Effects of Omahwan(OMH) on CD4+, CD8+ T cell and Immune Organ Index in Rat

오마환(烏麻丸)이 백서(白鼠)의 혈액내(血液內) CD4+, CD8+ T cell 및 면역기관(免疫器官) 장기지수(臟器指數)에 미치는 영향(影響)

  • Lee Song-Shil (Dept. of Preventive Medicine, College of Oriental Medicine, Kyung-Hee University,Institute of Oriental Medicine, Kyung-Hee University) ;
  • Lee Sang-Jae (Dept. of Preventive Medicine, College of Oriental Medicine, Kyung-Hee University,Institute of Oriental Medicine, Kyung-Hee University) ;
  • Kim Kwang-Ho (Dept. of Preventive Medicine, College of Oriental Medicine, Kyung-Hee University,Institute of Oriental Medicine, Kyung-Hee University)
  • 이송실 (경희대학교 한의과대학 예방의학교실,경희대학교 한의학연구소) ;
  • 이상재 (경희대학교 한의과대학 예방의학교실,경희대학교 한의학연구소) ;
  • 김광호 (경희대학교 한의과대학 예방의학교실,경희대학교 한의학연구소)
  • Published : 2001.06.29

Abstract

OMH which is known for its properties of recruiting vitality, is composed of Polygonum multiflorum Thunb. and Sesamum indicum DC.. This formula is known to possess the properties of recruiting vitality, blackening white hair and expanding life span. 16-week-old SD-Rats were treated with OMH for 16 days. After 24 hours, the rats were treated with MTX(Mthotrexate is oral administrated for 4 days(1mg/kg/day), in order to lower immunity. Then, These rats were classified in to groups, the N-16 group(not specially tested), the Control group(MTX), the OMH-L group(2.5% OMH+MTX)&the OMH-H group(10% OMH+MTX) and 6 rats were assigned to each group. After 18 hours from MTX treatment the organ index of the rats from each group Thymus and Spleen were measured. The percentage of CD+4, CD8+ T cell were measured and compared by flow cytometer. 1) Rats from the OHM-L&OMH-H group showed higher organ indexes of the Thymus and Spleen compared to the rats from the Control Group. This proves that OMH possesses the properties to mitigate degenerations of immunity($F_{thymus}=20.162,\;F_{spleen}=5.882$, ANDVA, p<0.05). 2) The rats from the two OMH groups showed higher rates of CD4+ T cell counts compared to the control group(F=26.906, ANOVA, p<0.05). CD8+ T cell showed lower rates compared to the Control group, but showed no differences within the two OMH groups(F=1.254, ANOYA, p>0.05). CD4+/CD8+ showed higher rates in the two OMH groups compared to the control group which can be thought as a proof that OMH prevents depression of immune response(F=10.554, ANOVA, p<0.05). In this test 16 week-old rats were used, which can be considered as the middle and prime age of the human being. These rats were treated with OMH which ended up showing properties of mitigating degeneration of immune responses and maintaining T-cell rates within the blood. It was possible to study that OMH possesses the properties to increase immune responses.

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