Isolation of Keratinolytic Protease Producing Microorganism and Its Cultivation Condition

Keratinolytic protease 생산균, Pseudomonas sp. KP-364의 분리 및 배양

  • 전동호 (건국대학교 공과대학 미생물공학과) ;
  • 권태종 (건국대학교 공과대학 미생물공학과)
  • Published : 2001.09.01


A bacterial strain KP-364 producing extracellular keratinolytic protease was isolated from the soil of the poultry fac-tory. It was identified as Pseudomonas sp. based on its morphological and physiological characteristics, The optimal culture conditions for the production of keratinolytic protease by Pseudomonas sp. KP-364 were investigated. The composition of optimal medium for the keratinolytic protease was 2.0% glucose, 0.5% soybean meal. 0.5% $NaNO_3$ and 0.2% KCI Optimal initial pH for production of Keratinolytic protease production were 6.5 and $37^{\circ}C$ respec- tively. The keratinolytic protease production reached a maximum of 1,270 U/ml/hr after 48 hours cultivation under the optimal culture conditions.


Keratinolytic protease;isolation;keratin


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