Searching of Possible Target Enzymes for Herbicide Development using Commercial Plant-Specific Inhibitors

식물 특정효소저해제의 생물활성 조사에 의한 신규제초제 작용점 탐색

  • 황인택 (한국화학연구소 스크리닝 연구부) ;
  • 최정섭 (한국화학연구소 스크리닝 연구부) ;
  • 박상희 (한국화학연구소 스크리닝 연구부) ;
  • 이관휘 (한국화학연구소 스크리닝 연구부) ;
  • 이병회 (한국화학연구소 스크리닝 연구부) ;
  • 홍경식 (한국화학연구소 스크리닝 연구부) ;
  • 조광연 (한국화학연구소 스크리닝 연구부)
  • Published : 2001.03.30


This study was conducted to search new target enzymes of novel herbicide candidate. Total of 107 biochemical inhibitors reported to inhibit over than 100 different plant enzymes were purchased from commercial chemical companies. 15 inhibitors and 34 enzymes were selected by germination assay, seedling assay, wheat leaf disc assay, and whole plant assay. Among them, seven compounds of purine, phehyl-hydrazine, o-phenanthroline, oleylamine, dicyclohexylcarbodiimide, 7,8-benzoquinoline, and aminooxyacetic acid showed high herbicidal activity in the whole plant assay under greenhouse while 7,8-benzoquinone, 8-hydroxyquinoline, 2,2'-dipyridyl, and o-phenanthroline inhibited seed germination of barnyardgrass, rice, and tomato at concentrations of 1.25 to $5{\mu}M$. The compounds of 7,8-benzoquinoline, chlorpromazine, cyanuric fluoride, 4-methylpyrazole, oleylamine, tranylcypromine, and trifluoperazine inhibited the growth of cyanobacteria at 30 to $100{\mu}M$. The compounds of dicyclohexylcarbodiimide and chlorpromazine exhibited whitening effect on tile wheat leaf disc at $100{\mu}M$. These results suggest that the plant-specific enzyme inhibitors which have biological activities may supply the target enzyme for developing new herbicide candidate.