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Effect of Molybdenum Induced Copper Deficiency on Peripheral Blood Cells and Bone Marrow in Buffalo Calves

  • Randhawa, C.S. (Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Punjab Agricultural University) ;
  • Randhawa, S.S. (Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Punjab Agricultural University) ;
  • Sood, N.K. (Department of Veterinary Pathology, Punjab Agricultural University)
  • Received : 2001.07.05
  • Accepted : 2001.11.15
  • Published : 2002.04.01

Abstract

Copper deficiency was induced in eight male buffalo calves by adding molybdenum (30 ppm wet basis) to their diet. Copper status was monitored from the liver copper concentration and a level below 30 ppm (DM basis) was considered as deficient. Haemoglobin, haematocrit, total and differential leucocyte numbers were determined. The functions of peripheral neutrophils were assessed by in vitro phagocytosis and killing of Staphylococcus aureus. The effect of molybdenum induced copper deficiency on bone marrow was monitored. The mean total leucocyte count was unaffected whereas a significant fall in neutrophil count coincided with the fall in hepatic copper level to $23.9{\pm}2.69$ ppm. Reduced blood neutrophil numbers was not accompanied by any change in the proportion of different neutrophil precursor cells in bone marrow. It was hypothesised that buffalo calves were more tolerant to dietary molybdenum excess than cattle. It was concluded that neutropenia in molybdenum induced copper deficiency occurred without any effect on their synthesis and maturation process. Bone marrow studies in healthy calves revealed higher percentage of neutrophilic myelocytes and metamyelocytes as compared to cattle.

Keywords

Buffalo;Bone Marrow;Copper Deficiency;Molybdenum;Neutropenia

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