: 174-179
  9. Hur SK, Kim SS, Heo YH, Ahn SM, Lee BG, Lee SK. 2001. Effects of the grapevine shoot extract on free radical scavenging activity and inhibition of pro-inflammatory mediator production. J Applied Pharmacology 9: 188-193
  10. Shirataki Y, Kawase M, Saito S, Kurihara T, Tanaka W, Satoh K, Sakagami H, Motohashi N. 2000. Selective cytotoxic activity of grape peel and seed extracts against oral tumor cell lines. Anticancer Res 20: 423-426
  11. Sato M, Maulik G, Ray PS, Bagchi D, Das DK. 1999. Cardioprotective effects of grape seed proanthocyanidin against ischemic reperfusion injury. J Mol Cell Cardiol 31: 1289- 1297
  12. Zhao J, Wang J, Chen Y, Agarwal R. 1999. Anti-tumor- promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent. Carcinogenesis 20: 1737-1745
  13. Saucier C, Mirabel M, Daviaud F, Longieras A, Glories Y. 2001. Rapid fractionation of grape seed proanthocyanidins. J Agric Food Chem 49: 5732-5735
  14. Kim SK, Kim YG, Lee MK, Han JS, Lee JH, Lee HY. 2000. Comparison of biological activity according to extracting solvents of four Acanthopanax root Bark. J Medicinal Crop Sci 8: 21-28
  15. Jeong KJ, Sa JH, Rhu MJ, Kim JB, Lee W, Han KS, Oh HS, Choi KY, Park SK and Cheung EH. 2000. Screening on sunsreen agents from domestic plants grown in the area of Kangwon province. Rep Inst Health and Environ 11: 69- 83
  16. Jung SW, Lee NK, Kim SJ, Han DS. 1995. Screening of tyrosinase inhibitor from plants Korean J Food Sci Technol 27: 891-896
  17. Carnesecchi S, Schneider Y, Lazarus AS, Coehlo D, Gosse F, Raul F. 2002. Flavanols and procyanidins of cocoa and chocolate inhibit growth and polyamine biosynthesis of human colonic cancer cells. Cancer Letters 175: 147-155
  18. Singletary KW, Meline B. 2001. Effect of grape seed proanthocyanidins on colon aberrant crypts and breast tumors in a rat dual-organ tumor model. Nutr Cancer 39: 252-258
  19. Choi S, Kim H, Chang E, Sapers GM. 1997. Inhibition of tyrosinase activity by plant extracts. Foods and Biotechnology 6: 44-49

Cited by

  1. Free Radical Scavenging Effect of Seed and Skin Extracts from Campbell Early Grape (Vitis labruscana B.) vol.32, pp.1, 2003,
  2. Detection of Radiation Induced Markers in Oranges Imported from the United States of America vol.32, pp.1, 2003,
  3. Effect of Roasting Conditions on the Quality of Grape Seed Tea vol.18, pp.1, 2014,