A Photosensitive Glass Chip for DNA Purification of Nucleic Acid Probe Assay

  • Kim, Joon-Ho (Department of Electrical Engineering and Computer Science(Divison of E.E), Korea Advanced Institute of Science and Technology(KAIST)) ;
  • Kim, Byung-Gyun (Department of Electrical Engineering and Computer Science(Divison of E.E), Korea Advanced Institute of Science and Technology(KAIST)) ;
  • Yoon, Jun-Bo (Department of Electrical Engineering and Computer Science(Divison of E.E), Korea Advanced Institute of Science and Technology(KAIST)) ;
  • Euisik Yoon (Department of Electrical Engineering and Computer Science(Divison of E.E), Korea Advanced Institute of Science and Technology(KAIST))
  • Published : 2001.12.01

Abstract

A new DNA purification chip is proposed and fabricated for the sample preparation of Nucleic Acid (NA) probe assay. The proposed DNA purification chip is fabricated using photosensitive glass substrate and polydimethylsiloxane (PDMS) cover fixture. We have successfully captured and eluted the DNA using the fabricated photosensitive glass chip. The fabricated DNA purification chip showed a binding capacity of $15ng/\textrm{cm}^2$and a minimum extractable input concentration of $100copies/200\muL$. The proposed DNA purification chip can be applied for low-cost, disposable sample preparation of NA probe assays.

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