Optimal Condition for Mycelial Growth of Beauveria bassiana and Its Extracellular Enzyme Activity

백강균(Beauveria bassiana)의 균사체 최적 배양조건 및 효소활성

  • 민응기 (신화제약(주)) ;
  • 한영환 (동국대학교 자연과학대학 생물학과)
  • Published : 2002.03.01


The optimum temperature and pH for mycelial growth of B. bassiana DGUM 34001 were $24^{\circ}C$ and pH 7.0, respectively. Among the complex media used, mushroom complex medium (MCM) was the most favorable for mycelial growth. When Czapek-Dox medium was used as a minimal medium, glucose was an excellent source for carbon and energy. Soytone and sodium phosphate were favorable constituent for culture medium as a source of organic nitrogen and phosphorus, respectively. When the fungus was grown in MCM broth, the specific activity of extracellular enzyme of ${\alpha}$-amylase, lipase, chitinase, CMCase and pretease were 297.0, 0.058, 0.33, 0.21 and 22.8 units/mg protein, respectively. When various sources of organic nitrogen and chitin were supplemented to determine the production of enzymes, casein and soluble chitosan enhanced the production of extracellular protease and chitinase.


Beauveria bassiana;enzyme activity;mycelial growth;stimulation


  1. J. Gen. Microbiol. v.135 Ultrastructural localization of a cuticle-degrading protease produced by the entomophathogenic fungus Metarhizium anisopliae during penetration of host (Manduca sexta) cuticle Goettel, M.S.;R.J. Leger;N.W. Rizzo;R.C. Staple;D.W. Roverts
  2. Proc. Biochem. v.34 Impact of process parameters on chitinase production by an alkalophilic marine Beauveria bassiana in solid state fermentation Suresh, P.V.;M. Chandrasekaran https://doi.org/10.1016/S0032-9592(98)00092-2
  3. J. Inverter. Pathol. v.17 Toxins of the entomophagous fungus Beauveria bassiana Ⅱ. Effect of nitrogen sources ofn formation of the toxic protease in submerged culture Kucera, M. https://doi.org/10.1016/0022-2011(71)90093-0
  4. 한국균학회지 v.26 송이균(Tricholoma matsutake) 배양액의 세포외 효소 활성 이창윤;홍운표;정명준;한영환
  5. Toxicol. v.35 Toxic properties of Beauveria bassiana pigments on erythrocyte membranes Jeffs, L.B.;G.K. George
  6. 미생물학회지 v.36 녹강균(Metarhizium anisopliae)의 최적 배양조건 및 효소활성 박영일;한영환
  7. J. Inverteb. Pathol. v.41 Growth and sporulation of Beauveria bassiana and Metarhizium anisopliae in a basal medium containing various carbohydrate sources Campbell R.K.;G.L. Barnes;B.O. Cartweright;R.D. Elkenbary https://doi.org/10.1016/0022-2011(83)90242-2
  8. Can. J. Bot. v.73 Comparative analysis of the in vivo and in vitro metabolites produced by the entomopathogen Beauveria bassiana Boucias, D.G.;I. Mazet;J. Pendland;S.Y. Hung https://doi.org/10.1139/b95-363
  9. Indian J. Exp. Biol. v.30 Effect of temperature, pH and light on toxin production by Beauveria bassiana Sumita, S.;G.P. Agarwal;R.C. Rajak
  10. 한국균학회지 곤충병원성 진균 Beauveria속의 동정 및 계통분석에 관한 연구 김기영;이재동;민응기;한영환
  11. Appl. Env. Microbiol. v.53 Purification and properties of an extracellular protease produced by the entomopathogenic fungus Beauveria bassiana Bidochka, M.J.;G.G. Khachatourians
  12. 한국약용버섯도감 박완희;이호득