Role of Proline Accumulation in Response to Toxic Copper in Microcystis aeruginosa

  • Published : 2001.12.01

Abstract

The blue green alga, Microcystis aeruginosa, was found to accumulate proline under the stressful concentration of cupric ions. The changes of proline level in Microcystis aeruginosa in response to copper(Cu) have been monitored and the function of the accumulated proline was studied with respect to its effect on Cu uptake. Exposure of Microcystis aeruginosa elevated concentrations of Cu led to accumulation of fee proline depending on the concentrations of the metal in the external medium. The greater the toxicity or accumulation of the metal, the higher the amount of proline in algal cells were found. When proline was exogenously supplied prior to Cu treatment, the absorption of Cu was markedly reduced. When exogenous proline was supplied after Cu treatment, it resulted in a remarkable desorption of the adsorbed Cu immediately after the addition of proline. Pretreatment of Microcystis aeruginosa with proline counteracted with metal-induced lipid peroxidation. The results of the present study showed a protective elect of proline on metal toxicity through inhibition of lipid peroxidation and suggested that the accumulation of proline may be related to the tolerance mechanism for dealing with Cu stress.

References

  1. J. Phycol. v.33 Adsorption and uptake of copper by the green alga Scenedesmus subspicatus(Chlorophyta) Knauer, K.;R. Behra;L. Sigg
  2. Wat. Res. v.24 Binding of cu(II) to algae in a metal buffer Xue, H. B.;L. Sigg
  3. Heavy Metal Tolerance in Plants : Evolutionary aspects Metal tolerance in eukaryotic and prokaryotic algae Reed, R. H.;G. M. Gadd;A. J. Shaw(ed.)
  4. Bot. Rev. v.57 Biochemical, physiological and structural effects of excess copper in plants Fernandes, J. C.;F. S. Henriques
  5. Bot. Mar. v.29 Growth response of Fucus vesiculosas to heavy metal, singly and in dual combinations as related to accumulation Munda, I. M.;V. Hudnik
  6. Mar. Biol. v.119 Oxidative stress induced by copper : Defence and damage in the marine planktonic diatom Ditylum brightwelli grown in continuous cultures with high and low zinc levels Rijstenbil, J. W.;J. W. M. Derksen;L. J. A. Gerringa;T. C. W. Poortvliet;A. Sandee;M. van den Berg;J. van Drie;J. A. Wijnhold
  7. Eur. J. Phycol. v.29 Some effect of copper on the dinoflagellates Amphidinium carterae and prorocentrum micans in batch culture Lage, O. M.;A. M. Parente;H. M. V. M. Soares;M. T. S. D. Vasconcelos;R. Salema
  8. J. Exp. Bot. v.36 Changes in endogenous fatty acid and lipid synthesis associated with copper pollution in Fucus spp Smith, K. L.;N. G. W. Brya;J. L. Harwood
  9. Environ. Sci. Technol. v.28 Monitoring the cellular response of Stichococcus bacillaris to exposure of several different metals using in vivo $^{31}P$ NMR and other spectroscopic techniques Zhang, W.;V. Majidi
  10. Macroalgae, Eutrophication and Trace metal Cycling in Estuaries and Lagoons, Proceedings of the COST 48 Symposium Investigations on the influence of orginic substances produced by seaweeds on the toxicity of copper Schramm, W.;J. W. Rijstenbil(ed.);S. Haritonidis(ed.)
  11. Plant, Cell and Environ. v.19 Evidence for a role for L-proline as a salinity protectant in the cyanobacterium Nostoc muscorum Singh, A. K.;D. Chakravarthy;T. P. K. Singh;H. N. Singh
  12. Physiol. Plant. v.101 Heavy metal-induced accumulation of free proline in metal-tolerant and a nontolerant ecotype of Silene vulgaris Schat, H.;S. S. Sharma;R. Vooijs
  13. New Phytol. v.143 Heavy metal-induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris Mehta, S. K.;J. P. Gaur
