The healing effect of rhGM-CSF on uninfected wounds

rhGM-CSF(Leucogen)의 비감염성 상처 치유 효과에 관한 연구

  • Han, Seung Kyu (Department of Plastic Surgery, School of Medicine, Korea University) ;
  • Kim, Byung Soo (Department of Internal Medicine, School of Medicine, Korea University) ;
  • Kim, Aeree (Department of Pathology, School of Medicine, Korea University) ;
  • Seo, Jae Hong (Department of Internal Medicine, School of Medicine, Korea University) ;
  • Choi, Chul Won (Department of Internal Medicine, School of Medicine, Korea University) ;
  • Shin, Sang Won (Department of Internal Medicine, School of Medicine, Korea University) ;
  • Kim, Yeul Hong (Department of Internal Medicine, School of Medicine, Korea University) ;
  • Kim, Woo Kyung (Department of Plastic Surgery, School of Medicine, Korea University) ;
  • Kim, Jun Suk (Department of Internal Medicine, School of Medicine, Korea University)
  • 한승규 (고려대학교 의과대학 성형외과학교실) ;
  • 김병수 (고려대학교 의과대학 내과학교실) ;
  • 김애리 (고려대학교 의과대학 내과학교병리학교실) ;
  • 서재홍 (고려대학교 의과대학 내과학교실) ;
  • 최철원 (고려대학교 의과대학 내과학교실) ;
  • 신상원 (고려대학교 의과대학 내과학교실) ;
  • 김열홍 (고려대학교 의과대학 내과학교실) ;
  • 김우경 (고려대학교 의과대학 성형외과학교실) ;
  • 김준석 (고려대학교 의과대학 내과학교실)
  • Published : 2001.04.30

Abstract

Background: rhGM-CSF has been shown to enhance the migration and proliferation of endothelial cells and to promote keratinocyte growth. This study was tried to evaluate the effect of rhGM-CSF dressing on the uninfected wounds. Methods: Thirty Sprague-dawley white mice(250-300g) were selected in this study. The number of wound with the diameter of 5 mm, was 3 in left and 3 in right at the symmetric sites, respectively. The site of rhGM-CSF dressing was decided by a randomization. rhGM-CSF($Leucogen^{(R)}$) was diluted in the distilled water($5{\mu}g/mL$). The experimental wound group was dressed by l mL of distilled water mixed with rhGM-CSF and control wound group was dressed by l mL of distilled water. The dressing was done, every 24 hours. The criteria of comparison were the duration of wound healing duration, histologic findings and the bacterial culture of wound sites. Results: The duration of wound healing was $10.3{\pm}1.7days$ in experimental group and $10.2{\pm}2.8days$ in control group, without significant difference. There was no specific difference of histologic findings between both groups. The pathogen was not found, at all. Conclusion: It seems to be that rhGM-CSF has no prominent effect on the uninfected wound healing in the mice without immune suppression.