High-level Production of Recombinant Human IFN-$\alpha2a$ with Co-expression of $tRNA^{Arg(AFF/AGA)}$ in High-cell-density Cultures of Escherichia coli

  • Shin, Chul-Soo (Bioprocess Engineering Laboratory, Hanhyo Institutes of Technology, Yusong, Taejon 305-390) ;
  • Hong, Min-Seon (Bioprocess Engineering Laboratory, Hanhyo Institutes of Technology, Yusong, Taejon 305-390) ;
  • Shin, Hang-Chel (Department of Life Science and Bioinformatics, Soongsil University, Seoul 156-743) ;
  • Lee, Jeewon (Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB). P. O. Box 115, Yusong, Taejon 305)
  • Published : 2001.07.01

Abstract

The co-expression of the arg U gene in a double-vector expression system of recombi-nant Escherichia coli BL22(DE3)[pET-IEN2a+pAC-argU] significantly enhanced the production level of reconminant human interferon -$\alpha$2a(rhIFN-$\alpha$2a) in high cell density cultures, compared to a recombinant E. coli culture containing only the single expression vector, pET-IEN2a. The dry cell mass concentration increased to almost 100 g/L, and more than 4 g/L of rhIFN-$\alpha$2a was accumu-lated in the culture broth. Evidently, the synthesis of rhIFN-$\alpha$2a was strongly dependent on the pre-induction growtih rate and more efficient at a higher specific growth rate. The additional sup-ply of tRN $A^{Arg(AGG/AGA)}$ enhanced the expression level of the rhIFN-$\alpha$2a gene in the early stage of the post-induction phase, yet thereafter the specific production rate of rhIFN-$\alpha$2a rapidly de-creased due to severe segregational instability of plasmid vector pET-IEN2a. It would appear that the plasmid instability with only occurred to pET-IEN2a in the double vector system, was re-lated to the effect of translational stress due to the over expression of rhIFN-$\alpha$2a.

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