Optimal Condition for Mycelial Growth of Beauveria bassiana and Its Extracellular Enzyme Activity

백강균(Beauveria bassiana)의 균사체 최적 배양조건 및 효소활성

  • 민응기 (신화제약(주)) ;
  • 한영환 (동국대학교 자연과학대학 생물학과)
  • Published : 2002.03.01

Abstract

The optimum temperature and pH for mycelial growth of B. bassiana DGUM 34001 were $24^{\circ}C$ and pH 7.0, respectively. Among the complex media used, mushroom complex medium (MCM) was the most favorable for mycelial growth. When Czapek-Dox medium was used as a minimal medium, glucose was an excellent source for carbon and energy. Soytone and sodium phosphate were favorable constituent for culture medium as a source of organic nitrogen and phosphorus, respectively. When the fungus was grown in MCM broth, the specific activity of extracellular enzyme of ${\alpha}$-amylase, lipase, chitinase, CMCase and pretease were 297.0, 0.058, 0.33, 0.21 and 22.8 units/mg protein, respectively. When various sources of organic nitrogen and chitin were supplemented to determine the production of enzymes, casein and soluble chitosan enhanced the production of extracellular protease and chitinase.

Keywords

Beauveria bassiana;enzyme activity;mycelial growth;stimulation

References

  1. J. Gen. Microbiol. v.135 Ultrastructural localization of a cuticle-degrading protease produced by the entomophathogenic fungus Metarhizium anisopliae during penetration of host (Manduca sexta) cuticle Goettel, M.S.;R.J. Leger;N.W. Rizzo;R.C. Staple;D.W. Roverts
  2. Proc. Biochem. v.34 Impact of process parameters on chitinase production by an alkalophilic marine Beauveria bassiana in solid state fermentation Suresh, P.V.;M. Chandrasekaran https://doi.org/10.1016/S0032-9592(98)00092-2
  3. J. Inverter. Pathol. v.17 Toxins of the entomophagous fungus Beauveria bassiana Ⅱ. Effect of nitrogen sources ofn formation of the toxic protease in submerged culture Kucera, M. https://doi.org/10.1016/0022-2011(71)90093-0
  4. 한국균학회지 v.26 송이균(Tricholoma matsutake) 배양액의 세포외 효소 활성 이창윤;홍운표;정명준;한영환
  5. Toxicol. v.35 Toxic properties of Beauveria bassiana pigments on erythrocyte membranes Jeffs, L.B.;G.K. George
  6. 미생물학회지 v.36 녹강균(Metarhizium anisopliae)의 최적 배양조건 및 효소활성 박영일;한영환
  7. J. Inverteb. Pathol. v.41 Growth and sporulation of Beauveria bassiana and Metarhizium anisopliae in a basal medium containing various carbohydrate sources Campbell R.K.;G.L. Barnes;B.O. Cartweright;R.D. Elkenbary https://doi.org/10.1016/0022-2011(83)90242-2
  8. Can. J. Bot. v.73 Comparative analysis of the in vivo and in vitro metabolites produced by the entomopathogen Beauveria bassiana Boucias, D.G.;I. Mazet;J. Pendland;S.Y. Hung https://doi.org/10.1139/b95-363
  9. Indian J. Exp. Biol. v.30 Effect of temperature, pH and light on toxin production by Beauveria bassiana Sumita, S.;G.P. Agarwal;R.C. Rajak
  10. 한국균학회지 곤충병원성 진균 Beauveria속의 동정 및 계통분석에 관한 연구 김기영;이재동;민응기;한영환
  11. Appl. Env. Microbiol. v.53 Purification and properties of an extracellular protease produced by the entomopathogenic fungus Beauveria bassiana Bidochka, M.J.;G.G. Khachatourians
  12. 한국약용버섯도감 박완희;이호득