DOI QR코드

DOI QR Code

Effect of Hydroquinone on Ruminal Urease in the Sheep and its Inhibition Kinetics in vitro

  • Zhang, Y.G. (College of Animal Science & Technology, College of Northeast Agricultural University) ;
  • Shan, A.S. (College of Animal Science & Technology, College of Northeast Agricultural University) ;
  • Bao, J. (College of Animal Science & Technology, College of Northeast Agricultural University)
  • Received : 2001.02.22
  • Accepted : 2001.05.10
  • Published : 2001.09.01

Abstract

Effect of hydroquinone (HQ) on rumen urease activity was studied. Hydroquinone at concentrations of 0.01 ppm, 0.1 ppm, 1 ppm, and 10 ppm inhibited urease activity of intact rumen microbes in vitro by 25%, 34%, 55% and 64% respectively. In the presence of low concentrations of $\beta$-mercaptoethanol, rumen urease could be solubilized and partially purified. The Km for the enzyme was $2{\times}10^{-3}$ M with Vmax of $319.4{\mu}moles/mg$ min. The kinetics of inhibition with partially purified rumen urease was investigated. The result showed that the inhibitory effect was not eliminated by increasing urea concentrations indicating a noncompetitive effect in nature with an inhibition constant $1.2{\times}10^{-5}$ M. Hydroquinone at the concentration of 10 ppm produced 64% urease inhibition, did not affect ruminal total dehydrogenase and proteolytic enzyme (p>0.05), but increased cellulase activity by 28% (p<0.05) in vitro. These results indicated that hydroquinone was a effective inhibitor of rumen urease and could effectively delay urea hydrolysis without a negative effect. The inhibitor appeared to offer a potential to improve nitrogen utilization by ruminants fed diets containing urea.

Keywords

Hydroquinone;Ruminal Urease;Inhibition Kinetics;Sheep

Cited by

  1. Insights into Abundant Rumen Ureolytic Bacterial Community Using Rumen Simulation System vol.7, pp.1664-302X, 2016, https://doi.org/10.3389/fmicb.2016.01006