Production of Portopectinase from Bacillus subtilis EK11

Bacillus subtilis EK11로부터 Protopectinase 생산

  • 문철환 (경남대학교 자연과학대학 생명과학부) ;
  • 최종수 (경남대학교 자연과학대학 생명과학부) ;
  • 이승철 (경남대학교 자연과학대학 생명과학부) ;
  • 황용일 (경남대학교 자연과학대학 생명과학부)
  • Published : 2001.09.01


In plant tissues intercellular cementing portion called as middle lamella consists of high proportion of protopectin that is water insoluble form of pectin on their backbone Protopectinase (PPase) a heterogeneous group of enzymes that hydrolyze or dissolve the insoluble protopectin in plant tissues by restricted depolymerization liberates water solu- ble pectin with the resultant separation of plant tissues that have been protected against environmental shock by rigid cel wall . Bacillus subtilis EKll was most effective for PPase Production For increasing of PPase productivity effects of glucose concentrations, pHs and aeration rates were studied in batch culture The most proper concentra- tion of glucose pH and air condition for PPase Production were 1% 8.0 and lvvm respectively In these condi- tion PPase productivity was $84,364 UL^{-1}$ $h^{-1}$ and increased about 15.6 times than flask fermentation.


Bacillus subtilis EK11;portopectinase;fed-batch culture


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