Isolation of Keratinolytic Protease Producing Microorganism and Its Cultivation Condition

Keratinolytic protease 생산균, Pseudomonas sp. KP-364의 분리 및 배양

  • 전동호 (건국대학교 공과대학 미생물공학과) ;
  • 권태종 (건국대학교 공과대학 미생물공학과)
  • Published : 2001.09.01

Abstract

A bacterial strain KP-364 producing extracellular keratinolytic protease was isolated from the soil of the poultry fac-tory. It was identified as Pseudomonas sp. based on its morphological and physiological characteristics, The optimal culture conditions for the production of keratinolytic protease by Pseudomonas sp. KP-364 were investigated. The composition of optimal medium for the keratinolytic protease was 2.0% glucose, 0.5% soybean meal. 0.5% $NaNO_3$ and 0.2% KCI Optimal initial pH for production of Keratinolytic protease production were 6.5 and $37^{\circ}C$ respec- tively. The keratinolytic protease production reached a maximum of 1,270 U/ml/hr after 48 hours cultivation under the optimal culture conditions.

Keywords

Keratinolytic protease;isolation;keratin

References

  1. Biochem. J. v.232 Purification and characterization of major extracellular proteinases from Trichophyton rubrum Masakazu, A.;R. Lindquist;K. Fukuyama;F. Apodaca;W. L. Epstein;J. H. McKerrow
  2. Dermatol. v.196 The in vitro and in vivo production of a 31.5-kd keratiolytic subtilase from Microsporum canis and the clinical status in naturally infected cats Mignon, B. R.;A. F. Nikkels;G. E. Pierard;B. J. Losson
  3. Appl. Environ. Microbiol. v.58 Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain Xiang, L.;C. G. Lee;E. S. Casale;J. C. H. Shih
  4. Med. Microbiol. Immunol. v.180 Quantification of keratinolytic activity from Dermatophilus congolensis Heinz, H.;J. Kalisch;M. Keil;W. C. Marsch;M. Buslau
  5. Br. J. Dermatol. v.134 The role of two endogenous proteases of the stratum corneum in degradation of desmoglein-1 and their reduced activity in the skin of ichthyotic patients Suzuki, Y.;J. Koyama;O. Moro;I. Horii;K. Kikuchi;M. Tanida;H. Tanida;H. Tagami
  6. Arch. Biochem. Biophysics. v.135 Amino acid composition and specificity of a keratinase of Trichophyton mentagrophytes Ruey, J. Y.;S. R. Harmon;P. E. Wachter;F. Blank
  7. J. Bacteriol. v.96 Isolation amd Purification of an extracellular keratinase of Trichophyton muntagrophytes Yu, R. J.;S. R. Harmon;F. Blank
  8. Williams and Wilkins Bergey's Manual of Determinative Bacteriology, 9th ed. John, G. H.;N. R. Krieg;P. H. A. Sneath
  9. Ann. Rev. Cell Develop. Biol v.11 Keratins and the skin Elaine, F.
  10. Keratinolysis by poultry farm soil fungi. Mycopathol v.139 Kaul, I.;G. Sumbali
  11. J. Invest. Dermatol. v.53 Hair digestion by a keratinase of Trichophyton mentagrophytes Ruey, J. Y.;S. R. Harmon;F. Blank;S. Nat;S. Techn
  12. Med. Microbiol. Immunol v.180 Quantification of keratinolytic activity from Dermatophilus congolensis Hanel, H.;J. Kalisch;M. Keil;W. C. Marsch;M. Buslau
  13. Arch. Dermatol. Res. v.285 Detection and characterization of endogenous protease associated with desquamation of stratum comerum Suzuki, Y.;J. Nomura;J. Hori;J. Koyama;M. Takahashi;I. Horri
  14. Biosci. Biotech. Biochem v.56 Degration of Human Hair by a thermostable alkaline protease form alkaliphilic Bacillus sp. No. AH-101 Tideto, T.;S. Nakamura;R. Aono;K. Horikoshi
  15. Biodegr. v.37 digestion of keratin by Pseudomonas sp.Inter.Biodeter Rosa, S. P.;F. Bicca;P. C. Santos;S. K. Ferrao;H. Dewes;C. C. Gpylarde;C. Termignoni;R. W. S. P. Thomas
  16. J. Med. Veter. Mycol. v.25 Intracellular keratinase of Trichophyton gallinae Krystyna. W.;J. Lobarzewski;T. Wolski
  17. Yonsei Med. J. v.28 Isolation, purification and characterization of keratinolytic proteinase from Microsporum canis Lee, K. H.;K. K. Park;S. H. Park;J. B. Lee
  18. Appl. Environ. Microbiol v.61 Characterizaion of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530 Brigitte, B.;B. Galunsky;R. Muller
  19. Comp. Biochem. Physiol v.77 Puripication and some properties of an acidic protease from epimastigtes of Trupanosoma cruzi Esteban, B.;M. Berta
  20. Appl. Environ. Microbiol. v.56 Isolation, identification, and characterization of a feather-degrading bacterium Williams, C. M.;C. S. Richter;J. M. Mackenzie;J. C. H. Shih
  21. Prog. Histochem. Cytochem. v.25 Cohesion amd desquamation of epidermal stratum corneum Brysk, M. M.;S. Rajaraman
  22. Microbial ecology. The prokaryotes A: handbook on habitats, isolation, and identification of bacteria Mortimer P. S.
  23. Appl. Environ. Microbiol. v.61 Nucleotide sequence and expressing of KerA,the gene encoding a keratinolytin protease of Bacillus Iicheniformis PWD-1 Xing, L.;D. W. Kelemen;E. S.miller;J. C. H.Shih
  24. Mycoses. v.38 Comparative studies on keratinase production of Trichophyton mentagrophytes strains of animal origin Ute, S.;K. H. Bohm
  25. Acta. Derm. Venereol. v.71 Stratum corneum chymotryptic enzyme: a proteinase which may be generally present in the stratum corneum and with a possible involvement in desquamation Lundstrom, A;T. Egelrud
  26. J. Dermatol. v.14 a New keratinolytic proteinase from clinical isolates of Trichophyton mentagrophytes Ryoji, T.;I. J. Ko;K. Matsuda;H. Ogawa
  27. Mycoses. v.40 Extracellular enzyme activities of dermatophytes and yeast isolates on solid media Muhsin, T. M.;A. H. Aubaid;A. H. Al-Duboon
  28. Can. J. Microbiol. v.21 Degradation of feather keratin by culture filtrates of Streptomyces fradiae Young, R. A.;R. E. Smith
  29. J. Bacteriol v.117 Phosphate-mediated alteration of the Microsporum gypseum germination protease specificity for substrate: Enhanced keratinase activity Page, W. J.;J. J. Stock