The Effects of Isoflavone on Lipid Metabolism and Immune Responses in SD Rats

Isoflavone 섭취가 흰쥐의 지방대사 및 면역능력에 미치는 영향

  • 정미경 (단국대학교 식품영양학과) ;
  • 방명희 (단국대학교 식품영양학과) ;
  • 설소미 (단국대학교 식품영양학과) ;
  • 김우경 (단국대학교 식품영양학과)
  • Published : 2002.07.01


This study was performed to investigate the effects of isoflavone consumption on plasma and liver lipid profiles and immune responses in Sprague-Dawley male rats. Experimental animals fed isoflavone at various doses for 4 weeks (0, 1095, 2190, 4380 isoflavone mg/kg diet). Exposure to isoflavone decreased the food consumption and final body weights of rats without decreasing the relative weights of organs, hemoglobin and hematocrit. And the plasma cholesterol and LDL-cholesterol, liver total lipid, cholesterol and triglyceride concentrations were significantly decreased by isoflavone intakes. The absolute and relative weights of thymus were significantly decreased in groups fed isoflavone than in control. Also splenocyte proliferations with Con A or PHA were decreased according to isoflavone consumption in rats, although there was not significant. These results demonstrate that isoflavone intakes significantly improve lipid profiles in plasma and liver. But the effects of isoflavone intakes on immune responses are needed further experiments.


