Treatment of Microencapsulated ${\beta}$- Galactosidase with Ozone : Effect on Enzyme and Microorganism

  • Kwak, H.S. (Department of Food Science and Technology, Sejong University) ;
  • Lee, J.B. (Department of Food Science and Technology, Sejong University) ;
  • Ahn, J. (Department of Food Science and Technology, Sejong University)
  • Received : 2001.10.11
  • Accepted : 2001.12.04
  • Published : 2002.04.01


The present study was designed to examine the effect of ozone treatment in microencapsulated ${\beta}$-galactosidase on inactivation of the enzyme and sterilization of microorganism. The efficiency was the highest as 78.4% when the ratio of polyglycerol monostearate (PGMS) was 15:1. Activities of lactase remaining outside the capsule were affected by ozone treatment. With the increase of ozone concentration and duration of ozone treatment, the activity reduced significantly. In sensory aspect, with 2% microcapsule addition, no significant difference in sweetness was found compared with a market milk during 12 d storage. Above result indicated that the additional washing process of lactase was not necessary to inactivate the residual enzyme. In a subsequent study, the vegetative cells of microorganisms were completely killed with 10 ppm for 10 min treatment by ozone. The present study provides evidence that ozone treatment can be used as an inactivation and a sterilization process. In addition, these results suggest that acceptable milk products containing lactase microcapsules made by PGMS can be prepared with ozone treatment.


Supported by : Anigen Co. Ltd.


  1. Bersen'eva, E. A., A. A. Inanov, T. P. Sansonova, E. M. Chernova and N. I. Oragvelidze. 1990. Microencapsulated aromatizers for tea. Pishchevaya Paomyshlennost, USSR 1:57-59.
  2. Kim, C. H., W. Lee, S. C. Baick, H. S. Kwak and J. O. Kang. 1996. Studies on the microencapsulation of $\omega$-3 polyunsaturated fatty acid. Korean J. Food Sci. Technol. 28:743-749.
  3. Kwak, H. S., M. R. Ihm and J. Ahn. 2001. Microcapsulation of $\beta$-galactosidase with fatty acid esters. J. Dairy Sci. 84:1576-1582.
  4. Sankarikutty, B. M., M. Sreekumar, C. S. Nrayanan and A. G. Mathew. 1988. Studies on microcapsulation of cardamon oil by spray drying technique. J. Food Sci. Technol., India. 25:352-356.
  5. Solomon, N. W., A. M. Guerro, and B. Torun. 1985. Dietary manipulation of postprandial colonic lactose fermentation. II. Addition of exogenous microbial $\beta$-galactosidase at meal time. Am. J. Clin. Nutr. 41:209-211.
  6. Yang, P. P. W. and T. C. Chen. 1979. Stability of ozone and its germicidal properties on poultry meat microorganisms in liquid phase. J. Food Sci. 44:501-504.
  7. Jackson, L. S. and K. Lee. 1991. Microencapsulated iron food fortification. J. Food Sci. 56:1047-1050.
  8. Onwulata, C. I., D. R. Rao and P. Vankineni. 1989. Relative efficiency of yogurt, sweet acidophilus milk, hydrolyzedlactose milk and a commercial tablet in alleviating lactose maldigestion. Am. J. Clin. Nutr. 49:1233-1237.
  9. Magee, E. L. Jr. and N. F. Olson. 1981. Microencapsulation of cheese ripening systems: Stability of microcapsules. J. Dairy Sci. 64:611-615.
  10. Shin, M. G., H. S. Kwak, P. S. Jang, B. K. Min, M. R. Yoo and D. C. Kim. 1995. Manufactures of spray solution containing microcapsulated lactase by milk fat. Korean patent. No.088465.
  11. Scrimshaw, N. S. and E. B. Murray. 1988. The acceptability of milk and milk products in populations with high prevalence of lactose intolerance. Am. J. Clin. Nutr. 48:1083-1159.
  12. Kretchmer, N. 1972. Lactose and lactase. In: Scientific American Food. (Ed. J. E. Hoff and J. Janick). Freeman Company, San Francisco. CA. USA. pp. 35-43.
  13. Mertens, B. and D. Knorr. 1992. Developments of nonthermal processes for food preservation. Food Technol. May, 124-133.
  14. Driedger, A., E. Staub, U. Pinkernell, B. Marinas, W. Koster and U. Gunten. 2001. Inactivation of Bacillus subtilis spores and formation of bromate during ozonation. Wat. Res. 35(12):2950-2960.
  15. Graham, D. M. 1997. Use of ozone for food processing. Food Technol. 51:72-75.
  16. Harris M. 1972. One man's food is another man's whitewash. Natural History 81:12-14.
  17. Simmons, F. J. 1978. The geographic hypothesis and lactose malabsoprtion: A weighing of the evidence. Digest Dis. 23:963-967.
  18. Broadwater, W. T., R. C. Hoehn and P. H. King. 1973. Sensitivity of three selected bacterial species to ozone. Appl. Microbiol. 26:391-393.
  19. Xu, L. 1999. Use of ozone to improve the safety of fresh fruits and vegetables. Food Technol. 53:58-62.
  20. SAS. 1985. User's Guide: Statistics, Version 5 Edition. SAS Institute, Inc., Cary, NC.