Antimicrobial Activity of Culture Filtrates from Pseudomonas aeruginosa KLP-2 Isolated from Cooling Tower-Water against Legionella pneumophila

냉각탑수에서 분리한 Pseudomonas aeruginosa KLP-2 배양여액의 Legionella Pneumophila에 대한 항균활성

  • 박은희 (부산광역시 보건환경연구원 미생물과) ;
  • 차인호 (부산광역시 보건환경연구원 미생물과) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 2001.09.01

Abstract

Pseudomonas aeruginosa KLP-2 possessing antimicro- bial activity against Legionella pneumophila was isolated from cooling tower-waters. The culture filtrates of P. aeruginosa KLP-2 showed antimicrobial activity against L. pneumophila Vibro cholerae non-Ol. Bacillus cereus, Bacillus subtilis and staphylococcus aureus. The culture filtrates of P. aeruginosa KLP-2 showed the highest anti-microbial activity against. L pneumophila among micoorganisms tested in this study. The optimal conditions of temperature, pH carbon source and nitogen source to obtain maximal antimicrobial activity from culture filtrates of P. aeruginosa KLP-2 were determined to be 35$^{\circ}C$ pH 7.0 % of glycerol and 0.6% of proteose peptone respec- tively. The antimicrobial activity of culture filtrates from P. seruginosa KLP-2 was highest against L. pneumophila when cultivated with shaking for 24 h and without shaking for 4 day at 35$^{\circ}C$.

Keywords

Legionella pneumophila;Pseudomonas aeruginosa;antimicrobial acitivity

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