  1. Cancer Causes Control v.6 Phytoestrogens, body composition,and breast cancer Horn-Ross PL.
  2. Biochem Pharmacol v.24 Vasodilatory effects of flavonoids in rat aortic smooth muscle, structure activity relationships Duarte J;Vizcaino FP;Utrilla P;Jimenes J;Zarauelo A
  3. Planta Med v.67 no.8 Osteoblastic proliferation stimulating activity of Psoralea corylifolia extracts and two of its flavonoids Wang D;Li F;Jiang Z
  4. Endocrinology v.140 no.4 Selective effects of genistein,a soybean isoflavone, on B-lymphopoiesis and Bone loss caused by estrogen deficiency Ishma Y;Miyaura C;Ohmura M;Onoe Y;Sato T;Uchiyama;Ito M;Wang X;Suda T;Ikegami S
  5. Free Radical Biol Med v.16 The correlation between active oxygens scavenging and antioxidative effects of flavonoids Hanasaki Y;Ogawa A;Fukui S
  6. Biochem Parmacol v.36 Flavonoids as antioxidants evaluated by in vitro and in situ liver chemiliminescence Fraga CG;Martino VS;Ferraro GE;Coussio JD;Boveris A
  7. Phytochemistry v.25 Antioxidants activity of flavonoids and reactivity with peroxy radical Torel J;Cillard J;Cillard P
  8. Eur Urol v.35 Diet and its preventive role in prostatic disease Denis L;Morton MS;Griffiths K
  9. Morton MS. Nutrition and Cancer Griffths K;Adlercreutz H;Boyle P;Denis L;Nicholson RI
  10. Report on 1998 national health and nutrition survey Ministry of health and welfare
  11. Nutrition and Cancer v.33 no.2 Dietary intake and sources of isoflavones among Japanese Wakai K;Egami I;Kato K;Kawamura T;Tamakoshi A;Lin Y;Nakayama T;Wada M;Ohno Y
  12. Kor J Comm Nutr v.5 no.1 A study of dietary isofalvone intake from soy foods and urinary isofalvone excretion and menopausal symptons in Korean women in rural areas Suhg CJ;Choi SH;Kim MH;Park MH;Ko BS;Kim HK
  13. Lancet v.337 Day NE. Dietary effects on breast-cancer risk in Singapore Lee HP;Gourley L;Duffy SW;Esteve J
  14. Jpn J Cancer Res v.86 A large-scale, hospital-based case-control study of risk factors of breast cancers according to menopausal status Hirose K;Tajima K;Hamajima N
  15. Biochem Pharm v.60 Dietary soy-derived isoflavone phytoestrogens Tikkanen MJ;Adlercreutz H
  16. J Nutr v.130 Isofalvone aglycone-rich extract without soy protein attenuates atherosclerosis development in cholesterolfed rabbits Yamakoshi J;Piskula MK;Izumi T;Tobe K;Saito M;Kataoka S;Obata A;Kikuchi M
  17. Cancer Res v.58 Estrogenic effects of genistein on the growth of estrogen receptor-positive human breast cancer(MCF-7) cells in vitro and in vivo Hsieh CY;Santell RC;Haslam SZ;Helferich WG
  18. J Nut v.123 AIN-93 Purified Diets for Laboratory Rodents:Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet Reeves PG;Nielsen FH;Fahey GC
  19. Clin Chem v.18 Estimation of the concentration of low density lipoprotein cholesterol in plasma,without use of the preparative ultracentrifugation Friedwald WT;Levy RI;Fredrickson DS
  20. J Biochem v.223 A simple method for the isolation and purification of total lipids from animal tissues Folch JM;Lees G;Stanley HS
  21. Standard method chemistry Total and free cholesterol Zak B
  22. J Nutr v.130 Mass pectrometric determination of genistein tissue distribution in diet-exposed sprague-dawley rats Chang HC;Churchwell MI;Delclos KB;Newbold RR;Doerge DR
  23. Reproduc Toxicol v.15 Reproductive effects in male and female rats of neonatal exposure to genistein Nagago T;Yoshimura S;Saito Y;Nakagomi M;Usumi K;Ono H
  24. J Endocrinol v.170 Dietary soy-phytoestrogens decrease testosterone levels and prostate weight without altering LH, prostate 5 alpha-reductase or testicular steroidogenic acute regulatory peptide levels in adult male Sprague-Dawley rats Weber KS;Setchell KD;Stocco DM;Lephart ED
  25. Reproduc Toxico v.15 Newbold RR. Effects of dietary genistein exposure development on male and female CD(Sprague-Dawley)rats Delcols KB;Bucci TJ;Lomax LG;Latendresse JR;Warbritton A;Weis CC
  26. Poult Sci v.80 Dietary effects od soy isoflavone on growth and cacass traits of commercial broiler Payne RL;Binder TD;Southern LL;Mcmillin KW
  27. Estrogens in the environment Ⅱ:Influences on development Naturally occurring non-steroidal estrogens of dietary origin Setchell KDR;MaLachlan JA(Ed.)
  28. Am J Clin Nutr v.71 Soy isoflavones improve plasma lipids in normocholesterolemic, premenopausal women Merz-Demlow BE;Duncan AM;Wangen KE;Xu X;Carr TP;Phipps WR;Kurzer MS
  29. Am J Clin Nutr v.72 Isofalvone phytoestrogens consumed in soy decrease F2-isoprostane concentrations and increase resistance of low density lipoprotein to oxidation in human Wiseman H;Lewis P;Wajala K;Adlercreutz H;Mallet AI;Bowey EA;Rowland IR;Sanders TA
  30. Am J Clin Nutr v.68 Effects of soy isoflavones on atherosclerosis:Potential mechanism Anthony MS;Clarkson TB;Williams JK
  31. Fertil Steril v.67 Soy isoflavones enhance coronary vascular reactivity in atherosclerotic female macaques Honore EK;Williams JK;Anthony MS;Clarkson TB
  32. J Nutr v.125 Biology of atherosclerotic plaque formation:Possible role of growth factors in lesion development and the potential impact of soy Raines EW;Ross R
  33. J Boil Chem v.262 Genistein,a specific inhibitor of tyrosine-specific protein kinase Akiyama T;Ishida J;Nakagawa S;Ogawara H;Watanabe S;Itoh N;Shibuya M;Fukami Y
  34. J Nutr v.131 Genistein modulates immune responses and increases host resistance to B16F10 tumor in adult female B6C3F1 mice Santell RC;Kieu N;Helferich WG
  35. J Nutr v.130 Genistein inhibits growth of estrogen-independent human breast cancer cells in culture but not in athymic mice Santell RC;Kieu N;Helferich WG
  36. J Investing Allergol Clin Immunol v.5 Protein tyrosine kinase inhibition and cell proliferation:is the [3H]-thymidine uptake assay representative of the T-lymphocyte proliferation rate? Spinozzi F;Pagliacci MC;Agea E;Migliorati G;Riccardi C;Bertotto A;Nicoletti I
  37. Int J Immunopharmacol v.21 Inhibitory effect of natural and environmental estrogens on thymic hormone production in thymus epithelial cell culture Sakabe K;Okuma M;Karaki S;Matsuura S;Yoshida T;Aikawa H;Izumi S;Kayama F
  38. Mol Immunol v.35 Concanavalin A modulates tyrosine phosphorylation and activation of a type Ⅱ phosphatidylinositol 4-kinase in rat splenic lymphocytes Fernandis AZ;Subrahmanyam G
  39. Immunology v.80 Requirement for tyrosine phosphorylation in lipopolysaccharide-induced murine B-cell proliferation Dearden-Badet MT;Revillard JP
  40. Nutr Cancer v.29 Enhancement of immune function in mice fed high doses of soy daidzein Zhang R;Li Y;Wang W