Toxicological Analysis of the Entomopathogenic Nematode, Steinernema carpocapsae, and the Symbiotic Bacteria, Xenorhabdus nematophilus on Beneficial Insects and Mammals

유용곤충과 포유류에 대한 곤충병원선충(Steinernema carpocapsae)과 공생세균(Xenorhabdus nematophilus)의 독성

  • Park, Young-Jin (School of Bioresource Sciences, College of Natural Sciences, Andong National University) ;
  • Kim, Mi-Kyung (Institute of Korea Biotech) ;
  • Kim, Jin (Central Research Institute, Kyung-Nong Corporation) ;
  • Yang, Kyung-Hyung (Central Research Institute, Kyung-Nong Corporation) ;
  • Kim, Yong-Gyun (School of Bioresource Sciences, College of Natural Sciences, Andong National University)
  • 박영진 (안동대학교 자연과학대학 생명자원과학부) ;
  • 김미경 (고려바이오 연구소) ;
  • 김진 ((주)경농 중앙연구소 독성연구실) ;
  • 양경형 ((주)경농 중앙연구소 독성연구실) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학부)
  • Published : 2001.09.01

Abstract

Toxicological studies of two potential biological control agents, the entomopathogenic nematode (Steinernema carpocapsae) and the symbiotic bacteria (Xenorhabdus nematophilus) were conducted against two beneficial insects and one mammal species. Two microbial agents varied in their toxicities between two insect species: an ant, Pristomyrmex pungens, and silkworm, Bombyx mori. In oral toxicity test, the symbiotic bacteria resulted in significant lethal [half lethal concentration of $1.4$\times$10^3$colony-forming units (cfu)/ml] on the ants, while they gave little lethal effect (half lethal concentration of more than $10^{8}$ cfu/ml) on the silkworms. The nematodes, however, gave significant lethal effect [half lethal concentration of 4 infected juveniles (IJs)/ml] on the silkworms, while they did little lethal effect (half lethal concentration of 150,000 IJs/ml) on the ants in topical assays. Both the nematodes and the bacteria did not give lethal effect to the albino rats, Rattus norvegicus, when they were fed orally into the rats. Also, any of these microbial agents were not detected in the internal organs of the treated rats.

Keywords

Biological control;Entomopathogenic nematode;Entomopathogenic bacteria;Toxicity;Risk assesment