- Volume 29 Issue 3
Effects of Bifidobacteria on the Growth and Caco-2 Cell Adherence of E. coli O157:H7
Bifidobacteria 가 E. cold O157:H7의 생육 및 Caco-2 세포 정착에 미치는 영향
- 김응률 (매일유업(주) 중앙연구소) ;
- 정후일 (매일유업(주) 중앙연구소) ;
- 전석락 (매일유업(주) 중앙연구소) ;
- 유제현 (건국대학교 낙농학과)
- Published : 2001.09.01
This study was conducted to investigate the effects of bifidobacteria on the growth and Caco-2 cell-adherence of Escherichia coli O157:H7 .Dur-ing momo-culture of E. coli O157:H7 and mixed culture with Bifidobacterium infantis K9, pH viable cell count, and ammonia concentration were measured Co-cultivation of E. coli O157:H7 with bifidobacteria. producing acidic metabolites rapidly decreased the viable cell count of E. coli O157:H7 In addition rapid decrease of ammo- nia concentration was observed during mixed culture after 8 hrs incubation compared to single culture of E. coli O157:H7 Therefore it is likely that bifidobacteria assimilate ammonia produced by E. coli O157:H7 P4 B, infantis K9 showed quite similar adherence on the Caco-2 cells in either case. On the other hand adherence of E. coli O157:H7 decreased from 2.6% to 1.86% when B infantis K9 was adhered to Caco-2 cell 2 hrs prior to the application of E. coli O157:H7 In conclusion in adherence of E coli O157:H7 to Caco -2 cell was inhibited by competition of its binding to the adherence site with bifidobacteria. In addition inhibitory effects of bifidobacteria on E coli O157:H7 appeared to be much higher with increae of the number of bifidobacteria and its ability of adherence to Caco-2 cells.
Bifidobacteria;E. coli O157:H7;growth inhibition;Caco-2 cell adherence
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