  14. J. Phycol. v.34 Role of proline accumulation in response to toxic copper in Chlorella sp. (Chlorophyceae) cells Wu, J. T.;M. T. Hsieh;L. C. Kow
  15. J. Plant Physiol. v.149 Effects of various salts and of mannitol on ion and proline accumulation in relation to osmotic adjustment in rice(Oryza sativa L.) callus cultures Lutts, S.;J. M. Kinet;J. Bouharmont
  16. Plant Physiol v.108 Overexpression of ${\Delta}^1$-pyrroline-S-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plant Kavi Kishor, P. B.;Z. Hong;G. H. Miao;C. A. Hu;D. P. S. Verma
  17. Plant Physiol. Biochem. v.31 Effect of copper on fatty acid composition and peroxidation of lipids in the roots of copper tolerant sensitive Silene cucubalus De Vos, C. H. R.;W. M. Ten Bookum;R. Vooijs;H. Schat;L. J. De Kok
  18. Phytoplankton Manual Counting slides Guillard, R. R. L.;A. Sournia(ed.)
  19. Plant and Soil v.39 Rapid determination of free proline for water-stress studies Bates, L. S.;R. P. Waldren;I. D. Teare
  20. J. Plant Physiol. v.135 Copper-induced damage to the permeability barrier in roots of Silene cucubalus De Vos, C. H. R.;H. Achat;R. Vooijs;W. H. O. Ernst
  21. J. Phycol. v.31 Enhancement of intracellular proline level in cells of Anacystis nidulans(Cyanobacteria) exposed to deleterious concentrations of copper Wu, J. T.;S. C. Chang;K. S. Chen
  22. Botanica Marina v.29 Copper accumulation in copper-tolerant and nontolerant populations of the marine fouling alga, Ectocarpus siliculosus(Dillw.), Lyngbye Hall, A.
  23. Arch. Microbiol. v.150 Algae sequester metal complexes via synthesis of phytochelatin complexes Gekeler, W.;E.Grill;E. L.Winnackear;M. H. Zenk
  24. Environ. Exp. Bot. v.22 An X-ray energy dispersive study of cellular compartmentalization of lead and zinc in Chlorella saccharophylla, Chlorophyta, Navicula incerta and Nitzschia closterium, Bacillariophyta Jensen, T. E.;J. W. Rachlin;V. Jani;B. Warkentine
  25. Phytochem. v.33 Proline accumulation in what seedlings exposed to zinc and copper Bassi, R.;S. S. Sharma
  26. J. Physiol. v.32 The importance of chemical speciation : From the bulk solution to the cell surface Twiss, M. R.
  27. Plant Physiol. Biochem. v.32 Water relations, gas exchange and amino acid content in cadmium-treated lettuce Costa, G.;J. L. Morel
  28. Phytochem. v.28 Hydroxyl radical scavenging activity of compatible solutes Smirnoff, N.;Q. J.;Cumbes
  29. Chim. Acta v.32 Plants which accumulate metals. IV. A possible copper-proline complex from the roots of Armeria meritima Farago, M. E.;W. A. Mullen
  30. J. Plant Physiol. v.148 Mechanism of oxygen activation during plant stress : Biochemical effects of air pollutants Hippeli, S.;E. F. Elstner
  31. Toxic Effects of Metals Plants and the chemical elements, biochemistry, uptake, tolerance and toxicity Vangronsveld, J.;H. Clijsters;M. E. Farato(ed.)
  32. Physiol. Plant. v.96 Oxidative damage and defence mechanisms in primary leaves of Phaseolus vulgaris as a result of root assimilation of toxic amounts of copper Weckx, J. E. J.;H. M. M. Clijsters
  33. Physiol. Plant. v.106 Copper affects the enzymes of the ascorbate-glutathione cycle and its related metabolites in roots of Phaseolus vulgaris Gupta, M.;A. Cuypers;J. Vangronsveld;H. Clijsters
  34. Environment and Plant Metabolism Proline accumulation during drought and salinity Samaras, Y.;R. A. Bressan;L. N. Csonka;M. G. Garcia-Ris;M.Paino Durzo;D.Rhodes;N. Smirnoff(ed.)
  35. J. Plant Physiol. v.140 Protection of membrane integrity in Medicago sativa L. by glycinebetaine against the effects of freezing Zhao, Y.;D. Aspinall;L. G. Paleg