Effects of Bifidobacteria on the Growth and Caco-2 Cell Adherence of E. coli O157:H7

Bifidobacteria 가 E. cold O157:H7의 생육 및 Caco-2 세포 정착에 미치는 영향

  • 김응률 (매일유업(주) 중앙연구소) ;
  • 정후일 (매일유업(주) 중앙연구소) ;
  • 전석락 (매일유업(주) 중앙연구소) ;
  • 유제현 (건국대학교 낙농학과)
  • Published : 2001.09.01


This study was conducted to investigate the effects of bifidobacteria on the growth and Caco-2 cell-adherence of Escherichia coli O157:H7 .Dur-ing momo-culture of E. coli O157:H7 and mixed culture with Bifidobacterium infantis K9, pH viable cell count, and ammonia concentration were measured Co-cultivation of E. coli O157:H7 with bifidobacteria. producing acidic metabolites rapidly decreased the viable cell count of E. coli O157:H7 In addition rapid decrease of ammo- nia concentration was observed during mixed culture after 8 hrs incubation compared to single culture of E. coli O157:H7 Therefore it is likely that bifidobacteria assimilate ammonia produced by E. coli O157:H7 P4 B, infantis K9 showed quite similar adherence on the Caco-2 cells in either case. On the other hand adherence of E. coli O157:H7 decreased from 2.6% to 1.86% when B infantis K9 was adhered to Caco-2 cell 2 hrs prior to the application of E. coli O157:H7 In conclusion in adherence of E coli O157:H7 to Caco -2 cell was inhibited by competition of its binding to the adherence site with bifidobacteria. In addition inhibitory effects of bifidobacteria on E coli O157:H7 appeared to be much higher with increae of the number of bifidobacteria and its ability of adherence to Caco-2 cells.


Bifidobacteria;E. coli O157:H7;growth inhibition;Caco-2 cell adherence


  1. Antonie van Leeuwenhoek v.50 The function of Lactobacillus as dietary adjuncts Bol, J.
  2. Bifido. Microflora. v.1 Recent trends in research on intestinal flora Mitsuoka, T.
  3. Gut v.35 Lactobacillus acidophilus LA1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterivirulent bacteria Bernet, M. F.;D. Brassart;J. R. Nesser;A. L. Servin
  4. Biosci. Biotech. Biochem. v.60 Immune functions of immunoglobulin Y isolated from egg yolk of hens immunized with various infectious bacteria Konishi, Y. S.;K. Shibata;S. S. Yun;Y. H. Kudo;K. Yamaguchi;S. Kumagai
  5. Lett. Appl. Microbiol. v.21 Adhesion of different bifidobacteria strains to human entero-cyte-like Caco-2 cells and comparison with in vivo strdy Crociani, J.;J. P. Grill;M. Huppert;J. Ballongue
  6. Bifido. Microflora. v.14 Inhibitory effects of Bifidobacterium longum BB536 on harmful intestinal bacteria Kojima, T. A.;T. Yaeshima;N. Ishibashi;S. Shimamura;H. Hayasawa
  7. Appl. Environ. Microbiol. v.59 Adhesion of human bifidobacterial strains to culturek human intestinal epothelial cells amd inhibition of enteropathogen-cell interactions Bernet, M. F.;D. Brassart;J. R. Neeser;A. L. Servin
  8. Dairy Food Environ. Sanit. v.12 HACCP-An industry food safety self-control program,part Ⅲ Snyder, O. P.
  9. Agr. Biol. Chem. v.50 Studies on growth promoting substances for L. bifidus found in cultured medium of E.coli. Yamashita, M.;M. Sasaki;K. Hamada;H. Ueno
  10. Agr. Biol. Chem. v.36 Adhesion of lactic acid bacteria to cells mediated by sugar chains Yamamoto, K.
  11. Bifido. Microflora. v.10 Current status and future trends of bifidobacteria related research and products in the USA Hoover, D. G.;D. B. Hughes
  12. Biosci. Microflora. v.16 Adhesion of two Lactobacillus strains, one Lactococcus, and one Propionibacterium strain to cultured human intestinal Caco-2 cell line Lehto, E. M.;S. Salminen
  13. FEMS Microbiol. Lett. v.91 Competitive exclusion of diarrheagenic Escherichia coli(ETEC) from enterocyte-like Caco-2 cells by heat-killde Lactobacillus Chauviere, G.;M. H. Coconnier;S. Kerneis;A. Darfeuille-Michaud;B. Joly;A. L. Servin
  14. Cult. Dairy. Prod. J. v.23 Characterization of lactobacilli and bifidobacteria as applied to dietary adjuncts Kim, H. S.
  15. J. Food Sci. v.43 Growth of Bifidobacterium var.pennsylvanicus in laboratory media supplemented with amino sugars and spent broth from Escherichia coli Jao, Y. C.;E. M. Mikolajcik;P. M. T. Hansen
  16. Kor. J. Appl. Microbiol. Biotechnol. v.27 Effects of Bifidobacterium longum HY8001 against Escherichia coli O157:H7 and Salmonella typhimurium DT104 enteric infection and evaluation of vero cytotoxin neutralizing effects Yang, S. J.;J. W. Yoon;K. S. Seo;H. C. Koo;S. H. Kim;H. S. Bae;Y. J. Baek;Y. H. Park
  17. Appl. Environ. Microbiol. v.61 Genes involved in immunity to the lantibiotic nisin produced by Lactococcus lactis 6F3 Siegers, K.;K. D. Entian.
  18. Cult. Dairy. Prod. J. v.25 Bifidobacteria as possible dietary adjuncts in cultured dariy products - A review Laroia, S.;J. H. Martin
  19. Biosci. Microflora. v.15 Inhibitory effects of human-derived Bifidobacterium on pathogenic Escherichia coli serotype O-111 Kojima, T. A.;T. Yaeshima;N. Ishibashi;S. Shimamura;H. Hayasawa
  20. Bifido. Microflora. v.8 Stress and intestinal flora Suzuki, V.;Y. Kodama;T. Mitsuoka
  21. Neth. Milk Dairy. J. v.43 Fermented milks with selected intestinal bacteria: A healthy trend in new products Driessen, F. M.;R. De Boer