The Action of ATP on Phospholipase $A_2$Activation in C6 Cells

C6세포에서 phospholipase $A_2$활성에 대한 ATP의 작용

  • 심상수 (중앙대학교 약학대학 병태생리학교실) ;
  • 김명준 (가톨릭대학교 의과대학 생리학교실) ;
  • 윤신희 (가톨릭대학교 의과대학 생리학교실) ;
  • 김창종 (중앙대학교 약학대학 병태생리학교실) ;
  • 조양혁 (가톨릭대학교 의과대학 생리학교실)
  • Published : 2001.08.01

Abstract

To investigate action of ATP on ischemia-induced brain injury, we measured phospholipase $A_2$activity and nitric oxide (NO) production in C6 cells. ATP alone did not have any influence on phospholipase $A_2$activity but increased NO production. Glutamate (1 mM) significantly increased phospholipase $A_2$activity whereas did not increased NO production. ATP significantly inhibited phospholipase $A_2$activation induced by 0.1 $\mu$M A23187, 1 mM glutamate and 1 mM $H_2O$$_2$, but did not inhibited 1 $\mu$M PMA-induced phospholipase $A_2$activation. From the above results, it is suggested that the action of ATP in C6 cells has dual actions, such as the inhibition of agonist-induced phospholipase $A_2$activation and the increase of NO production.

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