Optimization of Cholesterol Removal Conditions from Homogenized Milk by Treatment with Saponin

  • Chang, E.J. (Department of Food Science and Technology, Sejong University) ;
  • Oh, H.I. (Department of Food Science and Technology, Sejong University) ;
  • Kwak, H.S. (Department of Food Science and Technology, Sejong University)
  • Received : 2000.10.19
  • Accepted : 2001.01.09
  • Published : 2001.06.01


This study was carried out to determine the optimum conditions for cholesterol removal from homogenized milk by treatment with saponin using a response surface methodology (RSM). The effects of temperature, reaction time, and amounts of celite or saponin added on cholesterol removal from milk were investigated. The level of cholesterol removal from milk increased with saponin concentration and varied from 57.4 to 73.3%. The optimum reaction time, amount of celite addition determined by a partial differentiation of the model equation, and amount of saponin addition were 30min, 0.95% and 1.5%, respectively. Under these conditions, the predicted cholesterol removal by RSM was estimated to be 73.4%. The experimental removal value was 73.7%. Thus, there was no appreciable difference between the experimental value and the predicted value based on RSM.