Comparison of Jujube Extract with Tangerine Peel Extract in Lowering Plasma Lipids and Activities of Cholesterol Regulating Enzymes in Cholesterol-Fed Rats

  • Park, Myung-Sook (Department of Nutrition and Food Science, Kyungpook National University) ;
  • Bok, Song-Hae (Korea Research Institute of Bioscience & Biotechnology)
  • Published : 2001.06.01

Abstract

A potential mechanism through which the jujube extract might produce a cholesterol-lowering effect was compared with that of tangerine peel extract in vivo. Two extracts were prepared using ethanol. Male rats were fed a high cholesterol (1%, w/w) lab chow with jujube extract (1.2%) or tangerine peel extract (6.3%, w/w) for 3 weeks. Activities of hepatic HMG-CoA reductase (289.6$\pm$12.9 and 296.7$\pm$11.6 nmole/min/mg vs. 347.9$\pm$17.5 nmole/min/mg) and ACAT (554.8$\pm$18.2 and 451.7$\pm$19.4 nmole/min/mg vs. 602.6$\pm$21.4 nmole/min/mg) were significantly lowered by both supplements compared to the control group. These two supplements also substantially reduced the concentrations of plasma cholesterol (103.3$\pm$15.9 and 101.6$\pm$19.4 mg/dL vs. 141.6$\pm$18.1 mg/dL) and triglyceride (61.3$\pm$5.5 and 55.5$\pm$3.9 mg/dL vs. 96.0$\pm$4.2 mg/dL). The inhibition of HMG-CoA reductase resulting from the supplementation of jujube or tangerine extracts could count for the reduction in plasma cholesterol. Accordingly, lipid-lowering action of both supplements appears to be similar in high-cholesterol fed rats.

