Induction of laccases under acidic stresses in several mushroom-forming fungi.

버섯균류에서 산충격에 의한 Laccase의 유도

  • 김근숙 (강원대학교 자연과학대학 생명과학부 미생물생리학 실험실) ;
  • 금잔디 (강원대학교 자연과학대학 생명과학부 미생물생리학 실험실) ;
  • 최형태 (강원대학교 자연과학대학 생명과학부 미생물생리학 실험실)
  • Published : 2002.03.01


Induction of laccase isozymes under acidic stresses were determined in Trametes versicolor, Pleurotus ostreatus and Ganoderma lucidum isolated in Korea, and in Lentinus squarrosulrs isolated in Thai. When cultures of these fungi were transferred to acidic liquid media (pH 3.0-4.0), the activities of secreted extralcellular laccases were increased 60% and 400% in T. versicolor and G. lucidum respectively. However, there was no such induction in L. squarrosulus or P. ostreatus. In L. squarrosulus, different laccase isozymes in the electrophoretic mobilities were induced under acidic conditions.


acidic induction;laccases;medicinal mushroom


  1. Phytochem. v.29 Cucurbitacins protect cucumber tissue against infection by Botrytis cinerea Bar-Nun, N.;A.M. Mayer
  2. Appl. Microbiol. Biotechnol. v.54 Enzymology of Phanerochaete chrysosporium with respect to the degradation of recalcitrant compounds and xenobiotics Cameron, M.D.;S. Timofeevsky;S.D. Aust
  3. FEMS Microbiol. Lett. v.172 Transformation and laccase mutant isolation in Coprinus congregatus by restriction enzyme-mediated integration Leem, Y.;S. Kim;I.K. Ross;H.T. Choi
  4. J. Gen. Microbiol. v.117 Inactivation of extracellular laccase during fruiting of Agaricus bisporus Wood, D.A.
  5. 미생물학회지 v.33 Coprinus congregatus에서 산성액체배지에서의 laccase역할 김순자;임영은;최형태
  6. FEMS Microbiol. Lett. v.195 Cloning of an acidic laccase gene (clac2) from Coprinus congregatus and its expression by external pH Kim, S.;Y. Leem;K. Kim;H.T. Choi
  7. Infec. Immun. v.67 Laccase protects Cryptococcus neoformans from antifungal activity of alveolar macrophages Liu, L.;R.P. Tewari;P.R. Williamson
  8. J. Basic Micorbiol v.38 Degradation of 2-chlorophenol and formation of 2-chloro-1,4-benzoquinone by mycelia and cell-free culture liquids of Trametes versicolor in relation ot extracellular laccase activity Grey, R.;C. Hofer;D. Schlosser<371::AID-JOBM371>3.0.CO;2-V
  9. Appl. Microbiol. Biotechnol. v.57 Purification and characterization of laccase isozymes from the white-rot basidiomycete Ganoderma lucidum Ko, E.M.;Y.E. Leem;H.T. Choi
  10. Eur. J. Biochem. v.269 Oxidation of aromatic sulfides by lignin peroxidase from Phanerochaete chrysosporium Baciocchi, E.;M.F. Gerini;P.J. Harvey;O. Lanzalunga;S. Mancinelli
  11. J. Bacteriol. v.173 Regulation of lacoase biosynthesis in the plant-pathogenic fungus Cryphonectria parasitica by double-stranded RNA Rigling, D.;N.K. Van Alfen
  12. Mycol. v.79 Formation of sclerotia in liquid cultures of Coprinus congregatus and their phenoloxidase isozymes Choi, H.T.;R.L. Wilks;I.K. Ross
  13. Crit. Rev. Immunol. v.19 Therapeutic effects of substances occurring in higher basidiomycetes mushrooms: a modern perspective Wasser, S.P.;A.L. Weis
  14. Phytochem. v.49 Anti-HIV-1 and Anti-HIV-1-protease substances from Ganoderma lucidum el-Mekkawy, S.;M.R. Meselhy;N. Nakamura;Y. Tezyka;M. Hattori;N. Kakiuchi;K. Shimotohno;T. Kawahata;T. Otake