PCR-RFLP Analysis of Ribosomal DNA Intergenic Spacer Region in Fusarium section Liseola.

Fusarium section Liseola 균주들에서 rDNA Intergenic Spacer 부위의 PCR-RFLP 분석

  • 이경은 (상명대학교 자연과학부 생명과학과) ;
  • 최영길 (한양대학교 자연과학부 생명과학과) ;
  • 민병례 (상명대학교 자연과학부 생명과학과)
  • Published : 2002.03.01


The intergenic spacer (IGS) region of the ribosomal DNA of species in Fusarium section Liseola was analyzed by amplification and subsequent digestion with several restriction enzymes. The length of the amplified IGS region was about 2.6 Kb in all strains except F.moniliforme 12 Which was about 2.9 Kb. The enzymes, EcoRI, HincII, SalI, HindIII, PstI and SmaI, digested the IGS region and nine haplotypes were identified among 11 strains. In the dendrogram based on PCR-RFLP of IGS region combined the results of section Liseola in this study and section Elegans in previous study, variation in the IGS appears to offer considerable potential to resolve intraspecific relationship as well as interspecies or intersection.


Fusarium;haplotype;IGS;PCR-RFLP;restriction enzyme


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