References

  1. Atherosclerosis v.118 no.Suppl Serum cholesterol and ischaemic heart disease Wald, N.J.;Law, M.R.
  2. Pharmacology v.46 MG 12561 (Dalvastatin) : A novel synthetic inhibitor of HMG-CoA reductase and cholesterol-lowering agent Amin, D.;Gustafson, S.K.;Weinacht, J.M.;Cornell, S.A.;Neuenschwander, K.;Scotese, A.C.;Regan, J.R.;Perrone, M.H.
  3. Am. J. Cario. v.2 Discovery, biochemistry and biology of lovastatin Alberts, A.W.
  4. Biochim. Biophy. Acta. v.1254 The mechanism of lack of hypocholesterolemic effects of pravastatin sodium, a 3-hydroxy-3-methylglutaryl cocnzyme A reductase inhibitor, in rats Fujioka, T.;Nara, F.;Tsujita, Y.;Fukushige, J.;Fukami, M.;Kuroda, M.
  5. Arch. Intern. Med. v.153 I through Ⅳ, Lovastatin 5-year safety and efficacy study Lovastatin study groups
  6. Biochim. Biophy. Acta. v.1167 Effect of fluvastatin sodium (XU62-320), a new inhibitor of 3 hydroxy-3-methyl glutaryl coenzyme A reductase, on the induction of low-density lipoprotein receptor in HepG2 cells Hayashi, K.;Kurokawa, J.;Nomura, S.;Kuga, Y.;Ohkura, Y.;Kajiyama, G.
  7. J. Lipid Res. v.26 Role of acyl-CoA : cholesterol acyltransferase in cellular cholesterol metabolism Suckling, K.E.;Stange, E.F.
  8. J. Lipid Res. v.245 Role of acyl CoA: cholesterol acyltransferase in cholesterol absorption and its inhibition by 57-118 in the rabbit Heidner, J.G.;Pickens, C.E.;Kelly, L.A.
  9. Comp. Biochem. Physiol. A v.99 Effects of acyl-CoA:cholesterol O-acyltransferase inhibition of cholesterol absorption and plasma lipoprotein composition in hamsters Schnitzer-Polokoff, R.;Compton, D.;Boykow, G.;Davis, H.;Buttier, R.
  10. Atherosclerosis v.113 TMP-153, a novel ACAT inhibitor, inhibits cholesterol in rats and hamsters Sugiyama, Y.;Ishikawa, E.;Odaka, H.;Miki, N.;Tawada, H.;Ikeda, H.
  11. Cancer Res. v.41 Activation and inhibition of benzo (a)pyrenc and aflatoxin B1 metabolism in human liver microsomes by naturally occurring flavonoids Buening, M.K.;Chang, R.L.;Huang, M.T.;Forther, J.G.;Wood, A.W.;Conney, A.H.
  12. J. Pharm. Pharmacol. v.46 Pharmacological evaluation of th anti-inflammatory activity of a citrus bioflavonoid, hesperidin, and the isoflavonoids, duartin and claussequinone in rats and mice Emim, J.A.;Oliveira, A.B.;Lapa, A.J.
  13. J. Nutr. v.129 Plasma and hepatic choleserol and hepatic activities of 3-hydroxy-3-methylglutaryl-CoA reductase and acyl CoA:cholesterol acltransferase are lower in rats fed citrus peel extract or a mixture of citrus bioflavonoids Bok, S.H.;Lee, S.H.;Park, Y.B.;Bae, S.H.;Son, K.H.;Jeong, T.S.;Choi, M.S.
  14. Nutr. Res. v.19 Hypocholesterolemic effect of hesperetin mediated by inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase and acyl coenzyme A : cholesterol acyltransferase in rats fed high-cholesterol diet Lee, S.H.;Jeong, T.S.;Park, Y.B.;Kwon, Y.K.;Choi, M.S.;Bok, S.H.
  15. Clin. Chem. v.20 Enzymatic determination of total serum cholesterol Allain, C.C.;Poon. L.S.;Chan, C.S.G.;Richman, W.;Fu, P.C.
  16. J. Lipid Res. v.19 A comprehensive evaluation of the heparin-manganease precipitation procedure for estimating high density lipoprotein cholesterol Warnick, R.G.;Albers, J.J.
  17. Clin. Chem. v.29 A peroxidase-coupled method for the colorimetric determination of serum triglycerides McGowan, M.W.;Artiss, J.D.;Strandbergh, D.R.;Zak, B.
  18. J. Lipid Res. v.14 Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A Hulcher, F.H.;Oleson, W.H.
  19. Anal. Biochem. v.72 A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.M.
  20. Biochim. Biophy. Acta. v.370 Micro assay for 3-hydroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts Shapiro, D.J.;Nordstron, J.L.;Mitschelen, J.J.;Rodwell, V.W.;Schimke, R.T.
  21. J. Lipid Res. v.21 Rat liver acyl-coenzyme A: choleserol acyltransferase: its regulation in vivo and some of properties in vitro Erickson, S.K.;Schrewsbery, M.A.;Brooks, C.;Meyer, D.J.
  22. Exp. Mole. Pathol. v.44 Regulation of acyl-CoA : Cholesterol acyltransferase activity in normal and atherosclerotic rabbit aortas: Role of a cholesterol substrate pool Gillies, P.J.;Rathgeb, K.A.;Robinson, C.S.
  23. Bio. Biotech. Biochem. v.61 Hesperidin as an inhibitor of lipase from porcine pancreas and Pseudomonas Kawaguchi, K.;Mizuno, T.;Aida, K.;Uchino, K.
  24. Farmaco. v.50 Biological effects of hesperidin, a citrus flavonoid (note Ⅱ): hypolipidemic activity on experimental hypercholesterolemia in rat Monforte, M.T.;Trovato, A.;Kirjavainen, S.;Forestieri, A.M.;Galati, E.M.;Lo Curto, R.B.
  25. Archiv. Biochem. Biophy. v.325 Inhibitors of cholesterol biosynthesis increase hepatic low-density lipoprotein receptor protein degradation Ness, G.C.;Zhao, Z.;Lopez, D.
  26. Proc. Natl. Acad Sci. USA v.83 Mevinolin, and inhibitor of cholesterol synthesis, induces mRNA for low density lipoprotein receptor in livers of hamsters and rabbits Ma, P.T.S.;Gil, G.;Sudhog, T.C.;Bilheimer, D.W.;Goldstein, J.L.;Brown, M.S.
  27. Biochim. Biophy. Acta. v.575 Effects of ML-236B on cholesterol metabolism in mice and rats : lack of hypocholesterolemic activity in normal animals Endo, A.;Tsujita, Y.;Kuroda, M.;Tanzawa, K.
  28. J. Lipid Res. v.34 Plasma cholesteryl ester transfer protein Tall, A.R.
  29. Lipids v.30 The effects of simvastatin and cholestyramine, alone and in combination, on hepatic cholesterol metabolism in the male rat Shand, J.H.;West, D.W.
  30. vivo v.8 In vivo effect of dietary factors on the molecular action of aflatoxin B: role of non-nutrient phenolic compounds on the catalytic activity of liver fraction Aboobaker, V.S.;Balgi, A.D.;Bhattacharya, R.K.