DOI QR코드

DOI QR Code

Detection of Retinol-binding Protein in Bovine Yolk Sac, Chorion and Allantois by Immunoperoxidase Method

  • Liu, Kaung Huei
  • Received : 2001.09.28
  • Accepted : 2001.12.29
  • Published : 2002.06.01

Abstract

Bovine yolk sac at day 24 of pregnancy, and placental membranes (chorion and allantois) from days 70 and 100 of pregnancy were isolated and cultured in a modified minimum essential medium in the presence of $[^{35}S]$methionine. Proteins synthesized and secreted by isolated bovine yolk sac, chorion and allantois were analyzed by fluorography of two-dimensional polyacrylamide gel electrophoresis. Serum-like proteins,transferrin, ${\alpha}$-fetoprotein, ${\alpha}$1-antitrypsin and ${\alpha}$1-acid glycoprotein,were the major protein products of yolk sac. A 21 kDa protein produced by yolk sac was identified immunochemically as retinol-binding protein (RBP). Chorion and allantios from days 70 and 100 of pregnancy were active in protein synthesis and secretion. Both chorion and allantois did not secret serum-like proteins but secreted a number of neutral-to-acidic proteins including RBP. Secretory proteins produced by the yolk sac, chorion and allantois may play important roles in the embryonic development and the successful outcome of pregnancy. Antiserum against bovine placental RBP was employed to the immunocytochemistry by immunoperoxidase method. Immunoreactive RBP was localized in epithelial cells and island-like cell clones of yolk sac. Immunostaining for RBP was detected in simple columnar epithelium of chorion and in simple squamous epithelium of allantois. In the present study, proteins synthesized and secreted by yolk sac at day 24 of pregnancy, chorion and allantois from days 70 and 100 of pregnancy were characterized In addition, RBP was localized in yolk sac, chorion and allantois by immunoperoxidase method. The immunoperoxidase method has been proven to be a very effective technique to identify the cellular source of protein synthesis in extraembryonic membranes.

