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Improvement of In Vitro Development of Bovine Embryos in a Medium Containing Selenium

  • Lee, J.H. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Park, J.H. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, K.M. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Im, K.S. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Jin, D.I (Department of Applied Biological Science, College of Natural Sciences, Sun Moon University)
  • Received : 2000.09.20
  • Accepted : 2000.10.25
  • Published : 2001.02.01

Abstract

The objective of this study was to investigate the effect of selenium (Se) on in vitro development of bovine embryos. In CR1BSA, FBS-free medium, the bovine embryos could not proceed past the developmental block more efficiently to morula stage than in chemically undefined media. Addition of glutathione precursor, cysteine, with $\beta$-mercaptoethanol did not improve the development in chemically defined medium and neither did glutathione alone. Exogenous selenium improved the embryonic development to the morula and blastocyst stages at 6 days post-insemination (dpi) significantly (67.1% vs 57.5%, p<0.05), and blastocyst stage at 8 dpi (30.1% vs 20.5%, p>0.05). These improvements might be induced by elevated glutathione peroxidase activity due to addition of Se, and a possible mechanism of selenium to elevate the activity of glutathione peroxidase is discussed.

Keywords

Bovine;Embryos;Cysteine;Glutathione;Selenium;In Vitro Development

Cited by

  1. Plant protein hydrolysates (plant peptones) as substitutes for animal proteins in embryo culture medium vol.21, pp.4, 2009, https://doi.org/10.1071/RD08147