Enhancement of Xylitol Production Yield by Xylitol Dehydrogenase Defective Mutant of Pichia stipitis

Pichia stipitis의 Xylitol Dehydrogenase Defective Mutant에 의한 Xylitol 생산 수율 향상

  • Kim, Min-Soo (Department of Molecular Science and Technology, Division of Chemical Engineering and Biotechnology, College of Engineering, Ajou University) ;
  • Kim, Chul (Department of Molecular Science and Technology, Division of Chemical Engineering and Biotechnology, College of Engineering, Ajou University) ;
  • Seo, Jin-Ho (Department of Food Science and Technology, Seoul National University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Division of Chemical Engineering and Biotechnology, College of Engineering, Ajou University)
  • 김민수 (아주대학교 화학생물공학부 및 분자과학기술학과) ;
  • 김철 (아주대학교 화학생물공학부 및 분자과학기술학과) ;
  • 서진호 (서울대학교 식품공학과) ;
  • 유연우 (아주대학교 화학생물공학부 및 분자과학기술학과)
  • Published : 2001.06.01

Abstract

In order to produce xylitol with high yield, experiments were carried out to develope xylitol dehydrogenase(XDH) defective mutant from P. stipitis and to investigate the xylitol fermentation characteristics of mutant strain. After treatment of P. stipitis with EMS, mutant PXM-4 was selected based on te XDH activity and xylitol production capability. Among the tested cosubstrates, galactose was selected as an adequate cosub-strate on xylitol production of mutant PXM-4. But with the increase in the concentration of galactose in the medium, xylitol production was decreased because the transport of xylose into cell was inhibited by galactose. The optimal concentration of galactose for the production of xylitol using 20 g/ι xylose was 20 g/ι Under this condition, maximum concentration of xylitol and yield were 14.4 g/ι and 97%, respectively. In order to prevent the inhibitory effect of xylose transport by galactose, galactose was fed with low concentration and the concentration of xylitol produced was increased up to 25 g/ι.

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