Keywords

Bovine;Yolk Sac;Chorion;Allantois;Vitamin A

References

  1. De Mey, J. 1983. Colloidal gold probes in immunocytochemistry. In: Immunocytochrmistry, Practical Applications in Pathology and Biology (Ed. J. M. Polak and S. V. Noorden). John Wright & Sons, Bristol, pp. 82-112.
  2. Gitlin, D. and J. D. Gitlin. 1975. Fetal and neonatal development of human plasma proteins. In: The Plasma Proteins: Strucure, Function and Genetic Control (Ed. F. W. Putnum). Academic Press, New York, pp. 977-987.
  3. Hunter, R. H. F. 1985. Experimental studies of sperm transport in sheep, cows and pigs. Vet. Rec. 116-188.
  4. Lindner, G. M. and R. W. Wright. 1983. Bovine embryo mophology and evaluation. Theriogenology 20:407-416. https://doi.org/10.1016/0093-691X(83)90201-7
  5. Manabe, T., Y. Takahashi and T. Okuyama. 1987. Identification of bovine fetal and adult serum/plasma proteins by twodimensional electrophoresis and immunochemical staining. Electrophoresis 8:573-579. https://doi.org/10.1002/elps.1150081207
  6. Murthy, G. S., C. Schellenberg and H. G. Friesen. 1982. Purifications and characterization of placental lactogen. Endocrinology 111:2117-2228. https://doi.org/10.1210/endo-111-6-2117
  7. Stryer, L. 1988. Biochemistry. W. H. Freeman and Company, New York.
  8. Thaller, C. and G. Eichele. 1987. Identification and spatial distribution of retinoids in the developing chick limb bud. Nature 327:625-628. https://doi.org/10.1038/327625a0
  9. Flechon, J. E. 1978. Morphological aspects of embryonic disc at the time of its appearance in the blastocyst of farm mammals. Scanning Electron Microscopy. Vol 2, 54:548.
  10. Bongso, T. A. and P. D. Basrur. 1976. foetal fluids in cattle. Can. Vet. J. 17:38-41.
  11. Hiramatsu, M. and L. Packer. 1990. Antioxidant activity of retinoids. In: Cell Differentiation and Clinical Applications (Ed. L. Packer). Academic Press, Inc. New York. pp. 273-280.
  12. Yun, J. S., W. J. Kang, D. S. Seo, S. S. Park, K. C. Hong, C. Y. Lee and Y. Ko. 2001. Association of Endocrine factors (insulin-like growth factor-II and binding protein-3) with litter size in pigs. Asian-Aust. J. Anim. Sci. 14:307-315. https://doi.org/10.5713/ajas.2001.307
  13. Ailenberg, M. and M. Shemesh. 1983. Partial purification of a chorionic gonadotropin-like protein from bovine cotyledons. Biol. Reprod. 28:517-22. https://doi.org/10.1095/biolreprod28.3.517
  14. Chamberlain, J. P. 1979. Fluorographic detection of radioactivity in polyacrylamide gels with the water soluble fluor sodium salicylate. Anal. Biochem. 98:132-135. https://doi.org/10.1016/0003-2697(79)90716-4
  15. Greenstein, J. S., R. W. Murray and R. C. Foley. 1958. Observations on the morphogenesis and histochemistry of the bovine preattachment placenta between 16 and 33 days of gestation. Anat. Rec. 132:321-341. https://doi.org/10.1002/ar.1091320308
  16. Bazer, F. W. and N. L. First. 1983. Pregnancy and parturition. J. Anim. Sci. 57:425-46.
  17. Wolf, G. 1984. Multiple functions of vitamin A. Physiol. Rev. 64:873-937.
  18. Johnson, G. D., R. S. Davidson, K. C. McNamee, G. Russell, D. Goodwin and E. J. Holborow. 1982. Fading of immunofluorescence during microscopy: a stud y of the phenomenon and its remedy. J. Immunol. Methods 55:231-241. https://doi.org/10.1016/0022-1759(82)90035-7
  19. Gao, K., K. H. Liu and J. D. Godkin. 1991. Immunohistochemical localization of bovine placental retinol-binding protein. Int. J. Dev. Biol. 35:485-489.
  20. Liu, K. H., G. A. Baumbach, P. M. Gillevet and J. D. Godkin. 1990. Purification and characterization of bovine placental retinol-binding protein. Endocrinology 127:2696-2704. https://doi.org/10.1210/endo-127-6-2696
  21. Roberts, R. M., G. A. Baumbach, W. C. Buhi, F. B. Denny, L. A. Fitzgerald, S. Babelyn and M. N. Horst. 1984. Analysis of membrane polypeptides by two–dimensional polyacrylamide gel electrophoresis. In: Molecular and Chemical Characterization of Membrane Receptors (Ed. J. C. Ventor and L. C. Harison). Allen R. Liss, Inc., New York. pp. 61-113.
  22. Ordronneau, P., P. B. M. Lindstrom and P. Petrusz. 1981. Four unlabeled anti-body bridge techniques: a comparison. J. Histochem. Cytochem. 29:1397-404. https://doi.org/10.1177/29.12.7033366
  23. Godkin, J. D., B. J. Lifsey and G. A. Baumbach. 1988a. Characterization of bovine conceptus protein produced during the peri and postattachment periods of early pregnancy. 38:. https://doi.org/10.1095/biolreprod38.3.703
  24. Gudas, L., M. B. Sporn and A. B. Roberts. 1994. Cellular biology and biochemistry of the retinoids. In: The Retinoids: Biology, Chemistry and Medicine (Ed. M. B. Sporn, A. B. Roberrs and D. S. Goodman). New York, Raven Press Ltd. pp. 443-520.
  25. Murray, M. .K., E. C. Segerson, P. J. Hansen, F.W. Bazer and R. M. Roberts. 1987. Suppression of lymphocyte activation by a high molecular weight glycoprotein released from preimplantation ovine and porcine conceptuses. Am. J. Reprod. Immunol Microbiol 14:38-44. https://doi.org/10.1111/j.1600-0897.1987.tb00115.x
  26. Greenstein, J. S. and R. C. Foley. 1958. Early embryology of the cow. I. Gastrula and pritive streak stages. J. Dairy Sci. 41:409-421. https://doi.org/10.3168/jds.S0022-0302(58)90934-2
  27. Laemmli, U. K. 1970. Cleavage of structural proeins during the assembly of the head of bacteriophage T4. Nature 227:680-685. https://doi.org/10.1038/227680a0
  28. Knickerbocker, J. J., W. W. Thatcher, F. W. Bazer, M. Dorst, D. H. Barron, K. B. Fincher and R. M. Roberts. 1986. Proteins secreted by day 16 to 18 bovine conceptuses extend corpus luteum function in the cow. J. Reprod. Fertil. 77:381-391. https://doi.org/10.1530/jrf.0.0770381
  29. Amatruda, T. T. and H. P. Koeffler. 1986. Retinoids and cells of the hematopoietic system. In: Retinoids and Cell Differentiation (Ed. M. I. Sherman). CRC Press, Boca Raton, FL. pp. 79-103.
  30. Avivi, A., A. B. Schreiber and M. Shemesh. 1982. Isolation and characterization of a 94,000-dalton protein with thyrotropic activity from early bovine placenta. J. Biol. Chem. 257:11384-89.
  31. Sternberger, L. A., P. H. Jr. Hardy, J. J. Cuculis and H. G. Meyer. 1970. The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidaseantiperoxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18:315-333. https://doi.org/10.1177/18.5.315
  32. Godkin, J. D., B. J. Lifsey and G. A. Baumbach. 1988b. Characterization of protein production by bovine chorionic and allantonic membranes. Biol. Reprod. 39:195-204. https://doi.org/10.1095/biolreprod39.1.195
  33. Thesleff, I., A. M. Partanen, W. Lanshulz , I. S. Trowbridges and P. Ekblom. 1985. The role of transferring receptors and iron delivery in mouse embryonic morphogenesis. Differentiation 30:152-158. https://doi.org/10.1111/j.1432-0436.1985.tb00526.x
  34. May, W. S. Jr and P. Cuatrecacas. 1985. Transferrin receptor: its biological significance. J. Membr. Biol. 88:205-215. https://doi.org/10.1007/BF01871086
  35. Travis, J. and G. S. Salvesen. 1983. Human plasma proteinase inhibitors. Ann. Rev. Biochem. 52:655-709. https://doi.org/10.1146/annurev.bi.52.070183.003255
  36. Flechon, J. E. and J. P. Renard. 1978. A scanning electron microscope study of the hatching of bovine blastocyst in vitro. J. Reprod. Fertil. 53:9-12. https://doi.org/10.1530/jrf.0.0530009
  37. Massip, A., J. Mulnard, R. Huygens, C. Hanzen, P. Van der Zwalmen and F. Ectors. 1981. Ultrastructure of the cow blastocyst. J. Submicrosc. Cytol. 13:31-40.
  38. Carp, H., F. Miller, J. R. Hoidal and A. Janoff. 1982. Protential mechanism of emphysema $\alpha$1-proteinase inhibitor recovered from lungs of cigarette smokers contains oxidized methionine and has decreased elastase inhibitory capacity. Proc. Nat. Acad. Sci. 79:2041-2045. https://doi.org/10.1073/pnas.79.6.2041