Isolation and Characterization of $\alpha$-Amylase Producing Bacillus sp. AIV 1940 and Properties of Starch Synthetic Wastewater Degradation

$\alpha$-Amylase 생성균주 Bacillus sp. AIV 1940의 분리, 특성 및 합성폐수분해능

  • 박형수 (삼성엔지니어링 기술연구소) ;
  • 김무훈 (삼성엔지니어링 기술연구소) ;
  • 양선영 (삼성엔지니어링 기술연구소) ;
  • 조미영 (삼성엔지니어링 기술연구소) ;
  • 고범준 (삼성엔지니어링 기술연구소) ;
  • 박용근 (고려대학교 생명공학원)
  • Published : 2002.03.01

Abstract

$\alpha$-Amylase producing bacteria were isolated from activated sludge of corn processing wastewater plant and paddy field soil samples and selected by the direct iodine reaction. The isolate was identified as Bacillus sp. after morphology, API system and fatty acid analyses. To enchance $\alpha$-amylase productivity, a successive mutation of Bacillus sp. AIV 19 was performed using the treatment of nitrosoguanidine(NTG).The mutant, Bacillus sp. AIV 1940, showed about 1.8-fold level of amylase activity compared with parental strain. The isolate was Gram-positive and rod (2.8-3.0 $\mu$m long, 0.5-0.6 $\mu$m wide) type. The strain increased the bacterial mass at 3000 mg/l starch concentration. Organic substance removal rate was 40.2, 72.3% respectively after 1 and 3 day reaction using starch synthetic wastewater (intial CODcr was 4,455 mg/l).

Keywords

$\alpha$-amylase;Bacillus sp.;starch degradation

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